Two murine homologs of the Drosophila single-minded protein that interact with the mouse aryl hydrocarbon receptor nuclear translocator protein.
AuthorsProbst MR,Fan CM,Tessier-Lavigne M,Hankinson O
JournalThe Journal of biological chemistry
PubMed ID9020169
Evaluation of VP22 spread in tissue culture.
AuthorsBrewis N,Phelan A,Webb J,Drew J,Elliott G,O'Hare P
JournalJournal of virology
PubMed ID10623773
We compare methods of detection of intercellular transport of the herpes simplex virus protein VP22 and of a green fluorescent protein (GFP)-VP22 fusion protein. Spread of both proteins was observed by immunofluorescence (IF) using organic fixatives. Spread of both proteins was also detected by IF after paraformaldehyde (PFA) fixation and ... More
A kinase-regulated PDZ-domain interaction controls endocytic sorting of the beta2-adrenergic receptor.
AuthorsCao TT,Deacon HW,Reczek D,Bretscher A,von Zastrow M
JournalNature
PubMed ID10499588
Molecular cloning and biological activity of a novel lysyl oxidase-related gene expressed in cartilage.
We cloned a cDNA encoding a novel lysyl oxidase-related protein, named LOXC, by suppression subtractive hybridization between differentiated and calcified ATDC5 cells, a clonal mouse chondrogenic EC cell line. The deduced amino acid sequence of mouse LOXC consists of 757 amino acids and shows 50% identity with that of mouse ... More
The large size (six membrane-spanning repeats in each of four domains) and asymmetric architecture of the voltage-dependent Na+ channel has hindered determination of its structure. With the goal of determining the minimum structure of the Na+ channel permeation pathway, we created two stable cell lines expressing the voltage-dependent rat skeletal ... More
Comm sorts robo to control axon guidance at the Drosophila midline.
'Axon growth across the Drosophila midline requires Comm to downregulate Robo, the receptor for the midline repellent Slit. We show here that comm is required in neurons, not in midline cells as previously thought, and that it is expressed specifically and transiently in commissural neurons. Comm acts as a sorting ... More
Association of Bcr-Abl with the Proto-oncogene Vav Is Implicated in Activation of the Rac-1 Pathway.
Authors Bassermann Florian; Jahn Thomas; Miething Cornelius; Seipel Petra; Bai Ren-Yuan; Coutinho Sunita; Tybulewicz Victor L; Peschel Christian; Duyster Justus;
JournalJ Biol Chem
PubMed ID11790798
'Vav is a guanine nucleotide exchange factor for the Rho/Rac family predominantly expressed in hematopoietic cells and implicated in cell proliferation and cytoskeletal organization. The oncogenic tyrosine kinase Bcr-Abl has been shown to activate Rac-1, which is important for Bcr-Abl induced leukemogenesis. Previous studies by Matsuguchi et al. (Matsuguchi, T., ... More
Transcriptionally active polymerase chain reaction (TAP): high throughput gene expression using genome sequence data.
Authors Liang Xiaowu; Teng Andy; Braun Dawn M; Felgner Jiin; Wang Yan; Baker Scott I; Chen Shizong; Zelphati Olivier; Felgner Philip L;
JournalJ Biol Chem
PubMed ID11713261
'An approach is described for making transcriptionally active PCR (TAP) fragments that were used directly in in vitro and in vivo expression experiments. TAP fragments encoding reporter genes were amplified in 1 day using typical PCR methodology and were expressed in cultured cells and in mice at levels comparable with ... More
Utilization of MHC class I transgenic mice for development of minigene DNA vaccinesencoding multiple HLA-restricted CTL epitopes.
AuthorsIshioka GY, Fikes J, Hermanson G, Livingston B, Crimi C, Qin M, del Guercio MF, Oseroff C, Dahlberg C, Alexander J, Chesnut RW, Sette A
JournalJ Immunol
PubMed ID10201910
'We engineered a multiepitope DNA minigene encoding nine dominant HLA-A2.1- and A11-restricted epitopes from the polymerase, envelope, and core proteins of hepatitis B virus and HIV, together with the PADRE (pan-DR epitope) universal Th cell epitope and an endoplasmic reticulum-translocating signal sequence. Immunization of HLA transgenic mice with this construct ... More
Covalent attachment of the SUMO-1 protein to the negative regulatory domain of the c-Myb transcription factor modifies its stability and transactivation capacity.
Authors Bies Juraj; Markus Ján; Wolff Linda;
JournalJ Biol Chem
PubMed ID11779867
'The transcription factor c-Myb is subject to several types of post-translational modifications, including phosphorylation, acetylation, and ubiquitination. These modifications regulate the transcription and transforming activity as well as the proteolytic stability of c-Myb. Here we report the covalent modification of c-Myb with the small ubiquitin-related protein SUMO-1. Mutational analysis identified ... More
The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of delta-catenin and ARVCF.
Authors Laura Richard P; Witt Andrea S; Held Heike A; Gerstner Resi; Deshayes Kurt; Koehler Michael F T; Kosik Kenneth S; Sidhu Sachdev S; Lasky Laurence A;
JournalJ Biol Chem
PubMed ID11821434
'Erbin is a recently described member of the LAP (leucine-rich repeat and PDZ domain) protein family. We used a C-terminally displayed phage peptide library to identify optimal ligands for the Erbin PDZ domain. Phage-selected peptides were type 1 PDZ ligands that bound with high affinity and specificity to the Erbin ... More
Nef interacts with the mu subunit of clathrin adaptor complexes and reveals a cryptic sorting signal in MHC I molecules.
AuthorsLe Gall S, Erdtmann L, Benichou S, Berlioz-Torrent C, Liu L, Benarous R, Heard JM, Schwartz O
JournalImmunity
PubMed ID9586638
'The surface expression of MHC I is reduced in HIV-infected cells. We show that the Nef protein affects the intracellular sorting of HLA-A and -B molecules. In the presence of Nef, these proteins accumulate in the Golgi and colocalize with clathrin-coated vesicles. MHC I modulation relies on a tyrosine-based sorting ... More
Potentiation of serum response factor activity by a family of myocardin-related transcription factors.
Authors Wang Da-Zhi; Li Shijie; Hockemeyer Dirk; Sutherland Lillian; Wang Zhigao; Schratt Gerhard; Richardson James A; Nordheim Alfred; Olson Eric N;
JournalProc Natl Acad Sci U S A
PubMed ID12397177
'Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac ... More
The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM.
AuthorsSayos J, Wu C, Morra M, Wang N, Zhang X, Allen D, van Schaik S, Notarangelo L, Geha R, Roncarolo MG, Oettgen H, De Vries JE, Aversa G, Terhorst C
JournalNature
PubMed ID9774102
'In addition to triggering the activation of B- or T-cell antigen receptors, the binding of a ligand to its receptor at the cell surface can sometimes determine the physiological outcome of interactions between antigen-presenting cells, T and B lymphocytes. The protein SLAM (also known as CDw150), which is present on ... More
p110delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes.
