Medio Neurobasal™ sin rojo fenol
Medio Neurobasal™ sin rojo fenol
Gibco™

Medio Neurobasal™ sin rojo fenol

El medio Neurobasal, sin rojo de fenol es un medio basal diseñado para el mantenimiento a largo plazo y laMás información
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Número de catálogoCantidad
12348017500 mL
Número de catálogo 12348017
Precio (USD)
165,00
Each
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Cantidad:
500 mL
Customize this product
Precio (USD)
165,00
Each
Añadir al carro de la compra
El medio Neurobasal, sin rojo de fenol es un medio basal diseñado para el mantenimiento a largo plazo y la maduración de neuronas prenatales y embrionarias sin necesidad de una capa de alimentación de astrocitos cuando se utiliza con suplementos Gibco B-27. Utilice esta formulación modificada del medio Neurobasal cuando la presencia de rojo de fenol interfiera en los resultados.

El medio Neurobasal es adecuado para su uso en la mayoría de las aplicaciones de células neuronales. Para otras aplicaciones, ofrecemos formulaciones alternativas de medios Neurobasal y suplementos B-27.

Para protocolos establecidos que usen medios Neurobasal, acceda a nuestro manual de protocolos de neurobiología Gibco.

Vea las publicaciones que utilizan productos de neurobiología Gibco en nuestra CellCite Gibco.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaCélulas neuronales
Línea de productosNeurobasal
Tipo de productoMedio neurobasal
Cantidad500 mL
Duración de almacenamiento12 meses a partir de la fecha de fabricación
Condiciones de envíoAmbiente
Probado paraEndotoxinas, rendimiento, esterilidad, osmolalidad, pH
ClasificaciónSin suero
Culture TypeCultivo de células neuronales
FormularioLíquido
Sin aditivosSin rojo fenol
Unit SizeEach
Contenido y almacenamiento
Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does the removal of phenol red from the Neurobasal Medium affect the culturing of neurons?

No, removal of phenol red from the Neurobasal Medium does not affect the culturing of neurons. Removal of phenol red allows culturing of neurons for studies involving certain receptors and downstream purification.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When should I use the Neurobasal Medium, minus phenol red?

We recommend using Neurobasal Medium, minus phenol red for studies involving certain receptors, downstream protein purification or other processes where the presence of phenol red is undesirable.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I understand that some media are worse than others for fluorescence imaging. How do I choose?

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Medio Neurobasal™ sin rojo fenol FAQs