Cocaine-induced dendritic spine formation in D1 and D2 dopamine receptor-containing medium spiny neurons in nucleus accumbens.
AuthorsLee KW, Kim Y, Kim AM, Helmin K, Nairn AC, Greengard P
JournalProc Natl Acad Sci U S A
PubMed ID16492766
'Psychostimulant-induced alteration of dendritic spines on dopaminoceptive neurons in nucleus accumbens (NAcc) has been hypothesized as an adaptive neuronal response that is linked to long-lasting addictive behaviors. NAcc is largely composed of two distinct subpopulations of medium-sized spiny neurons expressing high levels of either dopamine D1 or D2 receptors. In ... More
The second extracellular loop of CCR5 contains the dominant epitopes for highly potent anti-human immunodeficiency virus monoclonal antibodies.
AuthorsZhang J, Rao E, Dioszegi M, Kondru R, DeRosier A, Chan E, Schwoerer S, Cammack N, Brandt M, Sankuratri S, Ji C
JournalAntimicrob Agents Chemother
PubMed ID17242138
'Six mouse anti-human CCR5 monoclonal antibodies (mAbs) that showed potent antiviral activities were identified from over 26,000 mouse hybridomas. The epitopes for these mAbs were determined by using various CCR5 mutants, including CCR5/CCR2B chimeras. One mAb, ROAb13, was found to bind to a linear epitope in the N terminus of ... More
Co-localization of amyloid beta and tau pathology in Alzheimer's disease synaptosomes.
AuthorsFein JA, Sokolow S, Miller CA, Vinters HV, Yang F, Cole GM, Gylys KH,
JournalAm J Pathol
PubMed ID18467692
The amyloid cascade hypothesis proposes that amyloid beta (Abeta) pathology precedes and induces tau pathology, but the neuropathological connection between these two lesions has not been demonstrated. We examined the regional distribution and co-localization of Abeta and phosphorylated tau (p-tau) in synaptic terminals of Alzheimer's disease brains. To quantitatively examine ... More
TIM-mediated inhibition of HIV-1 release is antagonized by Nef but potentiated by SERINC proteins.
AuthorsLi M, Waheed AA, Yu J, Zeng C, Chen HY, Zheng YM, Feizpour A, Reinhard BM, Gummuluru S, Lin S, Freed EO, Liu SL
JournalProc Natl Acad Sci U S A
PubMed ID30842281
'The T cell Ig and mucin domain (TIM) proteins inhibit release of HIV-1 and other enveloped viruses by interacting with cell- and virion-associated phosphatidylserine (PS). Here, we show that the Nef proteins of HIV-1 and other lentiviruses antagonize TIM-mediated restriction. TIM-1 more potently inhibits the release of Nef-deficient relative to ... More
Dynamics and Differences in Systemic and Local Immune Responses After Vaccination With Inactivated and Live Commercial Vaccines and Subsequent Subclinical Infection With PRRS Virus.
AuthorsToman M, Celer V, Kavanová L, Levá L, Frolichova J, Ondrácková P, Kudlácková H, Nechvátalová K, Salat J, Faldyna M
JournalFront Immunol
PubMed ID31447829
'The goals of our study were to compare the immune response to different killed and modified live vaccines against PRRS virus and to monitor the antibody production and the cell mediated immunity both at the systemic and local level. In the experiment, we immunized four groups of piglets with two ... More
Arf6, JIP3, and dynein shape and mediate macropinocytosis.
AuthorsWilliamson CD, Donaldson JG
JournalMol Biol Cell
PubMed ID30969891
'Macropinocytosis is an actin-driven form of clathrin-independent endocytosis that generates an enlarged structure, the macropinosome. Although many studies focus on signaling molecules and phosphoinositides involved in initiating macropinocytosis, the commitment to forming a macropinosome and the handling of that membrane have not been studied in detail. Here we show in ... More
Characterization of the Myeloid Cell Populations' Resident in the Porcine Palatine Tonsil.
AuthorsSoldevila F, Edwards JC, Graham SP, Stevens LM, Crudgington B, Crooke HR, Werling D, Steinbach F
JournalFront Immunol
PubMed ID30158925
'The palatine tonsil is the portal of entry for food and air and is continuously subjected to environmental challenges, including pathogens, which use the tonsil and pharynx as a primary site of replication. In pigs, this includes the viruses causing porcine respiratory and reproductive syndrome, and classical and African swine ... More
Human cytomegalovirus-infected cells release extracellular vesicles that carry viral surface proteins.
