Invitrogen™

pBAD/Myc-His Kit

Catalog number: V44001
Invitrogen™

pBAD/Myc-His Kit

Catalog number: V44001
Catalog Number
V44001
also known as V440-01
Unit Size
1 kit
Price (USD)
Price: 1,114.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
V44001
also known as V440-01
1 kit
Price: 1,114.00
Your price:
-
Product Overview
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The pBAD/Myc-His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The pBAD/Myc-His vector expresses native proteins or fusion proteins with a C-terminal tag. The vector provides:

• The araBAD promoter for tightly regulated expression
• Translation initiation signals optimized for E. coliexpression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with an Anti-His(C-term) Antibody
• C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody

Three vectors are provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible System
Inducible
Inducing Agent
Arabinose
Promoter
araBAD
Product Type
Bacterial Expression Vector
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cleavage
EK (Enterokinase) Recognition Site
Protein Tag
c-Myc Epitope Tag
Cloning Method
Restriction Enzyme/MCS
Vector
pBAD

Contents & Storage

The pBAD/Myc-His Kit includes 20 μg of pBAD/Myc-His A, B, & C vector, 20 μg of pBAD/Myc-His/lacZ, 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.

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