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Invitrogen™

pRSET A, B, & C Bacterial Expression Vectors

Catalog number: V35120
Invitrogen™

pRSET A, B, & C Bacterial Expression Vectors

Catalog number: V35120
Catalog Number
V35120
also known as V351-20
Unit Size
20 µg
Price (USD)
854.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
V35120
also known as V351-20
20 µg
854.00
Your Price:
***
Product Overview
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The pRSET vector is designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. These cells also produce T7 lysozyme to reduce basal expression of target genes. The pRSET vector offers:

• Bacteriophage T7 promoter for high-level expression
• T7 gene 10 sequence to provide protein stability
• N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody
• N-terminal Xpress™ epitope for detection with the Anti-Xpress™ Antibody
• Enterokinase cleavage site for removal of fusion tag

A set of three vectors is provided (A, B, and C). Each has the N-terminal tag coding sequence in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible System
Inducible
Inducing Agent
IPTG
Promoter
T7
Product Type
Bacterial Expression Vector
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cleavage
EK (Enterokinase) Recognition Site
Protein Tag
His Tag (6x)
Cloning Method
Restriction Enzyme ⁄ MCS
Vector
pRSET

Contents & Storage

20 µg of each pRSET A, B, & C vector is provided lyophilized. TOP10F´, BL21(DE3)pLysS, and BL21 pRSET/lacZ control E. coli stabs are also included. Store E. coli stabs at 2–8°C. All components are guaranteed stable for 6 months when properly stored.

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Citations & References