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Invitrogen™

WesternBreeze™ Chemiluminescent Kit, anti-rabbit

WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directlyRead more
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Catalog NumberQuantity
WB71061 kit
Catalog number WB7106
Price (USD)
654.65
Online exclusive
682.00
Save 27.35
1 kit
Quantity:
1 kit
Price (USD)
654.65
Online exclusive
682.00
Save 27.35
1 kit
WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use CDP-Star® chemiluminescent substrate for alkaline phosphatase. Protein bands can be captured either by X-ray film or CDP-Star® compatible imaging system. WesternBreeze® Chemiluminescent Kit offers:

• High specificity, clean background
• Ultra-sensitivity-femtogram levels detectable
• Long-lasting signals-up to 5 days
• Results in less than 3 hours
• Permanent photographic image
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cross ReactivityRabbit
Quantity1 kit
ReactivityRabbit
Shipping ConditionWet Ice
Substrate TypeAP (Alkaline Phosphatase) Substrate
Detection MethodChemiluminescence
Membrane CompatibilityNitrocellulose, PVDF
Product LineWesternBreeze
Product TypeWestern Blot Kit
Unit Size1 kit
Contents & Storage
The WesternBreeze™ Chemiluminescent Kits include blocking solutions, primary antibody diluent, ready-to-use secondary antibody solution (anti-mouse, anti-rabbit or anti-goat), ready-to-use chemiluminescent substrate, wash solutions, incubation trays, pre-cut filter papers, polyester sheet for even substrate development on the membrane. Each kit contains complete reagents for 20 blots. Store the kits at +4°C. Guaranteed stable for 6 months when properly stored.

Citations & References (2)

Citations & References
Abstract
Hypomorphic apolipoprotein E mice: a new model of conditional gene repair to examine apolipoprotein E-mediated metabolism.
Authors: Raffai Robert L; Weisgraber Karl H;
Journal:J Biol Chem
PubMed ID:11792702
'In creating an allelic variant of mouse Apoe designed to resemble human apolipoprotein E4 (apoE4), we generated hypomorphic apoE (hypoE) mice that express only approximately 5% of normal apoE mRNA levels in all tissues. Insertion of a neo cassette flanked by loxP sites in the third intron of Apoe reduced ... More
An iron-regulated ferric reductase associated with the absorption of dietary iron.
Authors:McKie AT, Barrow D, Latunde-Dada GO, Rolfs A, Sager G, Mudaly E, Mudaly M, Richardson C, Barlow D, Bomford A, Peters TJ, Raja KB, Shirali S, Hediger MA, Farzaneh F, Simpson RJ.
Journal:Science
PubMed ID:11230685
The ability of intestinal mucosa to absorb dietary ferric iron is attributed to the presence of a brush-border membrane reductase activity that displays adaptive responses to iron status. We have isolated a complementary DNA, Dcytb (for duodenal cytochrome b), which encoded a putative plasma membrane di-heme protein in mouse duodenal ... More