AuthorsChantry D, Vojtek A, Kashishian A, Holtzman DA, Wood C, Gray PW, Cooper JA, Hoekstra MF
JournalJ Biol Chem
PubMed ID9235916
'We have identified a novel p110 isoform of phosphatidylinositol 3- kinase from human leukocytes that we have termed p110delta. In addition, we have independently isolated p110delta from a mouse embryo library on the basis of its ability to interact with Ha-RasV12 in the yeast two-hybrid system. This unique isoform contains ... More
Distinct STAT structure promotes interaction of STAT2 with the p48 subunit of the interferon-alpha-stimulated transcription factor ISGF3.
AuthorsMartinez-Moczygemba M, Gutch MJ, French DL, Reich NC
JournalJ Biol Chem
PubMed ID9242679
'Cells express a variety of STAT (signal transducer and activator of transcription) transcription factors that are structurally homologous and yet function specifically in response to particular cytokines. The functions of the individual STATs are dependent on distinct protein- protein interactions. STAT1 and STAT2 are activated by tyrosine phosphorylation in response ... More
NF-Y is associated with the histone acetyltransferases GCN5 and P/CAF.
AuthorsCurrie RA
JournalJ Biol Chem
PubMed ID9430679
'The ubiquitous transcription factor, NF-Y, plays a pivotal role in the cell cycle regulation of the mammalian cyclin A, cdc25C, and cdc2 genes, in the S-phase activation of the ribonucleotide reductase R2 gene, in addition to its critical role as a key proximal promoter factor in the transcriptional regulation of ... More
Presenilins are processed by caspase-type proteases.
AuthorsLoetscher H, Deuschle U, Brockhaus M, Reinhardt D, Nelboeck P, Mous J, Grunberg J, Haass C, Jacobsen H
JournalJ Biol Chem
PubMed ID9252383
'Presenilin 1 (PS1) and presenilin 2 (PS2) are endoproteolytically processed in vivo and in cell transfectants to yield 27-35-kDa N- terminal and 15-24-kDa C-terminal fragments. We have studied the cleavage of PS1 and PS2 in transiently and stably transfected hamster kidney and mouse and human neuroblastoma cells by immunoblot and ... More
A novel N-terminal splice variant of the rat H+-K+-ATPase alpha2 subunit. Cloning, functional expression, and renal adaptive response to chronic hypokalemia.
AuthorsKone BC, Higham SC
JournalJ Biol Chem
PubMed ID9446555
'The H+-K+-ATPase of renal collecting duct mediates K+ conservation during chronic hypokalemia. K+ deprivation promotes H+-K+-ATPase alpha2 (HKalpha2) gene expression in the medullary collecting duct, the principal site of active K+ reabsorption, suggesting that this isozyme contributes to renal K+ reclamation. We report here that alternative transcriptional initiation and mRNA ... More
Characterization of an antigen that is recognized on a melanoma showing partial HLA loss by CTL expressing an NK inhibitory receptor.
AuthorsIkeda H, Lethe B, Lehmann F, van Baren N, Baurain JF, de Smet C, Chambost H, Vitale M, Moretta A, Boon T, Coulie PG
JournalImmunity
PubMed ID9047241
'Melanoma lines MEL.A and MEL.B were derived from metastases removed from patient LB33 in 1988 and 1993, respectively. The MEL.A cells express several antigens recognized by autologous cytolytic T lymphocytes (CTL) on HLA class I molecules. The MEL.B cells have lost expression of all class I molecules except for HLA-A24. ... More
A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing.
AuthorsCella M, Dohring C, Samaridis J, Dessing M, Brockhaus M, Lanzavecchia A, Colonna M
JournalJ Exp Med
PubMed ID9151699
'Immunoglobulin-like transcript (ILT) 3 is a novel cell surface molecule of the immunoglobulin superfamily, which is selectively expressed by myeloid antigen presenting cells (APCs) such as monocytes, macrophages, and dendritic cells. The cytoplasmic region of ILT3 contains putative immunoreceptor tyrosine-based inhibitory motifs that suggest an inhibitory function of ILT3. Indeed, ... More
Metaxin is a component of a preprotein import complex in the outer membrane of the mammalian mitochondrion.
AuthorsArmstrong LC, Komiya T, Bergman BE, Mihara K, Bornstein P
JournalJ Biol Chem
PubMed ID9045676
'Metaxin, a novel gene located between the glucocerebrosidase and thrombospondin 3 genes in the mouse, is essential for survival of the postimplantation mouse embryo. In this study, the subcellular location, domain structure, and biochemical function of metaxin were investigated. Anti-recombinant metaxin antibodies recognized 35- and 70- kDa proteins in mitochondria ... More
Core protein dependence of epimerization of glucuronosyl residues in galactosaminoglycans.
Authors Seidler Daniela G; Breuer Egon; Grande-Allen K Jane; Hascall Vincent C; Kresse Hans;
JournalJ Biol Chem
PubMed ID12207034
'Chondroitin sulfate and dermatan sulfate proteoglycans are distinguished by differences in their proportion of d-glucuronosyl and l-iduronosyl residues, the latter being formed by chondroitin-glucuronate 5-epimerase during or after glycosaminoglycan chain polymerization. To investigate the influence of the core protein on the extent of epimerization, we expressed chimeric proteins in 293 ... More
TATA-binding protein-free TAF-containing complex (TFTC) and p300 are both required for efficient transcriptional activation.
AuthorsHardy S, Brand M, Mittler G, Yanagisawa J, Kato S, Meisterernst M, Tora L.
JournalJ Biol Chem
PubMed ID12107188
'Initiation of transcription of protein-encoding genes by RNA polymerase II was thought to require transcription factor TFIID, a complex comprising the TATA-binding protein (TBP) and TBP-associated factors (TAFs). In the presence of TBP-free TAF complex (TFTC), initiation of polymerase II transcription can occur in the absence of TFIID. TFTC contains ... More
A Principal Role for the Proteasome in Endoplasmic Reticulum-associated Degradation of Misfolded Intracellular Cystic Fibrosis Transmembrane Conductance Regulator.
Authors Gelman Marina S; Kannegaard Elisa S; Kopito Ron R;
JournalJ Biol Chem
PubMed ID11812794
'Endoplasmic reticulum-associated degradation of misfolded cystic fibrosis transmembrane conductance regulator (CFTR) protein is known to involve the ubiquitin-proteasome system. In addition, an ATP-independent proteolytic system has been suggested to operate in parallel with this pathway and become up-regulated when proteasomes are inhibited (Jensen, T. J., Loo, M. A., Pind, S., ... More
Smac agonists sensitize for Apo2L/TRAIL- or anticancer drug-induced apoptosis and induce regression of malignant glioma in vivo.
'A major concern in cancer therapy is resistance of tumors such as glioblastoma to current treatment protocols. Here, we report that transfer of the gene encoding second mitochondria-derived activator of caspase (Smac) or Smac peptides sensitized various tumor cells in vitro and malignant glioma cells in vivo for apoptosis induced ... More
SMAC Negatively Regulates the Anti-apoptotic Activity of Melanoma Inhibitor of Apoptosis (ML-IAP).
Authors Vucic Domagoj; Deshayes Kurt; Ackerly Heidi; Pisabarro Maria Teresa; Kadkhodayan Saloumeh; Fairbrother Wayne J; Dixit Vishva M;
JournalJ Biol Chem
PubMed ID11801603
'Inhibitors of apoptosis (IAPs) physically interact with a variety of pro-apoptotic proteins and inhibit apoptosis induced by diverse stimuli. X-linked IAP (X-IAP) is a prototype IAP family member that inhibits several caspases, the effector proteases of apoptosis. The inhibitory activity of X-IAP is regulated by SMAC, a protein that is ... More
Repression of Na,K-ATPase beta1-subunit by the transcription factor snail in carcinoma.
