pRSET A, B, & C Bacterial Expression Vectors - Citations

pRSET A, B, & C Bacterial Expression Vectors - Citations

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Abstract
Molecular cloning and functional reconstitution of a urate transporter/channel.
AuthorsLeal-Pinto E,Tao W,Rappaport J,Richardson M,Knorr BA,Abramson RG
JournalThe Journal of biological chemistry
PubMed ID8995305
The active conformation of avilamycin A is conferred by AviX12, a radical AdoMet enzyme.
AuthorsBoll R,Hofmann C,Heitmann B,Hauser G,Glaser S,Koslowski T,Friedrich T,Bechthold A
JournalThe Journal of biological chemistry
PubMed ID16537546
Mutational analysis of a fatty acyl-coenzyme A synthetase signature motif identifies seven amino acid residues that modulate fatty acid substrate specificity.
AuthorsBlack PN,Zhang Q,Weimar JD,DiRusso CC
JournalThe Journal of biological chemistry
PubMed ID9030548
The neuronal actin-binding proteins, neurabin I and neurabin II, recruit specific isoforms of protein phosphatase-1 catalytic subunits.
AuthorsTerry-Lorenzo Ryan T; Carmody Leigh C; Voltz James W; Connor John H; Li Shi; Smith F Donelson; Milgram Sharon L; Colbran Roger J; Shenolikar Shirish;
JournalJ Biol Chem
PubMed ID12016225
Neurabins are protein phosphatase-1 (PP1) targeting subunits that are highly concentrated in dendritic spines and post-synaptic densities. Immunoprecipitation of neurabin I and neurabin II/spinophilin from rat brain extracts sedimented PP1gamma1 and PP1alpha but not PP1beta. In vitro studies showed that recombinant peptides representing central regions of neurabins also preferentially bound ... More
Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis.
Authors Fazi Barbara; Cope M Jamie T V; Douangamath Alice; Ferracuti Silvia; Schirwitz Katja; Zucconi Adriana; Drubin David G; Wilmanns Matthias; Cesareni Gianni; Castagnoli Luisa;
JournalJ Biol Chem
PubMed ID11668184
'Abp1p is an actin-binding protein that plays a central role in the organization of Saccharomyces cerevisiae actin cytoskeleton. By a combination of two-hybrid and phage-display approaches, we have identified six new ligands of the Abp1-SH3 domain. None of these SH3-mediated novel interactions was detected in recent all genome high throughput ... More
Drosophila Sir2 Is Required for Heterochromatic Silencing and by Euchromatic Hairy/E(Spl) bHLH Repressors in Segmentation and Sex Determination.
Authors Rosenberg Miriam I; Parkhurst Susan M;
JournalCell
PubMed ID12086602
'Yeast SIR2 is a NAD(+)-dependent histone deacetylase required for heterochromatic silencing at telomeres, rDNA, and mating-type loci. We find that the Drosophila homolog of Sir2 (dSir2) also encodes deacetylase activity and is required for heterochromatic silencing, but unlike ySir2, is not required for silencing at telomeres. We show that dSir2 ... More
Shc and Enigma are both required for mitogenic signaling by Ret/ptc2.
AuthorsDurick K, Gill GN, Taylor SS
JournalMol Cell Biol
PubMed ID9528800
'Ret/ptc2 is a constitutively active, oncogenic form of the c-Ret receptor tyrosine kinase. Like the other papillary thyroid carcinoma forms of Ret, Ret/ptc2 is activated through fusion of the Ret tyrosine kinase domain to the dimerization domain of another protein. Investigation of requirements for Ret/ptc2 mitogenic activity, using coexpression with ... More
NF-Y is associated with the histone acetyltransferases GCN5 and P/CAF.
AuthorsCurrie RA
JournalJ Biol Chem
PubMed ID9430679
'The ubiquitous transcription factor, NF-Y, plays a pivotal role in the cell cycle regulation of the mammalian cyclin A, cdc25C, and cdc2 genes, in the S-phase activation of the ribonucleotide reductase R2 gene, in addition to its critical role as a key proximal promoter factor in the transcriptional regulation of ... More
T3 receptor suppression of Sp1-dependent transcription from the epidermal growth factor receptor promoter via overlapping DNA-binding sites.
AuthorsXu J, Thompson KL, Shephard LB, Hudson LG, Gill GN
JournalJ Biol Chem
PubMed ID8393457
'Expression of the human epidermal growth factor receptor (EGFR) gene is inhibited by ligand-activated thyroid hormone receptor (T3R). Binding sites for Sp1 and for the T3R.retinoid X receptor (RXR) complex overlap in a functional core of the EGFR promoter. Sp1 inhibited binding of the T3R complex to this 36-base pair ... More
Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing.
AuthorsHendrix P, Mayer-Jackel RE, Cron P, Goris J, Hofsteenge J, Merlevede W, Hemmings BA
JournalJ Biol Chem
PubMed ID8392071
'The trimeric form of protein phosphatase 2A consisting of 36-, 65-, and 72-kDa subunits (previously termed polycation-stimulated protein phosphatase M) was purified from rabbit skeletal muscle. Amino acid sequence data of the 72-kDa regulatory subunit (termed PR72) were used to isolate cDNAs from human heart and fetal brain libraries and ... More
Identification of residues in the single-stranded DNA-binding site of the 8-kDa domain of rat DNA polymerase beta by UV cross-linking.
AuthorsPrasad R, Kumar A, Widen SG, Casas-Finet JR, Wilson SH
JournalJ Biol Chem
PubMed ID8226785
'Rat DNA polymerase beta (beta-pol) is a 39-kDa monomeric protein, organized in two structurally and functionally distinct domains. The 8- kDa NH2-terminal domain binds single-stranded (ss) DNA, whereas the 31- kDa COOH-terminal domain does not. To facilitate studies on ssDNA binding structure-function relationships of beta-pol, we overexpressed the 8-kDa domain ... More
Depolarization drives beta-Catenin into neuronal spines promoting changes in synaptic structure and function.
Authors Murase Sachiko; Mosser Eric; Schuman Erin M;
JournalNeuron
PubMed ID12123611
'Activity-induced changes in adhesion molecules may coordinate presynaptic and postsynaptic plasticity. Here, we demonstrate that beta-catenin, which mediates interactions between cadherins and the actin cytoskeleton, moves from dendritic shafts into spines upon depolarization, increasing its association with cadherins. beta-catenin''s redistribution was mimicked or prevented by a tyrosine kinase or phosphatase ... More
The transcription factors GATA4 and dHAND physically interact to synergistically activate cardiac gene expression through a p300-dependent mechanism.
Authors Dai Yan-Shan; Cserjesi Peter; Markham Bruce E; Molkentin Jeffery D;
JournalJ Biol Chem
PubMed ID11994297
'An intricate array of heterogeneous transcription factors participate in programming tissue-specific gene expression through combinatorial interactions that are unique to a given cell-type. The zinc finger-containing transcription factor GATA4, which is widely expressed in mesodermal and endodermal derived tissues, is thought to regulate cardiac myocyte-specific gene expression through combinatorial interactions ... More
Structural basis for antagonist-mediated recruitment of nuclear co-repressors by PPARalpha.
