Gata factor translation is the final downstream step in the amino acid/tor-mediated vitellogenin gene expression in the anautogenous mosquito aedes aegypti.
'Ingestion of blood is required for vector mosquitoes to initiate reproductive cycles determining their role as vectors of devastating human diseases. Nutritional signaling plays a pivotal role in regulating mosquito reproduction. Transcription of yolk protein precursor genes is repressed until mosquitoes take blood. Previously, we have shown that to signal ... More
Inwardly rectifying K+ (Kir) channels in Drosophila. A crucial role of cellular milieu factors Kir channel function.
'Three cDNAs encoding inwardly rectifying potassium (Kir) channels were isolated from Drosophila melanogaster. The protein sequences of Drosophila KirI (dKirI) and dKirII are moderately (<44%) and dKirIII sequence is weakly (<27%) identical to human Kir channel subunits. During fly development, five dKir channel transcripts derived from three genes are differentially ... More
Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms.
AuthorsFujita N, Takebayashi S, Okumura K, Kudo S, Chiba T, Saya H, Nakao M
JournalMol Cell Biol
PubMed ID10454587
DNA methylation of promoter-associated CpG islands is involved in the transcriptional repression of vertebrate genes. To investigate the mechanisms underlying gene inactivation by DNA methylation, we characterized a human MBD1 protein, one of the components of MeCP1, which possesses a methyl-CpG binding domain (MBD) and cysteine-rich (CXXC) domains. Four novel ... More
A baculovirus superinfection system: efficient vehicle for gene transfer into drosophila S2 cells.
AuthorsLee DF, Chen CC, Hsu TA, Juang JL
JournalJ Virol
PubMed ID11090187
The baculovirus expression vector system is considered to be a safe, powerful, but cell-lytic heterologous protein expression system in insect cells. We show here that there is a new baculovirus system for efficient gene transfer and expression using the popular and genetically well-understood Drosophila S2 cells. The recombinant baculovirus was ... More
The eukaryotic DNMT2 genes encode a new class of cytosine-5 DNA methyltransferases.
AuthorsTang LY, Reddy MN, Rasheva V, Lee TL, Lin MJ, Hung MS, Shen CK,
JournalJ Biol Chem
PubMed ID12819212
DNMT2 is a subgroup of the eukaryotic cytosine-5 DNA methyltransferase gene family. Unlike the other family members, proteins encoded by DNMT2 genes were not known before to possess DNA methyltransferase activities. Most recently, we have shown that the genome of Drosophila S2 cells stably expressing an exogenous Drosophila dDNMT2 cDNA ... More
Silencing of the mouse H-rev107 gene encoding a class II tumor suppressor by CpG methylation.
Authors Roder Karim; Latasa Maria-Jesus; Sul Hei Sook;
JournalJ Biol Chem
PubMed ID12055182
H-rev107 is a tumor suppressor originally isolated in revertants of H-ras-transformed cell lines. The gene is ubiquitously expressed in normal tissues but down-regulated in primary carcinomas or in many cell lines derived from tumors, including WEHI 7.1 lymphoma cells. Here, we show that unlike in H-rev107-expressing cells or tissues the ... More
Two isoforms of the Notch antagonist Hairless are produced by differential translation initiation.
The Notch-signaling pathway controls cellular differentiation, including proliferation and cell death in all higher metazoans (including flies and men). Signal transduction through activated Notch involves the CSL group of transcriptional regulators. Notch signals need to be tightly regulated, and in Drosophila they are antagonized by the Hairless (H) protein. H ... More