MacMARCKS mutation blocks macrophage phagocytosis of zymosan.
AuthorsZhu Z, Bao Z, Li J
JournalJ Biol Chem
PubMed ID7629059
'A major protein kinase C substrate, MacMARCKS (F52, MPR), was examined for its role in phagocytosis. In macrophage-phagocytosing zymosan particles, MacMARCKS was concentrated around nascent phagosomes as detected by immunofluorescent microscopy. The effector domain of MacMARCKS contains the phosphorylation sites, a calmodulin binding site, as well as a putative actin ... More
Selective inhibition of IgG-mediated phagocytosis in gelsolin-deficient murine neutrophils.
AuthorsSerrander L, Skarman P, Rasmussen B, Witke W, Lew DP, Krause KH, Stendahl O, Nüsse O
JournalJ Immunol
PubMed ID10946270
'Phagocytosis and the microbicidal functions of neutrophils require dynamic changes of the actin cytoskeleton. We have investigated the role of gelsolin, a calcium-dependent actin severing and capping protein, in peripheral blood neutrophils from gelsolin-null (Gsn-) mice. The phagocytosis of complement opsonized yeast was only minimally affected. In contrast, phagocytosis of ... More
CFTR regulates phagosome acidification in macrophages and alters bactericidal activity.
AuthorsDi A, Brown ME, Deriy LV, Li C, Szeto FL, Chen Y, Huang P, Tong J, Naren AP, Bindokas V, Palfrey HC, Nelson DJ
JournalNat Cell Biol
PubMed ID16921366
'Acidification of phagosomes has been proposed to have a key role in the microbicidal function of phagocytes. Here, we show that in alveolar macrophages the cystic fibrosis transmembrane conductance regulator Cl- channel (CFTR) participates in phagosomal pH control and has bacterial killing capacity. Alveolar macrophages from Cftr-/- mice retained the ... More
Disease-causing mutations in the cystic fibrosis transmembrane conductance regulator determine the functional responses of alveolar macrophages.
'Alveolar macrophages (AMs) play a major role in host defense against microbial infections in the lung. To perform this function, these cells must ingest and destroy pathogens, generally in phagosomes, as well as secrete a number of products that signal other immune cells to respond. Recently, we demonstrated that murine ... More
Comparisons of CapG and gelsolin-null macrophages: demonstration of a unique role for CapG in receptor-mediated ruffling, phagocytosis, and vesicle rocketing.
AuthorsWitke W, Li W, Kwiatkowski DJ, Southwick FS
JournalJ Cell Biol
PubMed ID11514591
'Capping the barbed ends of actin filaments is a critical step for regulating actin-based motility in nonmuscle cells. The in vivo function of CapG, a calcium-sensitive barbed end capping protein and member of the gelsolin/villin family, has been assessed using a null Capg allele engineered into mice. Both CapG-null mice ... More
Herbimycin A inhibits both dephosphorylation and translocation of cofilin induced by opsonized zymosan in macrophagelike U937 cells.
'We previously reported that a 21-kDa phosphoprotein may play an important role in superoxide production through dephosphorylation by neutrophillike differentiated HL-60 cells (Suzuki et al., 1995, Biochim Biophys Acta 1266: 261-267). The phosphoprotein was identified as cofilin, an actin-binding protein, and the activation-induced changes in its intracellular distribution have been ... More
Differential involvement of Src family kinases in Fc gamma receptor-mediated phagocytosis.
AuthorsSuzuki T, Kono H, Hirose N, Okada M, Yamamoto T, Yamamoto K, Honda Z
JournalJ Immunol
PubMed ID10861086
'The tyrosine phosphorylation cascade originated from Fc gamma receptors (Fc gamma Rs) is essential for macrophage functions including phagocytosis. Although the initial step is ascribed to Src family tyrosine kinases, the role of individual kinases in phagocytosis signaling is still to be determined. In reconstitution experiments, we first showed that ... More
Inhibition of cytokinesis by a lipid metabolite, psychosine.