Authors Xu H Eric; Stanley Thomas B; Montana Valerie G; Lambert Millard H; Shearer Barry G; Cobb Jeffery E; McKee David D; Galardi Cristin M; Plunket Kelli D; Nolte Robert T; Parks Derek J; Moore John T; Kliewer Steven A; Willson Timothy M; Stimmel Julie B;
JournalNature
PubMed ID11845213
'Repression of gene transcription by nuclear receptors is mediated by interactions with co-repressor proteins such as SMRT and N-CoR, which in turn recruit histone deacetylases to the chromatin. Aberrant interactions between nuclear receptors and co-repressors contribute towards acute promyelocytic leukaemia and thyroid hormone resistance syndrome. The binding of co-repressors to ... More
DNA Polymerase lambda, a Novel DNA Repair Enzyme in Human Cells.
Authors Garcia-Diaz Miguel; Bebenek Katarzyna; Sabariegos Rosario; Dominguez Orlando; Rodriguez Josana; Kirchhoff Tomas; Garcia-Palomero Esther; Picher Angel J; Juarez Raquel; Ruiz Jose F; Kunkel Thomas A; Blanco Luis;
JournalJ Biol Chem
PubMed ID11821417
'DNA polymerase lambda (pol lambda) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5''-deoxyribose-5-phosphate lyase activity in pol lambda supports a function of this enzyme in base excision repair. However, the ... More
A human sequence homologue of Staufen is an RNA-binding protein that is associated with polysomes and localizes to the rough endoplasmic reticulum.
AuthorsMarion RM, Fortes P, Beloso A, Dotti C, Ortin J
JournalMol Cell Biol
PubMed ID10022908
'In the course of a two-hybrid screen with the NS1 protein of influenza virus, a human clone capable of coding for a protein with high homology to the Staufen protein from Drosophila melanogaster (dmStaufen) was identified. With these sequences used as a probe, cDNAs were isolated from a lambda cDNA ... More
Nopp140 is a mediator of the protein kinase A signaling pathway that activates the acute phase response alpha1-acid glycoprotein gene.
Authors Chiu Chi-Ming; Tsay Yeou-Guang; Chang Ching-Jin; Lee Sheng-Chung;
JournalJ Biol Chem
PubMed ID12167624
'The acute phase response (APR) in liver during inflammation is one of the well known examples for elucidating the signaling pathways that lead to the combinatorial regulation of gene expression. The APR is exemplified by alpha(1)-acid glycoprotein gene (agp) expression. A number of transcription factors, including CCAAT/enhancer-binding protein beta (C/EBPbeta), ... More
XCTK2: a kinesin-related protein that promotes mitotic spindle assembly in Xenopus laevis egg extracts.
AuthorsWalczak CE, Verma S, Mitchison TJ
JournalJ Cell Biol
PubMed ID9049251
'We used a peptide antibody to a conserved sequence in the motor domain of kinesins to screen a Xenopus ovary cDNA expression library. Among the clones isolated were two that encoded a protein we named XCTK2 for Xenopus COOH-terminal kinesin 2. XCTK2 contains an NH2-terminal globular domain, a central alpha-helical ... More
The substrate binding domain of DnaK facilitates slow protein refolding.
Authors Tanaka Naoki; Nakao Shota; Wadai Hiromasa; Ikeda Shoichi; Chatellier Jean; Kunugi Shigeru;
JournalProc Natl Acad Sci U S A
PubMed ID12434017
'We examined the effects of a fragment of the substrate binding domain of DnaK on protein refolding from chemically denatured states. The fragment DnaK384-638, containing a full-length substrate binding domain, tightly binds to the unfolded protein in solution. The effects of DnaK384-638 on the reactivation of beta-galactosidase and luciferase were ... More
MIX-1: an essential component of the C. elegans mitotic machinery executes X chromosome dosage compensation.
AuthorsLieb JD, Albrecht MR, Chuang PT, Meyer BJ
JournalCell
PubMed ID9458050
'We show that a functional component of the C. elegans mitotic machinery regulates X chromosome gene expression. This protein, MIX-1, is a member of the dosage compensation complex that associates specifically with hermaphrodite X chromosomes to reduce their gene expression during interphase. MIX-1 also associates with all mitotic chromosomes to ... More
Expression of alpha2,8/2,9-polysialyltransferase from escherichia coli K92. Characterization Of the enzyme and its reaction products.
AuthorsShen GJ, Datta AK, Izumi M, Koeller KM, Wong CH
JournalJ Biol Chem
PubMed ID10574996
'The capsular polysaccharide of Escherichia coli K92 contains alternating -8-NeuAcalpha2- and -9-NeuAcalpha2- linkages. The enzyme catalyzing this polymerizing reaction has been cloned from the genomic DNA of E. coli K92. The 1.2-kilobase polymerase chain reaction fragment was subcloned in pRSET vector and the protein was expressed in the BL21(DE3) strain ... More
p70 lupus autoantigen binds the enhancer of the T-cell receptor beta- chain gene.
AuthorsMessier H, Fuller T, Mangal S, Brickner H, Igarashi S, Gaikwad J, Fotedar R, Fotedar A
JournalProc Natl Acad Sci U S A
PubMed ID8464876
'The p70 (Ku) autoantigen has been described as a nonhistone nuclear protein recognized by antibodies from lupus patients. In our studies on the regulation of T-cell receptor (TCR) beta-chain gene expression we have identified the p70 lupus autoantigen as a DNA-binding protein that binds the enhancer of the TCR beta-chain ... More
Bacillus subtilis sigma B is regulated by a binding protein (RsbW) that blocks its association with core RNA polymerase.
AuthorsBenson AK, Haldenwang WG
JournalProc Natl Acad Sci U S A
PubMed ID8460143
'sigma B is a secondary sigma factor of Bacillus subtilis. RNA polymerase containing sigma B transcribes a subset of genes that are expressed after heat shock or the onset of the stationary phase of growth. Three genes (rsbV, rsbW, and rsbX), cotranscribed with the sigma B structural gene (sigB), regulate ... More
A 127 kDa component of a UV-damaged DNA-binding complex, which is defective in some xeroderma pigmentosum group E patients, is homologous to a slime mold protein.
AuthorsTakao M, Abramic M, Moos M Jr, Otrin VR, Wootton JC, McLenigan M, Levine AS, Protic M
JournalNucleic Acids Res
PubMed ID8371985
'A cDNA which encodes a approximately 127 kDa UV-damaged DNA-binding (UV- DDB) protein with high affinity for (6-4)pyrimidine dimers [Abramic'', M., Levine, A.S. & Protic'', M., J. Biol. Chem. 266: 22493-22500, 1991] has been isolated from a monkey cell cDNA library. The presence of this protein in complexes bound to ... More
Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells.