AuthorsEspineda CE, Chang JH, Twiss J, Rajasekaran SA, Rajasekaran AK,
JournalMol Biol Cell
PubMed ID14699059
'The Na,K-ATPase consists of two essential alpha- and beta-subunits and regulates the intracellular Na+ and K+ homeostasis. Although the alpha-subunit contains the catalytic activity, it is not active without functional beta-subunit. Here, we report that poorly differentiated carcinoma cell lines derived from colon, breast, kidney, and pancreas show reduced expression ... More
Sphingosine 1-Phosphate, Present in Serum-derived Lipoproteins, Activates Matriptase.
Authors Benaud Christelle; Oberst Michael; Hobson John P; Spiegel Sarah; Dickson Robert B; Lin Chen-Yong;
JournalJ Biol Chem
PubMed ID11792696
'We describe here a novel biological function of sphingosine 1-phosphate (S1P): the activation of a serine protease, matriptase. Matriptase is a type II integral membrane serine protease, expressed on the surface of a variety of epithelial cells; it may play an important role in tissue remodeling. We have previously reported ... More
Interaction codes within the family of mammalian Phox and Bem1p domain-containing proteins.
AuthorsLamark T, Perander M, Outzen H, Kristiansen K, Øvervatn A, Michaelsen E, Bjørkøy G, Johansen T,
JournalJ Biol Chem
PubMed ID12813044
'The Phox and Bem1p (PB1) domain constitutes a recently recognized protein-protein interaction domain found in the atypical protein kinase C (aPKC) isoenzymes, lambda/iota- and zeta PKC; members of mitogen-activated protein kinase (MAPK) modules like MEK5, MEKK2, and MEKK3; and in several scaffold proteins involved in cellular signaling. Among the last ... More
JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes.
Authors Ostermann Georg; Weber Kim S C; Zernecke Alma; Schröder Andreas; Weber Christian;
JournalNat Immunol
PubMed ID11812986
'Inflammatory recruitment of leukocytes is governed by dynamic interactions between integrins and endothelial immunoglobulin superfamily (IgSF) proteins. We have identified the IgSF member junctional adhesion molecule 1 (JAM-1) as a ligand of the beta(2) integrin lymphocyte function-associated antigen 1 (LFA-1). Under static and physiological flow conditions, JAM-1 contributed to LFA-1-dependent ... More
Hyporesponsiveness to vaccination with Borrelia burgdorferi OspA in humans and in TLR1- and TLR2-deficient mice.
Authors Alexopoulou Lena; Thomas Venetta; Schnare Markus; Lobet Yves; Anguita Juan; Schoen Robert T; Medzhitov Ruslan; Fikrig Erol; Flavell Richard A;
JournalNat Med
PubMed ID12091878
'The Lyme disease vaccine is based on the outer-surface lipoprotein (OspA) of the pathogen Borrelia burgdorferi, and 95% of vaccine recipients develop substantial titers of antibodies against OspA. Here, we identified seven individuals with very low antibody titers after vaccination (low responders). The macrophages of low responders produced less tumor ... More
Upstream stimulatory factors binding to an E box motif in the R region of the bovine leukemia virus long terminal repeat stimulates viral gene expression.
Authors Calomme Claire; Nguyen Thi Lien-Anh; de Launoit Yvan; Kiermer Veronique; Droogmans Louis; Burny Arsene; Van Lint Carine;
JournalJ Biol Chem
PubMed ID11741930
'The bovine leukemia virus (BLV) promoter is located in its 5''-long terminal repeat and is composed of the U3, R, and U5 regions. BLV transcription is regulated by cis-acting elements located in the U3 region, including three 21-bp enhancers required for transactivation of the BLV promoter by the virus-encoded transactivator ... More
A minimally lipidated form of cell-derived apolipoprotein E exhibits isoform-specific stimulation of neurite outgrowth in the absence of exogenous lipids or lipoproteins.
AuthorsDeMattos RB, Curtiss LK, Williams DL
JournalJ Biol Chem
PubMed ID9461617
'Within the central nervous system, apolipoprotein E (apoE) synthesis is increased in response to nerve injury, a finding that may reflect a role for apoE in neuronal remodeling. Recent studies show that apoE3 promotes and apoE4 inhibits neurite outgrowth in cultured neuronal cells. Interestingly, these isoform-specific effects are observed only ... More
Agonist regulation of human beta2-adrenergic receptor mRNA stability occurs via a specific AU-rich element.
AuthorsDanner S, Frank M, Lohse MJ
JournalJ Biol Chem
PubMed ID9452435
'Prolonged agonist stimulation of beta2-adrenergic receptors results in receptor down-regulation, which is closely associated with a reduction of the corresponding mRNA, an effect mediated in part by changes in mRNA stability. Transfection experiments with human beta2-adrenergic receptor cDNAs bearing or lacking the untranslated regions suggested that the essential agonist sensitivity ... More
Requirement of Src kinase Lyn for induction of DNA synthesis by granulocyte colony-stimulating factor.
AuthorsCorey SJ, Dombrosky-Ferlan PM, Zuo S, Krohn E, Donnenberg AD, Zorich P, Romero G, Takata M, Kurosaki T
JournalJ Biol Chem
PubMed ID9452436
'Treatment of cells with granulocyte colony-stimulating factor (G-CSF) leads to tyrosine phosphorylation of cellular proteins. G-CSF stimulates both the activation of protein tyrosine kinases Lyn, Jak1, and Jak2 and the association of these enzymes with the G-CSF receptor. Wild-type, lyn-deficient, and syk-deficient chicken B lymphocyte cell lines were transfected with ... More
The nuclear LIM domain interactor NLI mediates homo- and heterodimerization of LIM domain transcription factors.
AuthorsJurata LW, Pfaff SL, Gill GN
JournalJ Biol Chem
PubMed ID9452425
'LIM domain-containing transcription factors are required for embryonic survival and for the determination of many cell types. The combinatorial expression of the LIM homeodomain proteins Isl1, Isl2, Lhx1, and Lhx3 in subsets of developing motor neurons correlates with the future organization of these neurons into motor columns with distinct innervation ... More
Visualization of agonist-induced sequestration and down-regulation of a green fluorescent protein-tagged beta2-adrenergic receptor.
AuthorsKallal L, Gagnon AW, Penn RB, Benovic JL
JournalJ Biol Chem
PubMed ID9417083
'To date, the visualization of beta2-adrenergic receptor (beta2AR) trafficking has been largely limited to immunocytochemical analyses of acute internalization events of epitope-tagged receptors in various transfection systems. The development of a beta2AR conjugated with green fluorescent protein (beta2AR-GFP) provides the opportunity for a more extensive optical analysis of beta2AR sequestration, ... More
Ligand binding to macrophage scavenger receptor-A induces urokinase- type plasminogen activator expression by a protein kinase-dependent signaling pathway.
