pBAD/Myc-His Kit

Catalog number:  V44001

 Related applications:

Protein Expression

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The pBAD/Myc-His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The pBAD/Myc-His vector expresses native proteins or fusion proteins with a C-terminal tag. The vector provides:

• The araBAD promoter for tightly regulated expression
• Translation initiation signals optimized for E. coliexpression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with an Anti-His(C-term) Antibody
• C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody

Three vectors are provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
For Research Use Only. Not for use in diagnostic procedures.



Vector Type: pBAD
Cloning Method: Restriction Enzyme ⁄ MCS
Promoter: araBAD
Protein Tag or Fusion: c-Myc Epitope Tag
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Inducing Agent: Arabinose
Product Size: 1 kit
Selection Agent (Eukaryotic): None

Contents & storage

The pBAD/Myc-His Kit includes 20 µg of pBAD/Myc-His A, B, & C vector, 20 µg of pBAD/Myc-His/lacZ, 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.


Manuals & protocols