AuthorsZhu J, Winans SC
JournalProc Natl Acad Sci U S A
PubMed ID10220379
'TraR is an Agrobacterium transcriptional regulator whose activity requires the pheromone N-3-oxooctanoyl-L-homoserine lactone. TraR was purified as a complex with the pheromone and contained one pheromone molecule per protein monomer. TraR-pheromone complexes bound to a single DNA site and activated two promoters that flank this site. Promoter expression was elevated ... More
Role of phosphoinositide 3-OH kinase in cell transformation and control of the actin cytoskeleton by Ras.
AuthorsRodriguez-Viciana P, Warne PH, Khwaja A, Marte BM, Pappin D, Das P, Waterfield MD, Ridley A, Downward J
JournalCell
PubMed ID9150145
'The pathways by which mammalian Ras proteins induce cortical actin rearrangement and cause cellular transformation are investigated using partial loss of function mutants of Ras and activated and inhibitory forms of various postulated target enzymes for Ras. Efficient transformation by Ras requires activation of other direct effectors in addition to ... More
Bone morphogenetic protein-1: the type I procollagen C-proteinase [see comments]
AuthorsKessler E, Takahara K, Biniaminov L, Brusel M, Greenspan DS
JournalScience
PubMed ID8553073
'Bone morphogenetic proteins (BMPs) are bone-derived factors capable of inducing ectopic bone formation. Unlike other BMPs, BMP-1 is not like transforming growth factor-beta (TGF-beta), but it is the prototype of a family of putative proteases implicated in pattern formation during development in diverse organisms. Although some members of this group, ... More
Identification and characterization of two cation binding sites in the integrin beta 3 subunit.
Authors Cierniewska-Cieslak Aleksandra; Cierniewski Czeslaw S; Blecka Kamila; Papierak Malgorzata; Michalec Lidia; Zhang Li; Haas Thomas A; Plow Edward F;
JournalJ Biol Chem
PubMed ID11796735
'The midsegment of the beta(3) subunit has been implicated in the ligand and cation binding functions of the beta(3) integrins. This region may contain a metal ion-dependent adhesion site (MIDAS) and fold into an I domain-like structure. Two recombinant fragments, beta(3)-(95-373) and beta(3)-(95-301), were expressed and found to bind fibrinogen. ... More
Three-dimensional solution structure and 13C assignments of barstar using nuclear magnetic resonance spectroscopy.
AuthorsLubienski MJ, Bycroft M, Freund SM, Fersht AR
JournalBiochemistry
PubMed ID8043574
'We present the high-resolution solution structure and 13C assignments of wild-type barstar, an 89 amino acid residue polypeptide inhibitor of barnase, derived from heteronuclear NMR techniques. These were obtained from measurements on unlabeled, uniformly 15N- and 13C/15N-labeled, and 10% 13C-labeled barstar samples that have both cysteines (at positions 40 and ... More
Mapping of Ras-related GTP-binding proteins by GTP overlay following two-dimensional gel electrophoresis.
AuthorsHuber LA, Ullrich O, Takai Y, Lutcke A, Dupree P, Olkkonen V, Virta H, de Hoop MJ, Alexandrov K, Peter M, et al
JournalProc Natl Acad Sci U S A
PubMed ID8058727
'For identification of Rab, Rac, Rho, Ral, Rap, and Arf proteins on two- dimensional polyacrylamide gels, we have expressed full-length cDNAs of members of these protein families with the T7 RNA polymerase- recombinant vaccinia virus expression system. Membrane preparations from cells expressing the cDNAs were subjected to high-resolution two- dimensional ... More
Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans.
AuthorsLuckow B, Bunz F, Stillman B, Lichter P, Schutz G
JournalMol Cell Biol
PubMed ID8114700
'We have isolated a full-length mouse cDNA encoding a lysine-rich protein of 1,131 amino acids with a calculated molecular mass of 126 kDa. The protein binds in a sequence-unspecific manner to DNA, is localized exclusively in the nucleus, and contains a putative ATP binding site and a stretch of 80 ... More
Interaction of SAP97 with minus-end-directed actin motor myosin VI. Implications for AMPA receptor trafficking.
Authors Wu Hongju; Nash Joanne E; Zamorano Pedro; Garner Craig C;
JournalJ Biol Chem
PubMed ID12050163
'SAP97 is a modular protein composed of three PDZ domains, an SH3 domain, and a guanylate kinase-like domain. It has been implicated functionally in the assembly and structural stability of synaptic junctions as well as in the trafficking, recruitment, and localization of specific ion channels and neurotransmitter receptors. The N ... More
A role for the M9 transport signal of hnRNP A1 in mRNA nuclear export.
AuthorsIzaurralde E, Jarmolowski A, Beisel C, Mattaj IW, Dreyfuss G, Fischer U
JournalJ Cell Biol
PubMed ID9105034
'Among the nuclear proteins associated with mRNAs before their export to the cytoplasm are the abundant heterogeneous nuclear (hn) RNPs. Several of these contain the M9 signal that, in the case of hnRNP A1, has been shown to be sufficient to signal both nuclear export and nuclear import in cultured ... More
Identification of glycerol-3-phosphate acyltransferase as an adipocyte determination and differentiation factor 1- and sterol regulatory element-binding protein-responsive gene.
AuthorsEricsson J, Jackson SM, Kim JB, Spiegelman BM, Edwards PA
JournalJ Biol Chem
PubMed ID9054427
'We demonstrate that the mRNA levels of glycerol-3-phosphate acyltransferase (GPAT), a mitochondrial enzyme catalyzing the initial step in glycerolipid synthesis, are induced during the differentiation of 3T3-L1 preadipocytes to adipocytes and following ectopic expression of rat adipocyte determination and differentiation factor 1 (ADD1), a protein with high homology to the ... More
Combinatorial interactions of p53, activating protein-2, and YB-1 with a single enhancer element regulate gelatinase A expression in neoplastic cells.
Authors Mertens Peter R; Steinmann Karin; Alfonso-Jaume Maria A; En-Nia Abdelaziz; Sun Yi; Lovett David H;
JournalJ Biol Chem
PubMed ID11973333
'Gelatinase A, also denoted matrix metalloproteinase 2, plays multiple critical roles in the neoplastic process, including facilitation of neoangiogenesis and formation of distal metastases. The transcriptional regulation of the gelatinase A gene is under the control of strong, evolutionarily conserved cis-acting enhancer elements, designated the r2 (human) or RE-1 (rat), ... More
Shank2 associates with and regulates Na+/H+ exchanger 3.
AuthorsHan W, Kim KH, Jo MJ, Lee JH, Yang J, Doctor RB, Moe OW, Lee J, Kim E, Lee MG,
JournalJ Biol Chem
PubMed ID16293618
'Na+/H+ exchanger 3 (NHE3) plays a pivotal role in transepithelial Na+ and HCO3(-) absorption across a wide range of epithelia in the digestive and renal-genitourinary systems. Accumulating evidence suggests that PDZ-based adaptor proteins play an important role in regulating the trafficking and activity of NHE3. A search for NHE3-binding modular ... More
Pseudomonas aeruginosa aspartate transcarbamoylase. Characterization of its catalytic and regulatory properties.