AuthorsHsu HY, Hajjar DP, Khan KM, Falcone DJ
JournalJ Biol Chem
PubMed ID9422792
'Macrophage scavenger receptor-type A (MSR-A) has been implicated in the transmission of cell signals and the regulation of diverse cellular functions (Falcone, D. J., and Ferenc, M. J. (1988) J. Cell. Physiol. 135, 387-396; Falcone, D. J., McCaffrey, T. A., and Vergilio, J. A. (1991) J. Biol. Chem. 266, 22726-22732; ... More
Stable expression of the human kinin B1 receptor in Chinese hamster ovary cells. Characterization of ligand binding and effector pathways.
AuthorsAustin CE, Faussner A, Robinson HE, Chakravarty S, Kyle DJ, Bathon JM, Proud D
JournalJ Biol Chem
PubMed ID9111052
'To delineate ligand binding and functional characteristics of the human B1 kinin receptor, a stable clone of Chinese hamster ovary cells expressing a single class of binding sites for [3H]des-Arg10- lysylbradykinin with a Kd of 0.3 nM and a Bmax of 38 fmol/mg protein ( approximately 40,000 receptors/cell) was isolated. ... More
Multiple promoter elements differentially regulate the expression of the mouse tenascin gene.
AuthorsCopertino DW, Edelman GM, Jones FS
JournalProc Natl Acad Sci U S A
PubMed ID9050867
'Tenascin (TN) is an extracellular matrix glycoprotein that is expressed in a characteristic spatiotemporal pattern during development and is up- regulated in the adult during tumorigenesis, wound healing, and nerve regeneration. In previous studies, we identified a promoter within the proximal 250 bp upstream of the mouse TN gene that ... More
c-Myb trans-activates the human DNA topoisomerase IIalpha gene promoter.
'DNA topoisomerase IIalpha (topo IIalpha) is an essential proliferation- dependent nuclear enzyme which has been exploited as an anti-tumor drug target. Since the proliferative status of human leukemia cells is associated with expression of the c-myb proto-oncogene, c-Myb was investigated as a trans-activator of the topo IIalpha gene. Using topo ... More
A functional interaction between the carboxy-terminal domain of RNA polymerase II and pre-mRNA splicing.
AuthorsDu L, Warren SL
JournalJ Cell Biol
PubMed ID9008699
'In the preceding study we found that Sm snRNPs and SerArg (SR) family proteins co-immunoprecipitate with Pol II molecules containing a hyperphosphorylated CTD (Kim et al., 1997). The association between Pol IIo and splicing factors is maintained in the absence of pre-mRNA, and the polymerase need not be transcriptionally engaged ... More
Intestinal trefoil factor confers colonic epithelial resistance to apoptosis
AuthorsTaupin DR, Kinoshita K, Podolsky DK
JournalProc Natl Acad Sci U S A
PubMed ID10639160
'Intestinal trefoil factor (ITF) is an essential regulator of colonic epithelial restitution, the rapid migration of colonocytes over mucosal wounds. High levels of ITF are frequently present in colorectal cancers and derived cell lines. Mucosal restitution requires the detachment of epithelium from substrate, which would be expected to induce apoptosis. ... More
Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10.
AuthorsIdo H, Harada K, Futaki S, Hayashi Y, Nishiuchi R, Natsuka Y, Li S, Wada Y, Combs AC, Ervasti JM, Sekiguchi K,
JournalJ Biol Chem
PubMed ID14701821
'The adhesive interactions of cells with laminins are mediated by integrins and non-integrin-type receptors such as alpha-dystroglycan and syndecans. Laminins bind to these receptors at the C-terminal globular domain of their alpha chains, but the regions recognized by these receptors have not been mapped precisely. In this study, we sought ... More
Cloning and functional expression of a mammalian gene for a peroxisomal sarcosine oxidase.
AuthorsReuber BE, Karl C, Reimann SA, Mihalik SJ, Dodt G
JournalJ Biol Chem
PubMed ID9045710
'Sarcosine oxidation in mammals occurs via a mitochondrial dehydrogenase closely linked to the electron transport chain. An additional H2O2- producing sarcosine oxidase has now been purified from rabbit kidney. A corresponding cDNA was cloned from rabbit liver and the gene designated sox. This rabbit sox gene encodes a protein of ... More
Recombinational construction in Escherichia coli of infectious adenoviral genomes.
AuthorsCrouzet J, Naudin L, Orsini C, Vigne E, Ferrero L, Le Roux A, Benoit P, Latta M, Torrent C, Branellec D, Denefle P, Mayaux JF, Perricaudet M, Yeh P
JournalProc Natl Acad Sci U S A
PubMed ID9037067
'A two-step gene replacement procedure was developed that generates infectious adenoviral genomes through homologous recombination in Escherichia coli. As a prerequisite, a human adenovirus serotype 5 (Ad5)-derived genome was first introduced as a PacI restriction fragment into an incP-derived replicon which, in contrast to ColE1- derivatives (e.g., pBR322 or pUC ... More
Chimeric erythropoietin-interferon gamma receptors reveal differences in functional architecture of intracellular domains for signal transduction.
AuthorsMuthukumaran G, Kotenko S, Donnelly R, Ihle JN, Pestka S
JournalJ Biol Chem
PubMed ID9030561
'Binding of interferon gamma (IFN-gamma) causes oligomerization of the two interferon gamma receptor (IFN-gammaR) subunits, receptor chain 1 (IFN-gammaR1, the ligand-binding chain) and the second chain of the receptor (IFN-gammaR2), and causes activation of two Jak kinases (Jak1 and Jak2). In contrast, the erythropoietin receptor (EpoR) requires only one receptor ... More
The human A33 antigen is a transmembrane glycoprotein and a novel member of the immunoglobulin superfamily.
AuthorsHeath JK, White SJ, Johnstone CN, Catimel B, Simpson RJ, Moritz RL, Tu GF, Ji H, Whitehead RH, Groenen LC, Scott AM, Ritter G, Cohen L, Welt S, Old LJ, Nice EC, Burgess AW
JournalProc Natl Acad Sci U S A
PubMed ID9012807
'The mAb A33 detects a membrane antigen that is expressed in normal human colonic and small bowel epithelium and > 95% of human colon cancers. It is absent from most other human tissues and tumor types. The murine A33 mAb has been shown to target colon cancer in clinical trials, ... More
Intercellular calcium signaling via gap junction in connexin-43- transfected cells [published erratum appears in J Biol Chem 1998 Aug 28;273(35):22856]
AuthorsToyofuku T, Yabuki M, Otsu K, Kuzuya T, Hori M, Tada M
JournalJ Biol Chem
PubMed ID9430691
'In excitable cells, intracellular Ca2+ is released via the ryanodine receptor from the intracellular Ca2+ storing structure, the sarcoplasmic reticulum. To determine whether this released Ca2+ propagates through gap junctions to neighboring cells and thereby constitutes a long range signaling network, we developed a cell system in which cells expressing ... More
Transcriptional repression by binding of poly(ADP-ribose) polymerase to promoter sequences.
Authors Soldatenkov Viatcheslav A; Chasovskikh Sergey; Potaman Vladimir N; Trofimova Irina; Smulson Mark E; Dritschilo Anatoly;
JournalJ Biol Chem
PubMed ID11684688
'Poly(ADP-ribose) polymerase (PARP) is a DNA-binding enzyme that plays roles in response to DNA damage, apoptosis, and genetic stability. Recent evidence has implicated PARP in transcription of eukaryotic genes. However, the existing paradigm tying PARP function to the presence of DNA strand breaks does not provide a mechanism by which ... More
Mutation analysis of the Pip interaction domain reveals critical residues for protein-protein interactions.
