pAC5.1/V5-His vectors are designed for use in Drosophila cells to achieve high-level transient expression of recombinant proteins. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAC5.1/V5-His vectors can be used with the DES™-Inducible Kits (K512001 and K412001) for constitutive expression of your protein of interest. The pAc5.1/V5-His vectors also offer the following features:
• Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency • C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody • C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
For Research Use Only. Not for use in diagnostic procedures.
His Tag (6x), V5 Epitope Tag
Restriction Enzyme ⁄ MCS
Contents & storage
Contains 20 µg each of lyophilized pAc5.1/V5-His A, B, C and pAc5.1/V5-His/LacZ. Store at -20°C. All components are guaranteed stable for 6 months when properly stored.