Authors Vickrey John F; Herve Guy; Evans David R;
JournalJ Biol Chem
PubMed ID11959858
'Aspartate transcarbamoylase from Pseudomonadaceae is a class A enzyme consisting of six copies of a 36-kDa catalytic chain and six copies of a 45-kDa polypeptide of unknown function. The 45-kDa polypeptide is homologous to dihydroorotase but lacks catalytic activity. Pseudomonas aeruginosa aspartate transcarbamoylase was overexpressed in Escherichia coli. The homogeneous ... More
Controlling Gene Expression in Living Cells Through Small Molecule-RNA Interactions
AuthorsGeoffrey Werstuck and Michael R. Green
JournalScience
PubMed ID9765156
'Short RNA aptamers that specifically bind to a wide variety of ligandsin vitro can be isolated from randomized pools of RNA. Here it isshown that small molecule aptamers also bound their ligand in vivo,enabling development of a method for controlling gene expression inliving cells. Insertion of a small molecule aptamer ... More
A defect in the nuclear translocation of CIITA causes a form of type II bare lymphocyte syndrome.
AuthorsCressman DE, Chin KC, Taxman DJ, Ting JP
JournalImmunity
PubMed ID10072069
'The severe immunodeficiency type II bare lymphocyte syndrome (BLS) lacks class II MHC gene transcription. One defect from a complementation group A type II BLS patient is a 24 aa deletion in the MHC class II transactivator (CIITA). We show here that the molecular defect present in this protein is ... More
Dosage Compensation Proteins Targeted to X Chromosomes by a Determinant of Hermaphrodite Fate
AuthorsHeather E. Dawes, Dorit S. Berlin, Denise M. Lapidus, Chad Nusbaum, Tamara L. Davis, and Barbara J. Meyer
JournalScience
PubMed ID10364546
'In many organisms, master control genes coordinately regulatesex-specific aspects of development. SDC-2 was shown toinduce hermaphrodite sexual differentiation and activate Xchromosome dosage compensation in Caenorhabditiselegans. To control these distinct processes, SDC-2 acts as astrong gene-specific repressor and a weakerchromosome-wide repressor. To initiate hermaphrodite development, SDC-2 associates with the promoterof the ... More
A bipolar kinesin.
AuthorsKashina AS, Baskin RJ, Cole DG, Wedaman KP, Saxton WM, Scholey JM
JournalNature
PubMed ID8538794
'Chromosome segregation during mitosis depends on the action of the mitotic spindle, a self-organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors. Members of the BimC subfamily of kinesin-related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle. Here ... More
DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2.
AuthorsShieh SY, Ikeda M, Taya Y, Prives C
JournalCell
PubMed ID9363941
'DNA-damaging agents signal to p53 through as yet unidentified posttranscriptional mechanisms. Here we show that phosphorylation of human p53 at serine 15 occurs after DNA damage and that this leads to reduced interaction of p53 with its negative regulator, the oncoprotein MDM2, in vivo and in vitro. Furthermore, using purified ... More
Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression.
AuthorsChang CJ, Chen YL, Lee SC
JournalMol Cell Biol
PubMed ID9742105
'The transcription of the alpha1-acid glycoprotein gene is induced by inflammatory cytokines and glucocorticoids. C/EBPbeta is a major transcription factor involved in the induction of the agp gene by some cytokines. In this report, we have identified a novel transcriptional intermediary factor, TIF1beta, which could enhance the transcription of the ... More
Characterization of the DNA-binding and dimerization properties of the nuclear orphan receptor germ cell nuclear factor.
AuthorsGreschik H, Wurtz JM, Hublitz P, Kohler F, Moras D, Schule R
JournalMol Cell Biol
PubMed ID9858592
'The orphan receptor germ cell nuclear factor (GCNF) is a member of the superfamily of nuclear receptors. During development, GCNF exhibits a restricted brain-specific expression pattern, whereas GCNF expression in the adult is germ cell specific. Therefore, the receptor may participate in the regulation of neurogenesis and reproductive functions. No ... More
Two-color GFP expression system for C. elegans.
AuthorsMiller DM 3rd, Desai NS, Hardin DC, Piston DW, Patterson GH, Fleenor J, Xu S, Fire A
JournalBiotechniques
PubMed ID10337485
We describe the use of modified versions of the Aequora victoria green fluorescent protein (GFP) to simultaneously follow the expression and distribution of two different proteins in the nematode, Caenorhabditis elegans. A cyan-colored GFP derivative, designated CFP, contains amino acid (aa) substitutions Y66W, N146I, M153T and V163A relative to the ... More
Evolutionarily Conserved Pathways of Energetic Connectivity in Protein Families
AuthorsSteve W. Lockless, Rama Ranganathan *
JournalScience
PubMed ID10514373
For mapping energetic interactions in proteins, a technique was developed that uses evolutionary data for a protein family to measure statistical interactions between amino acid positions. Forthe PDZ domain family, this analysis predicted a set of energetically coupled positions for a binding site residue that includes unexpected long-range interactions. Mutational ... More
A new splice variant of glial fibrillary acidic protein, GFAP epsilon, interacts with the presenilin proteins.
AuthorsNielsen AL, Holm IE, Johansen M, Bonven B, Jorgensen P, Jorgensen AL.
JournalJ Biol Chem
PubMed ID12058025
We describe a new human isoform, GFAP epsilon, of the intermediary filament protein GFAP (glial fibrillary acidic protein). GFAP epsilon mRNA is the result of alternative splicing and a new polyadenylation signal, and thus GFAP epsilon has a new C-terminal protein sequence. This provides GFAP epsilon with the capacity for ... More
Direct Targeting of Light Signals to aPromoter Element-Bound Transcription Factor
AuthorsJaime F. MartÝnez-GarcÝa, Enamul Huq, Peter H. Quail *
JournalScience
PubMed ID10797009
Light signals perceived by the phytochrome family of sensoryphotoreceptors are transduced to photoresponsive genes by an unknownmechanism. Here, we show that the basic helix-loop-helix transcriptionfactor PIF3 binds specifically to a G-box DNA-sequence motif present invarious light-regulated gene promoters, and that phytochrome B bindsreversibly to G-box-bound PIF3 specifically upon light-triggeredconversion of ... More
A direct interaction between the survival motor neuron protein and p53 and its relationship to spinal muscular atrophy.
Authors Young Philip J; Day Patricia M; Zhou Jianhua; Androphy Elliot J; Morris Glenn E; Lorson Christian L;
JournalJ Biol Chem
PubMed ID11704667
Mutations in the SMN1 (survival motor neuron 1) gene cause spinal muscular atrophy (SMA). We now show that SMN protein, the SMN1 gene product, interacts directly with the tumor suppressor protein, p53. Pathogenic missense mutations in SMN reduce both self-association and p53 binding by SMN, and the extent of the ... More
Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase [see comments]
AuthorsKamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG Jr, Elledge SJ, Conaway RC, Harper JW, Conaway JW
JournalScience
PubMed ID10213691
The von Hippel-Lindau (VHL) tumor suppressor gene is mutated in most human kidney cancers. The VHL protein is part of a complex that includes Elongin B, Elongin C, and Cullin-2, proteins associated with transcriptional elongation and ubiquitination. Here it is shown that the endogenous VHL complex in rat liver also ... More
The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases.