AuthorsOrtiz MA, Light J, Maki RA, Assa-Munt N
JournalProc Natl Acad Sci U S A
PubMed ID10077581
'The PU.1 interaction partner (Pip) is a member of the interferon regulatory factor family that regulates gene expression through heterodimerization with the ETS transcription factor PU.1. Binding of Pip alone to DNA is weak, and usually it is recruited by phosphorylated PU.1 to form a strong ternary complex with specific ... More
Immunoadjuvant activities of E. coli- and plasmid-expressed recombinant chicken IFN-alpha/beta, IFN-gamma and IL-1beta in 1-day- and 3-week-old chickens.
AuthorsSchijns VE, Weining KC, Nuijten P, Rijke EO, Staeheli P
JournalVaccine
PubMed ID10715530
'In the present study we assessed the capacity of recombinant E. coli- or plasmid-expressed chicken interferons (IFN) and chicken IL-1beta, to exert immunostimulatory activities for humoral immune responses, in day-old and adult chickens. We observed that both recombinant E. coli-expressed chicken IFN-alpha/beta and IFN-gamma facilitated the induction of a primary ... More
Plasminogen Activator Inhibitor-1 and -3 Increase Cell Adhesion and Motility of MDA-MB-435 Breast Cancer Cells.
Authors Palmieri Diane; Lee Jung Weon; Juliano Rudy L.; Church Frank C.;
JournalJ Biol Chem
PubMed ID12176977
'Plasminogen activator inhibitor-1 (PAI-1), an inhibitor of urokinase plasminogen activator, is paradoxically associated with a poor prognosis in breast cancer. PAI-1 is linked to several processes in the metastatic cascade. However, the role of PAI-1 in metastatic processes, which may be independent of protease inhibitory activity, is not fully understood. ... More
GLI activates Transcription through a Herpes Simplex Viral Protein 16 like Activation Domain
AuthorsJoon Won Yoon, Cheng Zheng Liu, Jain Tao, Yang, Rachel Swart, Philip Iannaccone, and David Walterhouse
JournalJ Biol Chem
PubMed ID9452474
'Three proteins have been identified in mammals, GLI, GLI2, and GLI3, which share a highly conserved zinc finger domain with Drosophila Cubitus interruptus and are believed to function as transcription factors in the vertebrate Sonic hedgehog-Patched signaling pathway. To understand the role GLI plays in the Sonic hedgehog-Patched pathway and ... More
Alternative Splicing of the Adenylyl Cyclase Stimulatory G-protein Galpha s Is Regulated by SF2/ASF and Heterogeneous Nuclear Ribonucleoprotein A1 (hnRNPA1) and Involves the Use of an Unusual TG 3'-Splice Site.
Authors Pollard Alison J; Krainer Adrian R; Robson Stephen C; Europe-Finner G Nicholas;
JournalJ Biol Chem
PubMed ID11825891
'The factors involved in regulating alternative splicing of the human adenylyl cyclase stimulatory G-protein Galpha(s) in different cell types remain undefined. We have designed a Galpha(s) minigene that retains the signals required for Galpha(s) alternative splicing in vivo. Employing transient transfection of human myometrial smooth muscle cells and HeLa cells, ... More
Ligand binding of the second PDZ domain regulates clustering of PSD-95 with the Kv1.4 potassium channel.
Authors Imamura Fumiaki; Maeda Shoji; Doi Tomoko; Fujiyoshi Yoshinori;
JournalJ Biol Chem
PubMed ID11723117
'The molecular mechanisms underlying the protein assembly at synaptic junctions are thought to be important for neural functions. PSD-95, one of the major postsynaptic density proteins, is composed of three PDZ domains (PDZ1, PDZ2, and PDZ3), an SH3 domain, and a GK (guanylate kinase ) domain. It binds to the ... More
Adenoviral expression of vascular endothelial growth factor-C induces lymphangiogenesis in the skin.
Authors Enholm B; Karpanen T; Jeltsch M; Kubo H; Stenback F; Prevo R; Jackson D G; Yla-Herttuala S; Alitalo K;
JournalCirc Res
PubMed ID11282897
'The growth of blood and lymphatic vasculature is mediated in part by secreted polypeptides of the vascular endothelial growth factor (VEGF) family. The prototype VEGF binds VEGF receptor (VEGFR)-1 and VEGFR-2 and is angiogenic, whereas VEGF-C, which binds to VEGFR-2 and VEGFR-3, is either angiogenic or lymphangiogenic in different assays. ... More
The interleukin-1 receptor accessory protein (IL-1RAcP) is essential for IL-1-induced activation of interleukin-1 receptor-associated kinase (IRAK) and stress-activated protein kinases (SAP kinases).
AuthorsWesche H, Korherr C, Kracht M, Falk W, Resch K, Martin MU
JournalJ Biol Chem
PubMed ID9065432
'Interleukin-1 (IL-1) is a central mediator of the immune system involved in acute and chronic inflammatory responses. Although the sequences of two types of IL-1 receptors are known, the exact molecular events resulting in signal transduction and coupling to downstream signaling elements remain unclear. The recently cloned IL-1 receptor accessory ... More
Regulation of the low molecular weight phosphotyrosine phosphatase by phosphorylation at tyrosines 131 and 132.
AuthorsTailor P, Gilman J, Williams S, Couture C, Mustelin T
JournalJ Biol Chem
PubMed ID9038134
'Activation of resting T lymphocytes is initiated by rapid but transient tyrosine phosphorylation of a number of cellular proteins. Several protein tyrosine kinases and protein tyrosine phosphatases are known to be important for this response. Here we report that normal T lymphocytes express the B isoform of low molecular weight ... More
Phosphorylation regulates the delivery of MHC class II invariant chain complexes to antigen processing compartments.
AuthorsAnderson HA, Roche PA
JournalJ Immunol
PubMed ID9590232
'Transport of newly synthesized MHC class II glycoproteins to endosomal Ag processing compartments is mediated by their association with the invariant chain (Ii). Targeting to these compartments is dependent upon recognition of leucine-based endo. somal/lysosomal targeting motifs in the Ii cytosolic domain. Ii, like many molecules that contain leucine- based ... More
Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene.
AuthorsMetes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA
JournalBlood
PubMed ID9516136
'Human natural killer (NK) cells were thought to express only FcgammaRIIIA (CD16), but recent reports have indicated that NK cells also express a second type of FcgammaR, ie, FcgammaRII (CD32). We have isolated, cloned, and sequenced full-length cDNAs of FcgammaRII from NK cells derived from several normal individuals that may ... More
Regulated expression of the Shiga toxin B gene induces apoptosis in mammalian fibroblastic cells.
AuthorsNakagawa I, Nakata M, Kawabata S, Hamada S
JournalMol Microbiol
PubMed ID10510233
'Shiga toxins (Stxs) produced by enterohaemorrhagic Escherichia coli may induce colonic ulceration, bloody diarrhoea and acute renal failure. The A subunit (StxA) is known to inhibit protein synthesis, whereas the B subunits (StxB) bind to Gb3 on the cell surface. However, the mechanisms by which Stxs kill target cells remain ... More
Mouse transporter protein, a membrane protein that regulates cellular multidrug resistance, is localized to lysosomes.
