'The 24-kDa form of basic fibroblast growth factor inhibits the migration of endothelial cells and mammary carcinoma cells while continuing to promote cell proliferation. This molecule consists of the 18-kDa fibroblast growth factor sequence plus an additional 55 amino acids at the amino-terminal end. Antibody neutralization studies suggested that the ... More
Yeast-secreted bovine herpesvirus type 1 glycoprotein D has authentic conformational structure and immunogenicity.
AuthorsZhu X, Wu S, Letchworth GJ 3rd
JournalVaccine
PubMed ID9178470
'Bovine herpesvirus-1 (BHV-1) glycoprotein D (gD), an envelope glycoprotein, engenders mucosal and systemic immunity protecting cattle from viral infection. Production of gD with authentic immunogenicity is required for a subunit vaccine. We placed the truncated BHV-1 gD gene, lacking its putative transmembrane and cytoplasmic domains, under the control of the ... More
Expression of functional mouse 5-HT5A serotonin receptor in the methylotrophic yeast Pichia pastoris: pharmacological characterization and localization.
AuthorsWeiss HM, Haase W, Michel H, Reilander H
JournalFEBS Lett
PubMed ID8549774
'The methylotrophic yeast Pichia pastoris was tested for heterologous expression of the mouse 5-HT5A receptor. Three different expression plasmids were constructed where the cDNA of the receptor was cloned under the transcriptional control of the highly inducible promoter of the P. pastoris alcohol oxidase 1 (AOX1) gen. The expression plasmids ... More
Facile production of native-like kappa-bungarotoxin in yeast: an enhanced system for the production of a neuronal nicotinic acetylcholine receptor probe.
AuthorsFiordalisi JJ, James PL, Zhang Y, Grant GA
JournalToxicon
PubMed ID8711755
'Research on the mammalian central nervous system had been hindered by the limited number and meager supply of naturally occurring toxins that can be used as pharmacological reagents. The kappa-neurotoxins in particular are not found abundantly in nature and are difficult to obtain and isolate in quantities sufficient for research ... More
Isolation and expression of a cDNA clone encoding an Alternaria alternata Alt a 1 subunit.
'Alternaria alternata is recognized as an important source of fungal aeroallergens. Alt a 1, the major allergen of this mold, is a dimer of disulfide-linked subunits that migrate in SDS-PAGE under reducing conditions at apparent M(r)s of 14,500 and 16,000. IgE antibodies to this protein are present in the sera ... More
Production and characterization of recombinant active mouse gelatinase B from eukaryotic cells and in vivo effects after intravenous administration.
AuthorsMasure S, Paemen L, Van Aelst I, Fiten P, Proost P, Billiau A, Van Damme J, Opdenakker G
JournalEur J Biochem
PubMed ID9063441
'Gelatinase B is a matrix metalloproteinase involved in tissue remodelling. When mouse cells are triggered in vitro with interleukin- 1, bacterial endotoxin, virus-mimicking double-stranded RNA or cytokine inducers, they produce gelatinase B. To test the effects of gelatinase B in vivo, the enzyme was expressed in Chinese hamster ovary (CHO) ... More
Expression of biologically active beta subunit of bovine follicle- stimulating hormone in the methylotrophic yeast Pichia pastoris.
AuthorsSamaddar M, Catterall JF, Dighe RR
JournalProtein Expr Purif
PubMed ID9268682
'Follicle-stimulating hormone (FSH), a pituitary gonadotropin, is a heterodimer composed of an alpha subunit, which is common to all the glycoprotein hormones, noncovalently associated with the hormone- specific beta subunit. The objective of the present study is to develop a recombinant DNA expression system for the beta subunit of FSH ... More
Expression of highly disulfide-bonded proteins in Pichia pastoris.
AuthorsWhite CE, Kempi NM, Komives EA
JournalStructure
PubMed ID7881900
No abstract
Interaction between echovirus 7 and its receptor, decay-accelerating factor (CD55): evidence for a secondary cellular factor in A-particle formation [published erratum appears in J Virol 1998 Jan;72(1):890]
AuthorsPowell RM, Ward T, Evans DJ, Almond JW
JournalJ Virol
PubMed ID9371589
Soluble forms of decay-accelerating factor (DAF) (CD55), the receptor for echovirus 7, were synthesized in the yeast Pichia pastoris. Purified recombinant protein containing SCR domains 2, 3, and 4, but lacking the serine/threonine rich region, was shown to block infection of susceptible cells by echovirus 7. In contrast to the ... More
Riboflavin 5'-hydroxymethyl oxidation. Molecular cloning, expression, and glycoprotein nature of the 5'-aldehyde-forming enzyme from Schizophyllum commune.
AuthorsChen H, McCormick DB
JournalJ Biol Chem
PubMed ID9242680
Vitamin B2-aldehyde-forming enzyme catalyzes oxidation of the 5'- hydroxymethyl of riboflavin to the formyl group. We have purified the enzyme from the culture media of Schizophyllum commune (ATCC 38719) by modifying the procedure of Tachibana and Oka (Tachibana, S., and Oka, M. (1981) J. Biol. Chem. 256, 6682-6685) for cell-free ... More
Characterization of recombinant alpha-galactosidase for use in seroconversion from blood group B to O of human erythrocytes.
Alpha-Galactosidase (alpha-GAL) purified from green coffee bean cleaves the terminal galactose residues from the surface of group B erythrocytes, thereby converting these cells serologically to group O cells. Such enzymatically converted red cells have been transfused into group A and O recipients as part of the first phase of FDA-approved ... More
Potency and selectivity of the cathepsin L propeptide as an inhibitor of cysteine proteases.
AuthorsCarmona E, Dufour E, Plouffe C, Takebe S, Mason P, Mort JS, Menard R
JournalBiochemistry
PubMed ID8679567
The cathepsin L propeptide (phcl-2) was expressed in Saccharomyces cerevisiae using a human procathepsin L/alpha-factor fusion construct containing a stop codon at position -1 (the C-terminal amino acid of the proregion). Since the yield after purification was very low, the cathepsin L propeptide was also obtained by an alternate procedure ... More