AuthorsMitchell P, Petfalski E, Shevchenko A, Mann M, Tollervey D
JournalCell
PubMed ID9390555
We identified a complex in S. cerevisiae, the exosome, consisting of the five essential proteins Rrp4p, Rrp41p, Rrp42p, Rrp43p, and Rrp44p (Dis3p). Remarkably, four of these proteins are homologous to characterized bacterial 3'-->5' exoribonucleases; Rrp44p is homologous to RNase II, while Rrp41p, Rrp42p, and Rrp43p are related to RNase PH. ... More
HTLV-I Tax protein stimulation of DNA binding of bZIP proteins by enhancing dimerization.
AuthorsWagner S, Green MR
JournalScience
PubMed ID8211160
The Tax protein of human T cell leukemia virus type-1 (HTLV-I) transcriptionally activates the HTLV-I promoter. This activation requires binding sites for activating transcription factor (ATF) proteins, a family of cellular proteins that contain basic region- leucine zipper (bZIP) DNA binding domains. Data are presented showing that Tax increases the ... More
CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment.
AuthorsWood KW, Sakowicz R, Goldstein LS, Cleveland DW
JournalCell
PubMed ID9363944
Mitosis requires dynamic attachment of chromosomes to spindle microtubules. This interaction is mediated largely by kinetochores. During prometaphase, forces exerted at kinetochores, in combination with polar ejection forces, drive congression of chromosomes to the metaphase plate. A major question has been whether kinetochore- associated microtubule motors play an important role ... More
A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation.
Authors Delaunay Agnès; Pflieger Delphine; Barrault Marie Bénédicte; Vinh Joelle; Toledano Michel B;
JournalCell
PubMed ID12437921
The Yap1 transcription factor regulates hydroperoxide homeostasis in S. cerevisiae. Yap1 is activated by oxidation when hydroperoxide levels increase. We show that Yap1 is not directly oxidized by hydroperoxide. We identified the glutathione peroxidase (GPx)-like enzyme Gpx3 as a second component of the pathway, serving the role of sensor and ... More
The neuron-restrictive silencer factor (NRSF): a coordinate repressor of multiple neuron-specific genes.
AuthorsSchoenherr CJ, Anderson DJ
JournalScience
PubMed ID7871435
The neuron-restrictive silencer factor (NRSF) binds a DNA sequence element, called the neuron-restrictive silencer element (NRSE), that represses neuronal gene transcription in nonneuronal cells. Consensus NRSEs have been identified in 18 neuron-specific genes. Complementary DNA clones encoding a functional fragment of NRSF were isolated and found to encode a novel ... More
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts.
Authors Kalab Petr; Weis Karsten; Heald Rebecca;
JournalScience
PubMed ID11923538
The small guanosine triphosphatase Ran is loaded with guanosine triphosphate (GTP) by the chromatin-bound guanine nucleotide exchange factor RCC1 and releases import cargoes in the nucleus during interphase. In mitosis, Ran-GTP promotes spindle assembly around chromosomes by locally discharging cargoes that regulate microtubule dynamics and organization. We used fluorescence resonance ... More
Phosphorylation of TFIIA Stimulates TATA Binding Protein-TATA Interaction and Contributes to Maximal Transcriptionand Viability in Yeast
AuthorsSolow SP, Lezina L, Lieberman PM
JournalMol Cell Biol
PubMed ID10082550
Posttranslational modification of general transcription factors may be an important mechanism for globalgene regulation. The general transcription factor IIA (TFIIA) binds to the TATA binding protein (TBP) andis essential for high-level transcription mediated by various activators. Modulation of the TFIIA-TBP inter-actionis a likely target of transcriptional regulation. We report here ... More
Schwannomin inhibits tumorigenesis through direct interaction with the eukaryotic initiation factor subunit c (eIF3c).
AuthorsScoles DR, Yong WH, Qin Y, Wawrowsky K, Pulst SM,
JournalHum Mol Genet
PubMed ID16497727
The neurofibromatosis 2 (NF2) tumor suppressor protein, schwannomin or merlin, is commonly lost upon NF2 gene mutation in benign human brain tumors. We identified the p110 subunit of the eukaryotic initiation factor 3 (eIF3c) as a schwannomin interacting protein. The eIF3 complex consists of approximately 10 subunits whose functions are ... More
A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus.
AuthorsYeh PY, Kuo SH, Yeh KH, Chuang SE, Hsu CH, Chang WC, Lin HI, Gao M, Cheng AL,
JournalJ Biol Chem
PubMed ID16280327
Bcl10 overexpression and nuclear translocation were originally identified in mucosa-associated lymphoid tissue lymphoma with t(1;14)(p32;q32) chromosome translocation. DNA amplification of Bcl10 was also found in other solid tumors. We have recently shown that nuclear translocation of Bcl10 is a specific molecular determinant of Helicobacter pylori-independent mucosa-associated lymphoid tissue lymphoma (Kuo, ... More
Identification and characterization of mitochondrial abasic (AP)-endonuclease in mammalian cells.
AuthorsChattopadhyay R, Wiederhold L, Szczesny B, Boldogh I, Hazra TK, Izumi T, Mitra S,
JournalNucleic Acids Res
PubMed ID16617147
Abasic (AP)-endonuclease (APE) is responsible for repair of AP sites, and single-strand DNA breaks with 3' blocking groups that are generated either spontaneously or during repair of damaged or abnormal bases via the DNA base excision repair (BER) pathway in both nucleus and mitochondria. Mammalian cells express only one nuclear ... More
Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light.
AuthorsNi M, Tepperman JM, Quail PH
JournalNature
PubMed ID10466729
The phytochrome photoreceptor family directs plant gene expression by switching between biologically inactive and active conformers in response to the sequential absorption of red and farred photons. Several intermediates that act late in the phytochrome signalling pathway have been identified, but fewer have been identified that act early in the ... More
A cytoplasmic dynein heavy chain is required for oscillatory nuclear movement of meiotic prophase and efficient meiotic recombination in fission yeast.
AuthorsYamamoto A, West RR, McIntosh JR, Hiraoka Y
JournalJ Cell Biol
PubMed ID10366596
Meiotic recombination requires pairing of homologous chromosomes, the mechanisms of which remain largely unknown. When pairing occurs during meiotic prophase in fission yeast, the nucleus oscillates between the cell poles driven by astral microtubules. During these oscillations, the telomeres are clustered at the spindle pole body (SPB), located at the ... More
Subcellular localization of Bacillus subtilis SMC, a protein involved in chromosome condensation and segregation.
AuthorsGraumann PL, Losick R, Strunnikov AV
JournalJ Bacteriol
PubMed ID9791128
We have investigated the subcellular localization of the SMC protein in the gram-positive bacterium Bacillus subtilis. Recent work has shown that SMC is required for chromosome condensation and faithful chromosome segregation during the B. subtilis cell cycle. Using antibodies against SMC and fluorescence microscopy, we have shown that SMC is ... More
Ligand binding and co-activator assembly of the peroxisome proliferator- activated receptor-gamma.