AuthorsCabrita MA, Hobman TC, Hogue DL, King KM, Cass CE
JournalCancer Res
PubMed ID10519401
'Mouse transporter protein (MTP), a small, highly conserved mammalian intracellular membrane protein with four putative transmembrane domains, has been implicated in the transport of nucleosides and/or related molecules across intracellular membranes. The production of recombinant MTP in Saccharomyces cerevisiae alters sensitivity of yeast cells to a heterogeneous group of compounds ... More
Megakaryoblastic leukemia factor-1 transduces cytoskeletal signals and induces smooth muscle cell differentiation from undifferentiated embryonic stem cells.
AuthorsDu KL, Chen M, Li J, Lepore JJ, Mericko P, Parmacek MS,
JournalJ Biol Chem
PubMed ID14970199
'The SAP domain transcription factor myocardin plays a critical role in the transcriptional program regulating smooth muscle cell differentiation. In this report, we describe the capacity of myocardin to physically associate with megakaryoblastic leukemia factor-1 (MKL1) and characterize the function of MKL1 in smooth muscle cells (SMCs). The MKL1 gene ... More
Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes.
Authors Zaccolo Manuela; Pozzan Tullio;
JournalScience
PubMed ID11872839
'The second messenger cyclic adenosine monophosphate (cAMP) is the most important modulator of sympathetic control over cardiac contractility. In cardiac myocytes and many other cell types, however, cAMP transduces the signal generated upon stimulation of various receptors and activates different cellular functions, raising the issue of how specificity can be ... More
Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1.
AuthorsFurusawa T, Moribe H, Kondoh H, Higashi Y
JournalMol Cell Biol
PubMed ID10567582
' deltaEF1, a representative of the zinc finger-homeodomain protein family, is a transcriptional repressor which binds E2-box (CACCTG) and related sequences and counteracts the activators through transrepression mechanisms. It has been shown that the N-proximal region of the protein is involved in the transrepression. Here we demonstrate that deltaEF1 has ... More
Protracted course of Krabbe disease in an adult patient bearing a novel mutation
'BACKGROUND: Krabbe disease, or globoid cell leukodystrophy, is an autosomal recessive disorder caused by the deficiency of galactocerebrosidase (GALC) activity. Although most cases are diagnosed in infancy and show a fatal outcome in childhood, adult patients have been identified, showing progressive spastic hemiparesis to tetraparesis, followed by optic atrophy, dementia, ... More
Cloning and characterization of mouse vascular adhesion protein-1 reveals a novel molecule with enzymatic activity.
AuthorsBono P, Salmi M, Smith DJ, Jalkanen S
JournalJ Immunol
PubMed ID9605161
'Human vascular adhesion protein-1 (VAP-1) is a sialylated endothelial cell adhesion molecule mediating the initial L-selectin-independent interactions between lymphocytes and endothelial cells in man. In this work we cloned and characterized mouse VAP-1 (mVAP-1) and produced an anti-mVAP-1 mAb against a recombinant mVAP-1 fusion protein. The isolated cDNA encodes a ... More
A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA.
AuthorsTronchere H, Wang J, Fu XD
JournalNature
PubMed ID9237760
'Recognition of a functional 3'' splice site in pre-mRNA splicing requires a heterodimer of the proteins U2AF65/U2AF35. U2AF65 binds to RNA at the polypyrimidine tract, whereas U2AF35 is thought to interact through its arginine/serine-rich (RS) domain with other RS-domain-containing factors bound at the 5'' splice site, assembled in splicing enhancer ... More
HIV coreceptor downregulation as antiviral principle: SDF-1alpha-dependent internalization of the chemokine receptor CXCR4 contributes to inhibition of HIV replication.
AuthorsAmara A, Gall SL, Schwartz O, Salamero J, Montes M, Loetscher P, Baggiolini M, Virelizier JL, Arenzana-Seisdedos F
JournalJ Exp Med
PubMed ID9207008
'Ligation of CCR5 by the CC chemokines RANTES, MIP-1alpha or MIP-1beta, and of CXCR4 by the CXC chemokine SDF-1alpha, profoundly inhibits the replication of HIV strains that use these coreceptors for entry into CD4(+) T lymphocytes. The mechanism of entry inhibition is not known. We found a rapid and extensive ... More
Amphiphysin II (SH3P9; BIN1), a member of the amphiphysin/Rvs family, is concentrated in the cortical cytomatrix of axon initial segments and nodes of ranvier in brain and around T tubules in skeletal muscle.
AuthorsButler MH, David C, Ochoa GC, Freyberg Z, Daniell L, Grabs D, Cremona O, De Camilli P
JournalJ Cell Biol
PubMed ID9182667
'Amphiphysin (amphiphysin I), a dominant autoantigen in paraneoplastic Stiff-man syndrome, is a neuronal protein highly concentrated in nerve terminals, where it has a putative role in endocytosis. The yeast homologue of amphiphysin, Rvs167, has pleiotropic functions, including a role in endocytosis and in actin dynamics, suggesting that amphiphysin may also ... More
Protein binding and signaling properties of RIN1 suggest a unique effector function.
AuthorsHan L, Wong D, Dhaka A, Afar D, White M, Xie W, Herschman H, Witte O, Colicelli J
JournalProc Natl Acad Sci U S A
PubMed ID9144171
'Human RIN1 was first characterized as a RAS binding protein based on the properties of its carboxyl-terminal domain. We now show that full- length RIN1 interacts with activated RAS in mammalian cells and defines a minimum region of 434 aa required for efficient RAS binding. RIN1 interacts with the effector ... More
Transcriptional Regulation of the Pituitary Vasopressin V1b Receptor Involves a GAGA-binding Protein.
'The role of CT repeats (inverted GAGA box) in the rat vasopressin V1b receptor (V1bR) promoter in the transcriptional regulation of this gene was studied in H32 hypothalamic cells, which express endogenous V1bR. Transfection of a 2.5-kb V1bR fragment (2161 bp upstream and 377 bp downstream of the proximal transcriptional ... More
Urokinase-type plasminogen activator receptor (CD87) is a ligand for integrins and mediates cell-cell interaction.
Authors Tarui T; Mazar A P; Cines D B; Takada Y;
JournalJ Biol Chem
PubMed ID11053440
'Binding of urokinase-type plasminogen activator (uPA) to its receptor (uPAR/CD87) regulates cellular adhesion, migration, and tumor cell invasion. However, it is unclear how glycosyl phosphatidylinositol-anchored uPAR, which lacks a transmembrane structure, mediates signal transduction. It has been proposed that uPAR forms cis-interactions with integrins as an associated protein and thereby ... More
SAP90 binds and clusters kainate receptors causing incomplete desensitization.
'The mechanism of kainate receptor targeting and clustering is still unresolved. Here, we demonstrate that members of the SAP90/PSD-95 family colocalize and associate with kainate receptors. SAP90 and SAP102 coimmunoprecipitate with both KA2 and GluR6, but only SAP97 coimmunoprecipitates with GluR6. Similar to NMDA receptors, GluR6 clustering is mediated by ... More
Dermo-1, a Multifunctional Basic Helix-Loop-Helix Protein, Represses MyoD Transactivation via the HLH Domain, MEF2 Interaction, and Chromatin Deacetylation.
