WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase. Non-fading purple precipitates develop at the protein bands on the membrane. WesternBreeze® chromogenic offers:
• Clear background • High sensitivity-low picogram levels detectable • High specificity • One simple protocol-no optimization required
For Research Use Only. Not for use in diagnostic procedures.
AP (Alkaline Phosphatase) Substrate
Contents & Storage
The WesternBreeze™ Chromogenic Kits include blocking solutions, primary antibody diluent, ready-to-use secondary antibody solution (anti-mouse, anti-rabbit or anti-goat), ready-to-use chromogenic substrate, wash solutions, and incubation trays. Each kit contains complete reagents for 20 mini-blots. Store the kits at +4°C. Guaranteed stable for 6 months when properly stored.
Blot developed with WesternBreeze® Chromogenic Kit (anti-rabbit)