New Protein Expression Support Center—find Mammalian Expression technical tips and troubleshooting recommendations from our scientists.
Invitrogen™

pcDNA™3.1 (+) Mammalian Expression Vector

Catalog number:  V79020

Related applications:

Protein Expression

Back to top

Description

This pcDNA™3.1(+) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.

The pcDNA™3.1 Expression Vector Family
Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin®, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:

• Cytomegalovirus (CMV) enhancer-promoter for high-level expression
• Large multiple cloning site in either forward (+) or reverse (-) orientations
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7)
• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cloning Method: Restriction Enzyme ⁄ MCS
Key Functions: Constitutive Expression
Product Line: pcDNA™
Delivery Type: Transfection
Promoter: CMV
Selection Agent (Eukaryotic): Geneticin® (G-418)
Vector: pcDNA
Protein Tag: Untagged

Contents & storage

20 µg of this pcDNA™3.1(+) vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.