Authors Gong Xue Q; Li Li;
JournalJ Biol Chem
PubMed ID11809751
'Dermo-1 is a multifunctional basic helix-loop-helix (bHLH) transcription factor that has been shown to be a potent negative regulator for gene transcription and apoptosis. To understand the molecular mechanisms that mediate the function of Dermo-1, we generated a series of Dermo-1 mutants and used a MyoD-mediated transcriptional activation model to ... More
15-Lipoxygenase metabolism of 2-arachidonylglycerol. Generation of a peroxisome proliferator-activated receptor alpha agonist.
Authors Kozak Kevin R; Gupta Rajnish A; Moody John S; Ji Chuan; Boeglin William E; DuBois Raymond N; Brash Alan R; Marnett Lawrence J;
JournalJ Biol Chem
PubMed ID11956198
'The recent demonstrations that cyclooxygenase-2 and leukocyte-type 12-lipoxygenase (LOX) efficiently oxygenate 2-arachidonylglycerol (2-AG) prompted an investigation into related oxygenases capable of metabolizing this endogenous cannabinoid receptor ligand. We evaluated the ability of six LOXs to catalyze the hydroperoxidation of 2-AG. Soybean 15-LOX, rabbit reticulocyte 15-LOX, human 15-LOX-1, and human 15-LOX-2 ... More
Identification of the novel K15 gene at the rightmost end of the Kaposi's sarcoma-associated herpesvirus genome.
AuthorsChoi JK, Lee BS, Shim SN, Li M, Jung JU.
JournalJ Virol
PubMed ID10590133
'Kaposi''s sarcoma-associated herpesvirus (KSHV) encodes a distinct open reading frame called K15 at a position equivalent to the gene encoding LMP2A of Epstein-Barr virus (EBV). K15 isolates from body cavity-based lymphoma (BCBL) cells exhibited a dramatic sequence variation and a complex splicing pattern. However, all K15 alleles are organized similarly ... More
Upstream organization of and multiple transcripts from the human folylpoly-gamma-glutamate synthetase gene.
AuthorsFreemantle SJ, Taylor SM, Krystal G, Moran RG
JournalJ Biol Chem
PubMed ID7721888
'Folylpoly-gamma-glutamate synthetase (FPGS) is essential for the survival of proliferating mammalian cells and central to the action of all classical folate antimetabolites. We report the isolation of cDNAs corresponding to the 5'' ends of FPGS mRNA from both human and hamster cells which include a start codon upstream of and ... More
Activation and inhibition of G protein-coupled inwardly rectifying potassium (Kir3) channels by G protein beta gamma subunits.
AuthorsLei Q, Jones MB, Talley EM, Schrier AD, McIntire WE, Garrison JC, Bayliss DA
JournalProc Natl Acad Sci U S A
PubMed ID10944236
'G protein-coupled inwardly rectifying potassium (GIRK) channels can be activated or inhibited by different classes of receptors, suggesting a role for G proteins in determining signaling specificity. Because G protein betagamma subunits containing either beta1 or beta2 with multiple Ggamma subunits activate GIRK channels, we hypothesized that specificity might be ... More
The ebola virus VP35 protein functions as a type I IFN antagonist
AuthorsBasler CF, Wang X, Muhlberger E, Volchkov V, Paragas J, Klenk HD, Garcia-Sastre A, Palese P
JournalProc Natl Acad Sci U S A
PubMed ID11027311
'An assay has been developed that allows the identification of molecules that function as type I IFN antagonists. Using this assay, we have identified an Ebola virus-encoded inhibitor of the type I IFN response, the Ebola virus VP35 protein. The assay relies on the properties of an influenza virus mutant, ... More
A carboxyl-terminal PDZ-interacting domain of scavenger receptor B, type I is essential for cell surface expression in liver.
Authors Silver David L;
JournalJ Biol Chem
PubMed ID12119305
'Scavenger receptor B, type I (SR-BI) was recently shown to interact with a PDZ domain-containing protein, PDZK1 (CLAMP/Diphor-1/CAP70/NaPi-Cap1), but the importance of this interaction in vivo in terms of SR-BI function has not been determined. In an effort to elucidate the role of this interaction in vivo, the PDZK1-interacting domain ... More
Inactivating mutations and overexpression of BCL10, a caspase recruitment domain-containing gene, in MALT lymphoma with t(1;14)(p22;q32).
AuthorsZhang Q, Siebert R, Yan M, Hinzmann B, Cui X, Xue L, Rakestraw KM, Naeve CW, Beckmann G, Weisenburger DD, Sanger WG, Nowotny H, Vesely M, Callet-Bauchu E, Salles G, Dixit VM, Rosenthal A, Schlegelberger B, Morris SW
JournalNature Genetics
PubMed ID10319863
'Mucosa-associated lymphoid tissue (MALT) lymphomas most frequently involve the gastrointestinal tract and are the most common subset of extranodal non-Hodgkin lymphoma (NHL). Here we describe overexpression of BCL10, a novel apoptotic signalling gene that encodes an amino-terminal caspase recruitment domain (CARD), in MALT lymphomas due to the recurrent t(1;14)(p22;q32). BCL10 ... More
A receptor for phosphatidylserine-specific clearance of apoptotic cells
AuthorsFadok VA, Bratton DL, Rose DM, Pearson A, Ezekewitz RA, Henson PM
JournalScience
PubMed ID10811223
'The culmination of apoptosis in vivo is phagocytosis of cellular corpses. During apoptosis, the asymmetry of plasma membrane phospholipids is lost, which exposes phosphatidylserine externally. During apoptosis, the asymmetry of plasma membrane phospholipids is lost, which exposes phosphatidylserine externally. The phagocytosis of apoptotic cells can be inhibited stereospecifically by ... More
The beta subunit determines the ion selectivity of the GABAA receptor.
Authors Jensen Marianne L; Timmermann Daniel B; Johansen Tina H; Schousboe Arne; Varming Thomas; Ahring Philip K;
JournalJ Biol Chem
PubMed ID12177063
'The gamma-aminobutyric acid, type A (GABA(A)) receptor is a chloride-conducting receptor composed of alpha, beta, and gamma subunits assembled in a pentameric structure forming a central pore. Each subunit has a large extracellular agonist binding domain and four transmembrane domains (M1-M4), with the second transmembrane (M2) domain lining the pore. ... More
Altered kinetics and benzodiazepine sensitivity of a GABAA receptor subunit mutation [gamma 2(R43Q)] found in human epilepsy.
Authors Bowser David N.; Wagner David A.; Czajkowski Cynthia; Cromer Brett A.; Parker Michael W.; Wallace Robyn H.; Harkin Louise A.; Mulley John C.; Marini Carla; Berkovic Samuel F.; Williams David A.; Jones Mathew V.; Petrou Steven;
JournalProc Natl Acad Sci U S A
PubMed ID12415111
'The gamma-aminobutyric acid type A (GABA(A)) receptor mediates fast inhibitory synaptic transmission in the CNS. Dysfunction of the GABA(A) receptor would be expected to cause neuronal hyperexcitability, a phenomenon linked with epileptogenesis. ' ... More
Regulation of cell death protease caspase-9 by phosphorylation.
