WesternBreeze® Chemiluminescent Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use CDP-Star® chemiluminescent substrate for alkaline phosphatase. Protein bands can be captured either by X-ray film or CDP-Star® compatible imaging system. WesternBreeze® Chemiluminescent Kit offers:
• High specificity, clean background • Ultra-sensitivity-femtogram levels detectable • Long-lasting signals-up to 5 days • Results in less than 3 hours • Permanent photographic image
For Research Use Only. Not for use in diagnostic procedures.
AP (Alkaline Phosphatase) Substrate
Protein Detection Kit
Contents & Storage
The WesternBreeze™ Chemiluminescent Kits include blocking solutions, primary antibody diluent, ready-to-use secondary antibody solution (anti-mouse, anti-rabbit or anti-goat), ready-to-use chemiluminescent substrate, wash solutions, incubation trays, pre-cut filter papers, polyester sheet for even substrate development on the membrane. Each kit contains complete reagents for 20 blots. Store the kits at +4°C. Guaranteed stable for 6 months when properly stored.
Blot developed with WesternBreeze® Chemiluminescent Kit (anti-rabbit)-10 second exposure