AuthorsZicari S, Arakelyan A, Palomino RAÑ, Fitzgerald W, Vanpouille C, Lebedeva A, Schmitt A, Bomsel M, Britt W, Margolis L
JournalVirology
PubMed ID30165311
'Extracellular vesicles (EVs) released by virus-infected cells typically incorporate host and viral components inside the vesicles (cargo molecules). Here, we investigated if human cytomegalovirus (HCMV) proteins are incorporated in EV outer membrane released by HCMV-infected cells. We separated EVs from HCMV using an iodixanol step-gradient and found that the separated ... More
Gene expression and mutation-guided synthetic lethality eradicates proliferating and quiescent leukemia cells.
AuthorsNieborowska-Skorska M, Sullivan K, Dasgupta Y, Podszywalow-Bartnicka P, Hoser G, Maifrede S, Martinez E, Di Marcantonio D, Bolton-Gillespie E, Cramer-Morales K, Lee J, Li M, Slupianek A, Gritsyuk D, Cerny-Reiterer S, Seferynska I, Stoklosa T, Bullinger L, Zhao H, Gorbunova V, Piwocka K, Valent P, Civin CI, Muschen M, Dick JE, Wang JC, Bhatia S, Bhatia R, Eppert K, Minden MD, Sykes SM, Skorski T
JournalJ Clin Invest
PubMed ID28481221
'Quiescent and proliferating leukemia cells accumulate highly lethal DNA double-strand breaks that are repaired by 2 major mechanisms: BRCA-dependent homologous recombination and DNA-dependent protein kinase-mediated (DNA-PK-mediated) nonhomologous end-joining, whereas DNA repair pathways mediated by poly(ADP)ribose polymerase 1 (PARP1) serve as backups. Here we have designed a personalized medicine approach called ... More
IGH/MYC Translocation Associates with BRCA2 Deficiency and Synthetic Lethality to PARP1 Inhibitors.
AuthorsMaifrede S, Martin K, Podszywalow-Bartnicka P, Sullivan-Reed K, Langer SK, Nejati R, Dasgupta Y, Hulse M, Gritsyuk D, Nieborowska-Skorska M, Lupey-Green LN, Zhao H, Piwocka K, Wasik MA, Tempera I, Skorski T
JournalMol Cancer Res
PubMed ID28634224
Burkitt lymphoma/leukemia cells carry t(8;14)(q24;q32) chromosomal translocation encoding IGH/MYC, which results in the constitutive expression of the MYC oncogene. Here, it is demonstrated that untreated and cytarabine (AraC)-treated IGH/MYC-positive Burkitt lymphoma cells accumulate a high number of potentially lethal DNA double-strand breaks (DSB) and display low levels of the BRCA2 ... More
Human Semaphorin-4A drives Th2 responses by binding to receptor ILT-4.
AuthorsLu N, Li Y, Zhang Z, Xing J, Sun Y, Yao S, Chen L
JournalNat Commun
PubMed ID29467366
Semaphorin-4A (Sema4A) has been implicated in the co-stimulation of T cells and drives Th1 immune responses by binding to the receptor T-cell immunoglobulin and mucin domain protein 2 (Tim-2) in mice. Here we show that human, but not murine, Sema4A is preferentially expressed on antigen-presenting cells, and co-stimulates CD4 ... More
Pathologic Thr
AuthorsMoszczynski AJ, Strong W, Xu K, McKee A, Brown A, Strong MJ
JournalNeurology
PubMed ID29298849
To investigate whether chronic traumatic encephalopathy (CTE) and CTE with amyotrophic lateral sclerosis (CTE-ALS) exhibit features previously observed in other tauopathies of pathologic phosphorylation of microtubule-associated protein tau at Thr ... More
Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts.
AuthorsKuragano M, Uyeda TQP, Kamijo K, Murakami Y, Takahashi M
JournalMol Biol Cell
PubMed ID29467250
We demonstrated that myosin IIA and IIB are essential for the formation of transverse arcs and ventral stress fibers, respectively. Furthermore, we illustrated the roles of both isoforms in lamellar flattening and also raised the possibility that actin filaments in ventral stress fibers are in a stretched conformation. ... More
EWI-2 Inhibits Cell-Cell Fusion at the HIV-1 Virological Presynapse.
AuthorsWhitaker EE, Matheson NJ, Perlee S, Munson PB, Symeonides M, Thali M
JournalViruses
PubMed ID31757023
Cell-to-cell transfer of virus particles at the Env-dependent virological synapse (VS) is a highly efficient mode of HIV-1 transmission. While cell-cell fusion could be triggered at the VS, leading to the formation of syncytia and preventing exponential growth of the infected cell population, this is strongly inhibited by both viral ... More