AuthorsNolte RT, Wisely GB, Westin S, Cobb JE, Lambert MH, Kurokawa R, Rosenfeld MG, Willson TM, Glass CK, Milburn MV
JournalNature
PubMed ID9744270
The peroxisome proliferator-activated receptor-gamma (PPAR-gamma) is a ligand-dependent transcription factor that is important in adipocyte differentiation and glucose homeostasis and which depends on interactions with co-activators, including steroid receptor co- activating factor-1 (SRC-1). Here we present the X-ray crystal structure of the human apo-PPAR-gamma ligand-binding domain (LBD), at 2.2 A ... More
The Caenorhabditis elegans gene ham-2 links Hox patterning to migration of the HSN motor neuron.
AuthorsBaum PD, Guenther C, Frank CA, Pham BV, Garriga G
JournalGenes Dev
PubMed ID10049362
The Caenorhabditis elegans HSN motor neurons permit genetic analysis of neuronal development at single-cell resolution. The egl-5 Hox gene, which patterns the posterior of the embryo, is required for both early (embryonic) and late (larval) development of the HSN. Here we show that ham-2 encodes a zinc finger protein that ... More
Esa1p is an essential histone acetyltransferase required for cell cycle progression.
AuthorsClarke AS, Lowell JE, Jacobson SJ, Pillus L
JournalMol Cell Biol
PubMed ID10082517
Histones are dynamically modified during chromatin assembly, as specific transcriptional patterns are established, and during mitosis and development. Modifications include acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation, but the biological significance of each of these is not well understood. For example, distinct acetylation patterns correlate with nucleosome formation and with transcriptionally ... More
An orphan nuclear receptor activated by pregnanes defines a novel steroid signaling pathway.
AuthorsKliewer SA, Moore JT, Wade L, Staudinger JL, Watson MA, Jones SA, McKee DD, Oliver BB, Willson TM, Zetterstrom RH, Perlmann T, Lehmann JM
JournalCell
PubMed ID9489701
Steroid hormones exert profound effects on differentiation, development, and homeostasis in higher eukaryotes through interactions with nuclear receptors. We describe a novel orphan nuclear receptor, termed the pregnane X receptor (PXR), that is activated by naturally occurring steroids such as pregnenolone and progesterone, and synthetic glucocorticoids and antiglucocorticoids. PXR exists ... More
On/off blinking and switching behaviour of single molecules of green fluorescent protein.
AuthorsDickson RM, Cubitt AB, Tsien RY, Moerner WE
JournalNature
PubMed ID9237752
Optical studies of individual molecules at low and room temperature can provide information about the dynamics of local environments in solids, liquids and biological systems unobscured by ensemble averaging. Here we present a study of the photophysical behaviour of single molecules of the green fluorescent protein (GFP) derived from the ... More
Assembly of a rod-shaped chimera of a trimeric GCN4 zipper and the HIV- 1 gp41 ectodomain expressed in Escherichia coli.
AuthorsWeissenhorn W, Calder LJ, Dessen A, Laue T, Skehel JJ, Wiley DC
JournalProc Natl Acad Sci U S A
PubMed ID9177169
The HIV-1 envelope subunit gp41 plays a role in viral entry by initiating fusion of the viral and cellular membranes. A chimeric molecule was constructed centered on the ectodomain of gp41 without the fusion peptide, with a trimeric isoleucine zipper derived from GCN4 (pIIGCN4) on the N terminus and part ... More
A diffusible cytotoxin of Haemophilus ducreyi.
AuthorsCope LD, Lumbley S, Latimer JL, Klesney-Tait J, Stevens MK, Johnson LS, Purven M, Munson RS Jr, Lagergard T, Radolf JD, Hansen EJ
JournalProc Natl Acad Sci U S A
PubMed ID9108104
Little is known about the virulence mechanisms employed by Haemophilus ducreyi in the production of genital ulcers. This Gram-negative bacterium previously has been shown to produce a soluble cytotoxic activity that kills HeLa and HEp-2 cells. We have now identified a cluster of three H. ducreyi genes that encode this ... More
Nuclear Protein Phosphatase 2A Dephosphorylates Protein Kinase A-Phosphorylated CREB and Regulated CREB Transcriptional Stimulation
AuthorsBrian E. Wadzinski , William H. Wheat , Stephen Jaspers , Leonard F. Peruski , Jr. , Ronald L. Lickteig , Gary L. Johnson , and Dwight J. Klemm
JournalMol Cell Biol
PubMed ID8386317
Cyclic AMP (cAMP)-dependent protein kinase A (PKA) stimulates the transcription of many eucaryotic genes by catalyzing the phosphorylation of the cAMP-regulatory element binding protein (CREB). Conversely, the attenuation or inhibition of cAMP-stimulated gene transcription would require the dephosphorylation of CREB by a nuclear protein phosphatase. In HepG2 cells treated with ... More
Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA.
AuthorsVerrijzer CP, Yokomori K, Chen JL, Tjian R
JournalScience
PubMed ID8178153
In Drosophila and human cells, the TATA binding protein (TBP) of the transcription factor IID (TFIID) complex is tightly associated with multiple subunits termed TBP-associated factors (TAFs) that are essential for mediating regulation of RNA polymerase II transcription. The Drosophila TAFII150 has now been molecularly cloned and biochemically characterized. The ... More
Isolation and characterization of a cDNA encoding rat liver cytosolic epoxide hydrolase and its functional expression in Escherichia coli.
AuthorsKnehr M, Thomas H, Arand M, Gebel T, Zeller HD, Oesch F
JournalJ Biol Chem
PubMed ID8349641
A cDNA of 1992 base pairs encoding the complete rat liver cytosolic epoxide hydrolase has been isolated using a polymerase chain reaction- derived DNA fragment (Arand, M., Knehr, M., Thomas, H., Zeller, H. D., and Oesch, F. (1991) FEBS Lett. 294, 19-22) known to represent the 3'- end of the ... More
Molecular expression of the alpha-chemokine rabbit GRO in Escherichia coli and characterization of its production by lung cells in vitro and in vivo.
AuthorsJohnson MC 2nd, Kajikawa O, Goodman RB, Wong VA, Mongovin SM, Wong WB, Fox-Dewhurst R, Martin TR
JournalJ Biol Chem
PubMed ID8631900
GRO proteins are alpha-chemokine cytokines that attract neutrophils and stimulate the growth of a variety of cells. Previously, we observed that rabbit alveolar macrophages transcribe the genes for at least two GRO homologues. In order to study the role of GRO cytokines in lung inflammation, we cloned the predominant rabbit ... More
The yeast Pan2 protein is required for poly(A)-binding protein- stimulated poly(A)-nuclease activity.
AuthorsBoeck R, Tarun S Jr, Rieger M, Deardorff JA, Muller-Auer S, Sachs AB
JournalJ Biol Chem
PubMed ID8550599
The removal of the mRNA poly(A) tail in the yeast Saccharomyces cerevisiae is stimulated by the poly(A)-binding protein (Pab1p). A large scale purification of the Pab1p-stimulated poly(A) ribonuclease (PAN) identifies a 76-kDa and two 135-Da polypeptides as candidate enzyme subunits. Antibodies against the Pan1p protein, which is the minor 135-kDa ... More
Simian virus 40 small tumor antigen inhibits dephosphorylation of protein kinase A-phosphorylated CREB and regulates CREB transcriptional stimulation.
