The intercellular signaling activity of the Mycobacterium tuberculosis chaperonin 60.1 protein resides in the equatorial domain.
AuthorsTormay P, Coates AR, Henderson B,
JournalJ Biol Chem
PubMed ID15677470
'The major heat shock protein, chaperonin 60, has been established to have intercellular signaling activity in addition to its established protein-folding function. Mycobacterium tuberculosis is one of a small proportion of bacteria to encode two chaperonin 60 proteins. We have demonstrated that chaperonin 60.1 from this bacterium is a very ... More
Novel pathway for arsenic detoxification in the legume symbiont Sinorhizobium meliloti.
'We report a novel pathway for arsenic detoxification in the legume symbiont Sinorhizobium meliloti. Although a majority of ars operons consist of three genes, arsR (transcriptional regulator), arsB [As(OH)3/H+ antiporter], and arsC (arsenate reductase), the S. meliloti ars operon includes an aquaglyceroporin (aqpS) in place of arsB. The presence of ... More
CopA: An Escherichia coli Cu(I)-translocating P-type ATPase
AuthorsRensing C, Fan B, Sharma R, Mitra B, Rosen BP
JournalProc Natl Acad Sci U S A
PubMed ID10639134
'The copA gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in Escherichia coli. The copA gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other ... More
The prodrug activator EtaA from Mycobacterium tuberculosis is a Baeyer-Villiger monooxygenase.
'EtaA is a newly identified FAD-containing monooxygenase that is responsible for activation of several thioamide prodrugs in Mycobacterium tuberculosis. It was found that purified EtaA displays a remarkably low activity with the antitubercular prodrug ethionamide. Hinted by the presence of a Baeyer-Villiger monooxygenase sequence motif in the EtaA sequence, we ... More
Genetic engineering of phytochrome biosynthesis in bacteria.
Authors Gambetta G A; Lagarias J C;
JournalProc Natl Acad Sci U S A
PubMed ID11553807
The bilin prosthetic groups of the phytochrome photoreceptors and the light-harvesting phycobiliprotein antennae arise from the oxygen-dependent ring opening of heme. Two ferredoxin-dependent enzymes contribute to this conversion: a heme oxygenase and a bilin reductase with discrete double-bond specificity. Using a dual plasmid system, one expressing a truncated cyanobacterial apophytochrome ... More
Identification of promoter elements in mycobacteria: mutational analysis of a highly symmetricdual promoter directing the expression of replication genes of the Mycobacterium plasmidpAL5000.
AuthorsStolt P, Zhang Q, Ehlers S
JournalNucleic Acids Res
PubMed ID9862957
The 120 bp origin of replication (ori) for the Mycobacterium plasmid pAL5000 has been shown to comprise the binding sites for the replication protein RepB as well as the start site of transcription for the repA and repB genes, encoding the replication proteins RepA and RepB. In this work it ... More