AuthorsKanazawa T, Nakamura S, Momoi M, Yamaji T, Takematsu H, Yano H, Sabe H, Yamamoto A, Kawasaki T, Kozutsumi Y
JournalJ Cell Biol
PubMed ID10811833
'Although a number of cellular components of cytokinesis have been identified, little is known about the detailed mechanisms underlying this process. Here, we report that the lipid metabolite psychosine (galactosylsphingosine), derived from galactosylceramide, induced formation of multinuclear cells from a variety of nonadherent and adherent cells due to inhibition of ... More
Studies on molecular regulation of phagocytosis in neutrophils. Con A-mediated ingestion and associated respiratory burst independent of phosphoinositide turnover, rise in [Ca2+]i, and arachidonic acid release.
AuthorsRossi F, Della Bianca V, Grzeskowiak M, Bazzoni F
JournalJ Immunol
PubMed ID2537359
'The role of the activation of phosphoinositide turnover and of the increase in cytosolic free calcium, [Ca2+]i, in the phagocytosis and associated activation of the respiratory burst was investigated. We report the results obtained on the phagocytosis of yeast cells mediated by Con A in normal and in Ca2+-depleted human ... More
Differences in the ingestion mechanisms of IgG and C3b particles in phagocytosis by neutrophils.
AuthorsHed J, Stendahl O
JournalImmunology
PubMed ID6279489
Methods for distinguishing ingested from adhering particles.
AuthorsHed J
JournalMethods Enzymol
PubMed ID3102897
Phagocytosis in Acanthamoeba: II. Soluble and insoluble mannose-rich ligands stimulate phosphoinositide metabolism.
AuthorsAllen PG, Dawidowicz EA
JournalJ Cell Physiol
PubMed ID2177061
The generation of second messengers during phagocytosis of yeast by Acanthamoeba castellanii was examined. The kinetics of binding and internalization of yeast by Acanthamoeba were measured and this was compared with the generation of known second messengers. We observed stimulated degradation of PI-4, 5-P2 to 1,4,5 IP3 with kinetics similar ... More
A new technique, requiring small amounts of cells, for the parallel study of chemiluminescence and phagocytosis via different receptors in the same cell population.
AuthorsSandgren CH, Nordling K, Björk I
JournalJ Immunol Methods
PubMed ID1907628
An assay permitting the parallel assessment of phagocytosis and chemiluminescence in the same cell population has been developed. The method is based on the phagocytosis of fluorescein isothiocyanate-conjugated yeast particles, either unopsonized or opsonized with complement factor C3 or IgG, by purified cells in suspension in a luminometer. Only a ... More
Flow cytometric quantitative determination of ingestion by phagocytes needs the distinguishing of overlapping populations of binding and ingesting cells.
AuthorsNuutila J, Lilius EM,
JournalCytometry A
PubMed ID15825183
BACKGROUND: The use of flow cytometry with fluorescently labeled particles provides the means to examine quantitatively the phagocytotic capacity of an individual phagocyte. This report describes an improved flow cytometric method of analysis for kinetic measurement of phagocytosis of fluorescein isothiocyanate (FITC)-labeled zymosan particles by human leukocytes. METHODS: FITC-labeled zymosan ... More
Flow cytometry distinction between adherent and phagocytized yeast particles.
AuthorsGiaimis J, Lombard Y, Poindron P, Muller CD
JournalCytometry
PubMed ID7835168
Our laboratory recently developed a light microscopy staining technique that provides a mean to distinguish between yeast that are simply bound to the surface of macrophages and yeast that have actually been phagocytized by macrophages (7). We adapted this technique by using fluorescent probes in order to test phagocytic activity ... More
A simple quantitative fluorimetric assay of in vitro phagocytosis in human neutrophils.
AuthorsOben JA, Foreman JC
JournalJ Immunol Methods
PubMed ID3136209
In general the in vitro assays of phagocytosis rely on microscope counting or radioisotopic detection of ingested particles or microbiological counting of non-ingested bacteria. A very simple, rapid, highly quantitative method using fluorescein-labelled bacteria was described by Vray et al. (Scand. J. Immunol. (1980) 11, 147) for non-human phagocytes. We ... More
Serum amyloid P component and C-reactive protein mediate phagocytosis through murine Fc gamma Rs.