AuthorsWheat WH, Roesler WJ, Klemm DJ
JournalMol Cell Biol
PubMed ID8065321
We report that the small tumor (small-t) antigen of simian virus 40 (SV40) forms complexes with nuclear protein phosphatase 2A (PP2A) and regulates the phosphorylation and transcriptional transactivation function of the cyclic AMP (cAMP)-regulatory element binding protein (CREB). PP2A coimmunoprecipitated with small t from nuclear extracts from HepG2 cells expressing ... More
Multiple forms of C/EBP beta bind the EFII enhancer sequence in the Rous sarcoma virus long terminal repeat [published erratum appears in Mol Cell Biol 1994 Aug;14(8):5617]
AuthorsSears RC, Sealy L
JournalMol Cell Biol
PubMed ID8007984
In this report we demonstrate that C/EBP beta is a major component of three EFII DNA binding complexes, EFIIa, EFIIb, and EFIIc, which we have previously shown to specifically recognize a C/EBP consensus binding site found in the EFII enhancer sequence from the Rous sarcoma virus long terminal repeat (R. ... More
A novel POU domain protein which binds to the T-cell receptor beta enhancer.
AuthorsMessier H, Brickner H, Gaikwad J, Fotedar A
JournalMol Cell Biol
PubMed ID8102789
POU domain proteins have been implicated in the regulation of a number of lineage-specific genes. Among the first POU domain proteins described were the immunoglobulin octamer-binding proteins Oct-1 and Oct-2. It was therefore of special interest when we identified a novel lymphoid POU domain protein in Southwestern (DNA-protein) screens of ... More
The eRF clone corresponds to tryptophanyl-tRNA synthetase, not mammalian release factor.
AuthorsTimchenko L, Caskey CT
JournalProc Natl Acad Sci U S A
PubMed ID8146190
To study the similarity between a putative cloned mammalian release factor (RF) and tryptophanyl-tRNA synthetase (TRS), a recombinant rabbit RF fusion protein was expressed from prokaryotic expression vectors. Purified fractions of the fusion proteins were tested for TRS and RF activities. Addition of the fusion protein to a TRS assay ... More
Control of transcription by Kruppel through interactions with TFIIB and TFIIE beta [see comments]
AuthorsSauer F, Fondell JD, Ohkuma Y, Roeder RG, Jackle H
JournalNature
PubMed ID7753175
The zinc-finger protein Kruppel (Kr) is an integral part of the Drosophila segmentation gene cascade and is essential in organogenesis during later embryonic development. In tissue culture, Kr regulates transcription. Monomeric Kr can act as a transcriptional activator, whereas Kr dimers formed at high concentrations cause repression. Here we show ... More
The product of hedgehog autoproteolytic cleavage active in local and long-range signalling.
AuthorsPorter JA, von Kessler DP, Ekker SC, Young KE, Lee JJ, Moses K, Beachy PA
JournalNature
PubMed ID7885476
The secreted protein products of the hedgehog (hh) gene family are associated with local and long-range signalling activities that are responsible for developmental patterning in multiple systems, including Drosophila embryonic and larval tissues and vertebrate neural tube, limbs and somites. In a process that is critical for full biological activity, ... More
DPY-27:a chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome [see comments]
AuthorsChuang PT, Albertson DG, Meyer BJ
JournalCell
PubMed ID7954812
dpy-27 is an essential dosage compensation gene that acts to reduce expression of both hermaphrodite X chromosomes. The DPY-27 protein becomes specifically localized to the X chromosomes of wild-type XX embryos, but remains diffusely distributed throughout the nuclei of male (XO) embryos. In xol-1 mutant XO embryos that activate the ... More
Characterization of the KLP68D kinesin-like protein in Drosophila: possible roles in axonal transport.
AuthorsPesavento PA, Stewart RJ, Goldstein LS
JournalJ Cell Biol
PubMed ID7525600
This paper describes the molecular and biochemical properties of KLP68D, a new kinesin-like motor protein in Drosophila melanogaster. Sequence analysis of a full-length cDNA encoding KLP68D demonstrates that this protein has a domain that shares significant sequence identity with the entire 340-amin acid kinesin heavy chain motor domain. Sequences extending ... More
Calmodulin-binding sites on adenylyl cyclase type VIII.
AuthorsGu C, Cooper DM
JournalJ Biol Chem
PubMed ID10075700
Ca2+ stimulation of adenylyl cyclase type VIII (ACVIII) occurs through loosely bound calmodulin. However, where calmodulin binds in ACVIII and how the binding activates this cyclase have not yet been investigated. We have located two putative calmodulin-binding sites in ACVIII. One site is located at the N terminus as revealed ... More
Positive selection vectors to generate fused genes for the expression of his-tagged proteins.
AuthorsVan Reeth T, Dreze PL, Szpirer J, Szpirer C, Gabant P
JournalBiotechniques
PubMed ID9821593
Epitope tagging simplifies detection, characterization and purification of proteins. Gene fusion to combine the coding region of a well- characterized epitope with the coding region for a protein of interest generally requires several subcloning steps. Alternatively, a PCR strategy can be used to generate such a chimeric gene. In addition ... More
The PERIANTHIA gene encodes a bZIP protein involved in the determination of floral organ number in Arabidopsis thaliana.
AuthorsChuang CF, Running MP, Williams RW, Meyerowitz EM
JournalGenes Dev
PubMed ID9990857
Mutations in the PERIANTHIA (PAN) gene of Arabidopsis thaliana specifically transform flowers from tetramerous to largely pentamerous, which is a characteristic of flowers of ancestral plants. We have cloned the PAN gene and here we show that it encodes a member of the basic region/leucine zipper class of transcription factors. ... More
Molecular cloning and characterization of PKC theta, a novel member of the protein kinase C (PKC) gene family expressed predominantly in hematopoietic cells.
AuthorsBaier G, Telford D, Giampa L, Coggeshall KM, Baier-Bitterlich G, Isakov N, Altman A
JournalJ Biol Chem
PubMed ID8444877
Members of the protein kinase C (PKC) family of serine/threonine kinases play a key role in regulating the differentiation and growth of diverse cell types and, to date, the cloning of seven mammalian PKC genes encoding eight distinct isoforms has been reported. Here we describe the molecular cloning and deduced ... More
Characterization of the NR1, NR2A, and NR2C receptor proteins.
AuthorsKopke AK, Bonk I, Sydow S, Menke H, Spiess J
JournalProtein Sci
PubMed ID8298456
N-Methyl-D-aspartate (NMDA) receptor subunits were characterized with seven polyclonal antibodies. The antibodies were directed against NR1- A, NR2A-N1, and NR2C-N1, representing N-terminal sequences of the NR1, NR2A, and NR2C subunits, and against NR1-E, NR2A-C1, and NR2C-C1, derived from C-terminal sequences of these subunits. The anti-NR1-D antibody was raised against the ... More
Asp333, Asp495, and His523 form the catalytic triad of rat soluble epoxide hydrolase.
