WesternBreeze™ Blocker/Diluent (Part A and B)

El bloqueador/diluyente WesternBreeze® (parte A y B) es un sistema optimizado de bloqueadores y diluyentes de anticuerpos primarios fácil deMás información

Número de catálogo	Cantidad
WB7050	80 mL

Número de catálogo WB7050

Precio (MXN)

Cantidad:

80 mL

El bloqueador/diluyente WesternBreeze® (parte A y B) es un sistema optimizado de bloqueadores y diluyentes de anticuerpos primarios fácil de usar que produce detección mediante Western blot de bajo fondo y señal alta en membranas de nitrocelulosa (NC) y difluoruro de polivinilideno (PVDF). Suficientes reactivos para 20 miniblots.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

TampónTampones de inmunotransferencia

Cantidad80 mL

Membrane CompatibilityNitrocelulosa, PVDF

Línea de productosWesternBreeze

Tipo de productoBloqueador y diluyente

Unit Size80 mL

Contenido y almacenamiento

80 ml de bloqueador/diluyente (parte A);
80 ml de bloqueador/diluyente (parte B).

Almacenar a +4 °C.

Preguntas frecuentes

Why is the actual band size on a western blot different from the predicted size of the protein?

Western blotting is based on the separation of proteins by their size on a gel. However, migration of proteins through the gel matrix is also affected by other factors, which may cause the observed band size to be different from the predicted size.

Common causes are:
-Post-translational modification; for example phosphorylation and glycosylation increase the size of the protein
-Post-translation cleavage; many proteins are synthesized as precursor proteins, and then cleaved to give the active form
-Multimers, for example dimerization of a protein. This is usually prevented under reducing conditions, although strong interactions can result in the appearance of higher bands
-Splice variants; alternative splicing may result in different sized proteins being produced from the same gene
-Relative charge; the composition of amino acids (charged vs. non-charged)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chemiluminescent Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)
- Cat. No. WB7003 (Antibody Wash)

Note: Cat. Nos. WB7001 and WB7002 may be ordered by clicking the Quick Order button located at the top right of any page on our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I purchase the Blocker/Diluent and Antibody Wash solutions from the WesternBreeze Chromogenic Detection kits as standalone products?

Yes, you may purchase them as standalone products using the Cat. Nos. listed below:

- Cat. No. WB7003 (Antibody Wash)
- Cat. No. WB7001 (Blocker/Diluent A)
- Cat. No. WB7002 (Blocker/Diluent B)
- Cat. No. WB7050 (Combo pack containing Blocker/Diluents A & B)

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.