AuthorsCardone MH, Roy N, Stennicke HR, Salvesen GS, Franke TF, Stanbridge E, Frisch S, Reed JC
JournalScience
PubMed ID9812896
'Caspases are intracellular proteases that function as initiators and effectors of apoptosis. The kinase Akt and p21-Ras, an Akt activator, induced phosphorylation of pro-caspase-9 (pro-Casp9) in cells. Cytochrome c-induced proteolytic processing of pro-Casp9 was defective in cytosolic extracts from cells expressing either active Ras or Akt. Akt phosphorylated recombinant Casp9 ... More
The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells.
AuthorsZheng W, Flavell RA
JournalCell
PubMed ID9160750
'CD4 T cells potentiate the inflammatory or humoral immune response through the action of Th1 and Th2 cells, respectively. The molecular basis of the differentiation of these cells from naive T cell precursors is, however, unclear. We found that GATA-3 was selectively expressed in Th2 cells. GATA-3 is expressed at ... More
TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family.
AuthorsNakayama J, Saito M, Nakamura H, Matsuura A, Ishikawa F
JournalCell
PubMed ID9118230
'We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240 and p230 ... More
Bifocal is a downstream target of the Ste20-like serine/threonine kinase misshapen in regulating photoreceptor growth cone targeting in Drosophila.
AuthorsRuan W, Long H, Vuong DH, Rao Y,
JournalNeuron
PubMed ID12467587
'Misshapen (Msn) has been proposed to shut down Drosophila photoreceptor (R cell) growth cone motility in response to targeting signals linked by the SH2/SH3 adaptor protein Dock. Here, we show that Bifocal (Bif), a putative cytoskeletal regulator, is a component of the Msn pathway for regulating R cell growth cone ... More
The protein SET binds the neuronal Cdk5 activator p35nck5a and modulates Cdk5/p35nck5a activity.
Authors Qu Dianbo; Li Qing; Lim Hui-Ying; Cheung Nam Sang; Li Rong; Wang Jerry H; Qi Robert Z;
JournalJ Biol Chem
PubMed ID11741927
'The neuronal Cdk5 kinase is composed of the catalytic subunit Cdk5 and the activator protein p35(nck5a) or its isoform, p39(nck5ai). To identify novel p35(nck5a)- and p39(nck5ai)-binding proteins, fragments of p35(nck5a) and p39(nck5ai) were utilized in affinity isolation of binding proteins from rat brain homogenates, and the isolated proteins were identified ... More
The human acid alpha-glucosidase gene is a novel target of the Notch-1/Hes-1 signaling pathway.
AuthorsYan B, Raben N, Plotz P.
JournalJ Biol Chem
PubMed ID12065598
'Acid alpha-glucosidase (GAA) is a lysosomal enzyme that degrades glycogen. A deficiency of GAA is responsible for a recessively inherited myopathy and cardiomyopathy, glycogenosis type II. Previously, we identified an intronic repressor element in the GAA gene and demonstrated that Hes-1, a basic helix-loop-helix factor, binds to a C class ... More
A modified screening method for pcDNA-1 expression libraries which is applicable to both surface and intracellular antigens. Cloning of a colon carcinoma antigen.
AuthorsGrimm T, Johnson JP
JournalJ Immunol Methods
PubMed ID7594630
'A modification of the COS cell transient expression system for isolating cDNAs encoding antibody defined molecules was used to clone the carcinoma antigen defined by monoclonal antibody CORC-6. Bacteria were transformed with cDNA prepared from colon carcinoma cells and immediately divided into aliquots containing approximately 500 independent clones. The aliquots ... More
Selective reconstitution and recovery of functional gamma-secretase complex on budded baculovirus particles.
AuthorsHayashi I, Urano Y, Fukuda R, Isoo N, Kodama T, Hamakubo T, Tomita T, Iwatsubo T,
JournalJ Biol Chem
PubMed ID15215237
'In vitro reconstitution of functions of membrane proteins is often hampered by aggregation, misfolding, or lack of post-translational modifications of the proteins attributable to overexpression. To overcome this technical obstacle, we have developed a method to express multimeric integral membrane proteins in extracellular (budded) baculovirus particles that are released from ... More
Cloning of a new gene encoding an antigen recognized by melanoma- specific HLA-A24-restricted tumor-infiltrating lymphocytes.
AuthorsRobbins PF, el-Gamil M, Li YF, Topalian SL, Rivoltini L, Sakaguchi K, Appella E, Kawakami Y, Rosenberg SA
JournalJ Immunol
PubMed ID7751637
'The role of tumor-specific T cells in mediating the regression of metastatic melanoma has been suggested by the clinical response of patients to treatment with tumor-infiltrating lymphocytes (TIL). A number of Ags recognized by class I-restricted melanoma-specific T cells have recently been isolated, raising the hope that this will lead ... More
Signal transduction and ligand specificity of the human monocyte chemoattractant protein-1 receptor in transfected embryonic kidney cells.
Authors Myers S J; Wong L M; Charo I F;
JournalJ Biol Chem
PubMed ID7890708
'We have examined the ligand specificity and signal transduction pathways of a recently cloned receptor for monocyte chemoattractant protein-1 (MCP-1). In human 293 cells stably transfected with the MCP-1 receptor, MCP-1 bound specifically with high affinity (Kd = 260 pM) and induced a rapid mobilization of calcium from intracellular stores. ... More
Nitric Oxide-induced Apoptosis in RAW 264.7 Macrophages Is Mediated by Endoplasmic Reticulum Stress Pathway Involving ATF6 and CHOP.
Authors Gotoh Tomomi; Oyadomari Seiichi; Mori Kazutoshi; Mori Masataka;
JournalJ Biol Chem
PubMed ID11805088
'Excess nitric oxide (NO) induces apoptosis in some cell types including macrophages; however, the cascade of NO-mediated apoptosis is not fully understood. We investigated the initial steps of NO-mediated apoptosis in mouse macrophage-like RAW 264.7 cells. When cells were treated with bacterial lipopolysaccharide (LPS) plus interferon-gamma (IFN-gamma), NO-mediated apoptosis occurred. ... More
Post-translational processing and renal expression of mouse Indian hedgehog.
AuthorsValentini RP, Brookhiser WT, Park J, Yang T, Briggs J, Dressler G, Holzman LB
JournalJ Biol Chem
PubMed ID9079674
'The full-length mouse Indian hedgehog (Ihh) cDNA was cloned from an embryonic 17.5-day kidney library and was used to study the post- translational processing of the peptide and temporal and spatial expression of the transcript. Sequence analysis predicted two putative translation initiation sites. Ihh translation was initiated at both initiation ... More
Insertion of the adenoviral E3 region into a recombinant viral vector prevents antiviral humoral and cellular immune responses and permits long-term gene expression.
AuthorsIlan Y, Droguett G, Chowdhury NR, Li Y, Sengupta K, Thummala NR, Davidson A, Chowdhury JR, Horwitz MS
JournalProc Natl Acad Sci U S A
PubMed ID9122239
'Recombinant adenoviruses (Ads) are highly efficient at transferring foreign genes to the liver in vivo; however, the duration of gene expression is limited by the host antiviral immune response, which prevents expression upon readministration of the virus. To test whether overexpression of the immunomodulatory products of the early Ad genome ... More