A flow-cytometric gram-staining technique for milk-associated bacteria.
AuthorsHolm C, Jespersen L
JournalAppl Environ Microbiol
PubMed ID12732558
'A Gram-staining technique combining staining with two fluorescent stains, Oregon Green-conjugated wheat germ agglutinin (WGA) and hexidium iodide (HI) followed by flow-cytometric detection is described. WGA stains gram-positive bacteria while HI binds to the DNA of all bacteria after permeabilization by EDTA and incubation at 50 degrees C for 15 ... More
Loss of abaxial leaf epicuticular wax in Medicago truncatula irg1/palm1 mutants results in reduced spore differentiation of anthracnose and nonhost rust pathogens.
AuthorsUppalapati SR, Ishiga Y, Doraiswamy V, Bedair M, Mittal S, Chen J, Nakashima J, Tang Y, Tadege M, Ratet P, Chen R, Schultheiss H, Mysore KS,
JournalPlant Cell
PubMed ID22294617
'To identify genes that confer nonhost resistance to biotrophic fungal pathogens, we did a forward-genetics screen using Medicago truncatula Tnt1 retrotransposon insertion lines. From this screen, we identified an inhibitor of rust germ tube differentation1 (irg1) mutant that failed to promote preinfection structure differentiation of two rust pathogens, Phakopsora pachyrhizi ... More
Development of a quantitative, cell-based, high-content screening assay for epidermal growth factor receptor modulators.
AuthorsWang J, Xie X,
JournalActa Pharmacol Sin
PubMed ID17883960
'AIM: To develop a robust, cell-based, high-content screening (HCS) assay based on receptor internalization for the identification of novel modulators of the epidermal growth factor receptor (EGFR). METHODS: Agonist-induced receptor internalization is part of the signaling cascade of EGFR. Fluorescent-tagged epidermal growth factor (EGF) was used to visualize the internalized ... More
Sympathetic alpha(2)-adrenoceptors prevent cardiac hypertrophy and fibrosis in mice at baseline but not after chronic pressure overload.
'alpha(2)-Adrenoceptors modulate cardiovascular function by vasoconstriction or dilatation, by central inhibition of sympathetic activity, or by feedback inhibition of norepinephrine release from sympathetic neurons. Despite detailed knowledge about subtype-specific functions of alpha(2)-receptors, the relative contributions of sympathetic vs. non-sympathetic receptors involved in these cardiovascular effects have not been identified. The ... More
Murine hyaluronidase 2 deficiency results in extracellular hyaluronan accumulation and severe cardiopulmonary dysfunction.
Hyaluronidase (HYAL) 2 is a membrane-anchored protein that is proposed to hydrolyze hyaluronan (HA) to smaller fragments that are internalized for breakdown. Initial studies of a Hyal2 knock-out (KO) mouse revealed a mild phenotype with high serum HA, supporting a role for HYAL2 in HA breakdown. We now describe a ... More
Normal lactational environment restores cardiomyocyte number after uteroplacental insufficiency: implications for the preterm neonate.
AuthorsBlack MJ, Siebel AL, Gezmish O, Moritz KM, Wlodek ME,
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID22403799
A reduced complement of cardiomyocytes in early life can adversely affect life-long cardiac functional reserve. In the present study, using a cross-fostering approach in rats, we examined the contributions of the prenatal and postnatal environments in the programming of cardiomyocyte growth. Rat dams underwent either bilateral uterine vessel ligation (Restricted) ... More
Characterization of the poly-ß-1,6-N-acetylglucosamine polysaccharide component of Burkholderia biofilms.
AuthorsYakandawala N, Gawande PV, LoVetri K, Cardona ST, Romeo T, Nitz M, Madhyastha S,
JournalAppl Environ Microbiol
PubMed ID21984237
We demonstrated the production of poly-ß-1,6-N-acetylglucosamine (PNAG) polysaccharide in the biofilms of Burkholderia multivorans, Burkholderia vietnamiensis, Burkholderia ambifaria, Burkholderia cepacia, and Burkholderia cenocepacia using an immunoblot assay for PNAG. These results were confirmed by further studies, which showed that the PNAG hydrolase, dispersin B, eliminated immunoreactivity of extracts from the ... More
Alcohol exposure during late gestation adversely affects myocardial development with implications for postnatal cardiac function.
Prenatal exposure to high levels of ethanol is associated with cardiac malformations, but the effects of lower levels of exposure on the heart are unclear. Our aim was to investigate the effects of daily exposure to ethanol during late gestation, when cardiomyocytes are undergoing maturation, on the developing myocardium. Pregnant ... More
Cardiac remodelling as a result of pre-term birth: implications for future cardiovascular disease.
AuthorsBensley JG, Stacy VK, De Matteo R, Harding R, Black MJ,
JournalEur Heart J
PubMed ID20453064
Pre-term birth affects 10-12% of live births and occurs when the myocardium is still developing; therefore, the final structure of the myocardium could be altered. We hypothesized that, in response to pre-term birth, structural remodelling occurs within the myocardium which enables the immature heart muscle to adapt to the haemodynamic ... More
Complex formation among murine cytomegalovirus US22 proteins encoded by genes M139, M140, and M141.
The murine cytomegalovirus (MCMV) proteins encoded by US22 genes M139, M140, and M141 function, at least in part, to regulate replication of this virus in macrophages. Mutant MCMV having one or more of these genes deleted replicates poorly in macrophages in culture and in the macrophage-dense environment of the spleen. ... More
PI3Kα-regulated gelsolin activity is a critical determinant of cardiac cytoskeletal remodeling and heart disease.
Therapeutic Benefit and Gene Network Regulation by Combined Gene Transfer of Apelin, FGF2, and SERCA2a into Ischemic Heart.
Authors
JournalMol Ther
PubMed ID29249393
Synthesis and functionalization of monodisperse near-ultraviolet and visible excitable multifunctional Eu(3+), Bi(3+):REVO4 nanophosphors for bioimaging and biosensing applications.
Authors
JournalNanoscale
PubMed ID27253384
Structural evidence for a new elaborate 3D-organization of the cardiomyocyte lateral membrane in adult mammalian cardiac tissues.
Authors
JournalCardiovasc Res
PubMed ID30329023
Inhibition of FPR2 impaired leukocytes recruitment and elicited non-resolving inflammation in acute heart failure.
Authors
JournalPharmacol Res
PubMed ID31216426
Galectin-1 Protein Therapy Prevents Pathology and Improves Muscle Function in the mdx Mouse Model of Duchenne Muscular Dystrophy.
Authors
JournalMol Ther
PubMed ID26050991
Bi-allelic Truncating Mutations in TANGO2 Cause Infancy-Onset Recurrent Metabolic Crises with Encephalocardiomyopathy.
Authors
JournalAm J Hum Genet
PubMed ID26805782
Abrogation of Nrf2 impairs antioxidant signaling and promotes atrial hypertrophy in response to high-intensity exercise stress.
Authors
JournalJ Transl Med
PubMed ID27048381
Social motility of biofilm-like microcolonies in a gliding bacterium.