AuthorsMold C, Gresham HD, Du Clos TW
JournalJ Immunol
PubMed ID11145702
The pentraxins, serum amyloid P component (SAP) and C-reactive protein (CRP) are acute-phase serum proteins in mice and humans, respectively. Although SAP binds to DNA and chromatin and affects clearance of these autoantigens, no specific receptor for SAP has been identified. CRP is an opsonin, and we have shown that ... More
Interaction of t-butylbicyclophosphorothionate with gamma-aminobutyric acid-gated chloride channels in cultured cerebral neurons.
AuthorsTehrani MH, Vaidyanathaswamy R, Verkade JG, Barnes EM
JournalJ Neurochem
PubMed ID2420933
The role of t-butylbicyclophosphorothionate (TBPS) as an antagonist of gamma-aminobutyric acid (GABA) was studied with primary cultures of neurons from the chick embryo cerebrum. The addition of GABA stimulated the uptake of 36Cl- by neurons and the dose dependence of this effect followed hyperbolic kinetics with a K0.5 = 1.3 ... More
Myeloperoxidase-dependent fluorescein chlorination by stimulated neutrophils.
AuthorsHurst JK, Albrich JM, Green TR, Rosen H, Klebanoff S
JournalJ Biol Chem
PubMed ID6325409
Hypochlorous acid (HOC1) rapidly chlorinates fluorescein compounds forming, sequentially, the corresponding 4'-chlorofluorescein and 4',5'-dichlorofluoresceins. Chlorination by cell-free myeloperoxidase-catalyzed chloride peroxidation systems gives rise to these compounds as well as variable amounts of isomeric compounds chlorinated in the 2'- and 2',7'-positions. The fluorescence intensity of the dianionic form of the dye ... More
Polyethylene glycol-modified GM-CSF expands CD11b(high)CD11c(high) but notCD11b(low)CD11c(high) murine dendritic cells in vivo: a comparative analysis with Flt3 ligand.
AuthorsDaro E, Pulendran B, Brasel K, Teepe M, Pettit D, Lynch DH, Vremec D, Robb L, Shortman K, McKenna HJ, Maliszewski CR, Maraskovsky E
JournalJ Immunol
PubMed ID10861034
Dendritic cells (DC) are potent APCs that can be characterized in the murine spleen as CD11b(high)CD11c(high) or CD11b(low)CD11c(high). Daily injection of mice of Flt3 ligand (FL) into mice transiently expands both subsets of DC in vivo, but the effect of administration of GM-CSF on the expansion of DC in vivo ... More
Studies on molecular regulation of phagocytosis and activation of the NADPH oxidase in neutrophils. IgG- and C3b-mediated ingestion and associated respiratory burst independent of phospholipid turnover and Ca2+ transients.
AuthorsDella Bianca V, Grzeskowiak M, Rossi F
JournalJ Immunol
PubMed ID2105997
The role of messengers derived from hydrolysis of phosphoinositides and other phospholipids, of the basal level of [Ca2+]i and of the increase in [Ca2+]i in phagocytosis and respiratory burst was investigated, using normal neutrophils and neutrophils Ca2(+)-depleted by pretreatment with Quin2/AM and EGTA. 1) Phagocytosis and respiratory burst in control ... More
Na+/H+ exchange activity during phagocytosis in human neutrophils: role of Fcgamma receptors and tyrosine kinases.
In neutrophils, binding and phagocytosis facilitate subsequent intracellular killing of microorganisms. Activity of Na+/H+ exchangers (NHEs) participates in these events, especially in regulation of intracellular pH (pHi) by compensating for the H+ load generated by the respiratory burst. Despite the importance of these functions, comparatively little is known regarding the ... More
Inhibition of the calcium-dependent tyrosine kinase (CADTK) blocks monocyte spreading and motility.
Freshly isolated peripheral blood monocytes lack focal adhesion kinase (p125(FAK)) but activate a second member of this kinase family, calcium-dependent tyrosine kinase (CADTK; also known as Pyk2/CAKbeta/RAFTK/FAK2), upon adhesion or stimulation with chemokines. To study the role of CADTK in monocyte adherence and motility, we performed immunocytochemical localization that showed ... More