AuthorsArand M, Wagner H, Oesch F
JournalJ Biol Chem
PubMed ID8626766
On the basis of the sequence similarity between mammalian epoxide hydrolases and bacterial haloalkane dehalogenase reported earlier (Arand, M., Grant, D. F., Beetham, J. K., Friedberg, T., Oesch, F., and Hammock, B. D. (1994) FEBS Lett. 338, 251-256; Beetham, J. K., Grant, D., Arand, M., Garbarino, J., Kiyosue, T., Pinot, ... More
GKAP, a novel synaptic protein that interacts with the guanylate kinase- like domain of the PSD-95/SAP90 family of channel clustering molecules.
AuthorsKim E, Naisbitt S, Hsueh YP, Rao A, Rothschild A, Craig AM, Sheng M
JournalJ Cell Biol
PubMed ID9024696
The molecular mechanisms underlying the organization of ion channels and signaling molecules at the synaptic junction are largely unknown. Recently, members of the PSD-95/SAP90 family of synaptic MAGUK (membrane-associated guanylate kinase) proteins have been shown to interact, via their NH2-terminal PDZ domains, with certain ion channels (NMDA receptors and K+ ... More
Characterization of the interaction between P143 and LEF-3 from two different baculovirus species: Choristoneura fumiferana nucleopolyhedrovirus LEF-3 can complement Autographa californica nucleopolyhedrovirus LEF-3 in supporting DNA replication.
AuthorsChen T, Sahri D, Carstens EB,
JournalJ Virol
PubMed ID14671115
The baculovirus protein P143 is essential for viral DNA replication in vivo, likely as a DNA helicase. We have demonstrated that another viral protein, LEF-3, first described as a single-stranded DNA binding protein, is required for transporting P143 into the nuclei of insect cells. Both of these proteins, along with ... More
Cooperative interaction between GATA-4 and GATA-6 regulates myocardial gene expression.
AuthorsCharron F, Paradis P, Bronchain O, Nemer G, Nemer M
JournalMol Cell Biol
PubMed ID10330176
Two members of the GATA family of transcription factors, GATA-4 and GATA-6, are expressed in the developing and postnatal myocardium and are equally potent transactivators of several cardiac promoters. However, several in vitro and in vivo lines of evidence suggest distinct roles for the two factors in the heart. Since ... More
Initiation of hepatitis delta virus genome replication
AuthorsDingle K, Bichko V, Zuccola H, Hogle J, Taylor J
JournalJ Virol
PubMed ID9573243
The small, 195-amino-acid form of the hepatitis delta virus (HDV) antigen (deltaAg-S) is essential for genome replication, i.e., for the transcription, processing, and accumulation of HDV RNAs. To better understand this requirement, we used purified recombinant deltaAg-S and HDV RNA synthesized in vitro to assemble high-molecular-weight ribonucleoprotein (RNP) structures. After ... More
dELL is an essential RNA polymerase II elongation factor with a general role in development.
Authors Eissenberg Joel C; Ma Jiyan; Gerber Mark A; Christensen Alan; Kennison James A; Shilatifard Ali;
JournalProc Natl Acad Sci U S A
PubMed ID12096188
Several eukaryotic proteins increase RNA polymerase II (Pol II) transcription rates in vitro. The relative contributions of these factors to gene expression in vivo is unknown. The ELL family of proteins promote Pol II elongation in vitro, and the Drosophila ELL homolog (dELL) is associated with Pol II at sites ... More
Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability.
Authors Missirlis Fanis; Ulschmid Julia K; Hirosawa-Takamori Mitsuko; Grönke Sebastian; Schäfer Ulrich; Becker Katja; Phillips John P; Jäckle Herbert;
JournalJ Biol Chem
PubMed ID11796729
Defense against oxidative stress in mammals includes the regeneration of the major thiol reductants glutathione and thioredoxin by glutathione reductase and thioredoxin reductase (TrxR), respectively. In contrast, Drosophila, and possibly insects in general, lacks glutathione reductase and must rely solely on the TrxR system. The mammalian TrxRs described so far ... More
Cloning and characterization of a calcium-binding, histamine-releasing protein from Schistosoma mansoni.
Authors Rao Kakuturu V N; Chen Lin; Gnanasekar Munirathinam; Ramaswamy Kalyanasundaram;
JournalJ Biol Chem
PubMed ID12050167
A homologue of the mammalian translationally controlled tumor protein (TCTP) was cloned from the human parasite Schistosoma mansoni (SmTCTP). Sequence analysis showed that SmTCTP differed from other reported TCTPs in having only one signature sequence. Subsequently, SmTCTP was cloned in a T7 expression system and expressed as a histidine-tagged fusion ... More
Constitutive Mutations of the OccR Regulatory Protein Affect DNA Bending in Response to Metabolites Released from Plant Tumors.
Authors Akakura Reiko; Winans Stephen C;
JournalJ Biol Chem
PubMed ID11717314
OccR is a LysR-type transcriptional regulator of Agrobacterium tumefaciens that positively regulates the octopine catabolism operon of the Ti plasmid and is also an autorepressor. Positive control of the occ genes occurs in response to octopine, a nutrient released from crown gall tumors. OccR binds to a site upstream of ... More
Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore.
Authors Measday Vivien; Hailey Dale W; Pot Isabelle; Givan Scott A; Hyland Katherine M; Cagney Gerard; Fields Stan; Davis Trisha N; Hieter Philip;
JournalGenes Dev
PubMed ID11782448
The budding yeast kinetochore is composed of an inner and outer protein complex, which binds to centromere (CEN) DNA and attaches to microtubules. We performed a genetic synthetic dosage lethality screen to identify novel kinetochore proteins in a collection of chromosome transmission fidelity mutants. Our screen identified several new kinetochore-related ... More
Cell and chloroplast division requires ARTEMIS.
Authors Fulgosi Hrvoje; Gerdes Lars; Westphal Sabine; Glockmann Christel; Soll Jurgen;
JournalProc Natl Acad Sci U S A
PubMed ID12169665
Chloroplasts are endosymbiotic organelles of cyanobacterial origin. It seems reasonable to assume that cell division and organelle division still share general principles, as shown for the FtsZ proteins. However, further components involved in this process are largely unknown. Here we describe ARTEMIS, a nuclear-encoded protein of chloroplast inner envelope membranes ... More
Hepatitis B Virus pX Interacts with HBXAP, a PHD Finger Protein to Coactivate Transcription.
Authors Shamay Meir; Barak Orr; Doitsh Gilad; Ben-Dor Israel; Shaul Yosef;
JournalJ Biol Chem
PubMed ID11788598
Hepatitis B virus (HBV) gene expression is mainly regulated at the transcription initiation level. The viral X protein (pX) is a transcription coactivator/mediator targeting TFIIB for the recruitment of RNA polymerase II. Here we report a novel pX nuclear target designated HBXAP (hepatitis B virus X-associated protein). HBXAP is a ... More