TaqMan™ SNP Genotyping Assay, mouse
TaqMan™ SNP Genotyping Assay, mouse
Citas y referencias (1421)
Applied Biosystems™

TaqMan™ SNP Genotyping Assay, mouse

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Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNAMás información
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Número de catálogoCantidad
4351380L (2400 reactions), Made to Order
4351384S (300 reactions), Made to Order
4351382M (1000 reactions), Made to Order
Número de catálogo 4351380
Precio (USD)
-
Cantidad:
L (2400 reactions), Made to Order
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP), and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.

The predesigned TaqMan SNP Genotyping Mouse Assay collection consists of over 10,000 assays. For targets of interest that are not covered by the predesigned assay collection, Custom TaqMan SNP Genotyping Assays are available to meet your research needs.

Benefits:
• Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
• Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
• Guaranteed—if you are not satisfied with the performance of a TaqMan Assay, we’ll replace it at no cost, or credit your account*

Approximate ship time
4–6 days in North America and 6–10 days in Europe

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).

All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix (sold separately): TaqMan Genotyping Master Mix

*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.

For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Modificación del cebador 3'None
Modificación del cebador 5'None
DescripciónLa sonda de MGB VIC™ detecta el alelo 1; la sonda FAM-MGB detecta el alelo 2
Para utilizar con (equipo)7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler*

* If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR
system in order to detect and record fluorescent signals.
FormatoMade to Order
Características ecológicasEmbalaje sostenible
Modificación de sonda internaVIC™ (5'), FAM (5'), MGB (unión al surco menor) (3')
N.º de reacciones2400 reactions
Línea de productosTaqMan
Método de purificaciónExtracción en fase sólida (SPE)
Grado de pureza o calidadSin ARNasa, sin ADNasa
CantidadL (2400 reactions), Made to Order
Condiciones de envíoTemperatura ambiente
EspecieRatón
ObjetivoOver 10,000 mouse assays are available
Concentración80X
Para utilizar con (aplicación)Genotyping
FormularioFrozen
Etiqueta o tinteFAM, VIC
Unit SizeEach
Contenido y almacenamiento
1 tubo que contiene una mezcla de 40x (tamaños S y M) u 80x (tamaño L) de ensayo preformulado (2 sondas y 2 cebadores).

Almacenar a entre -15 y -25 °C.

Preguntas frecuentes

What is the maximum size of insertion/deletion that can be detected by a TaqMan SNP Genotyping Assay?

Assays can detect an insertion/deletion of up to 6 bases. If you desire an assay to detect a larger insertion or deletion you may want to consider our Custom Design Service (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/custom-services-reagents-real-time-pcr-qpcr/custom-services-real-time-pcr-assays.html).

What can I do if there are no predesigned assays to my SNP?

You can use our Custom Assay Design Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/snp-genotyping-taqman-assays/custom-snp-assays.html?ICID=ta-lm-custom%20taqman%20snp%20assay-Single%20Tube%20Custom%20TaqMan%C2%AE%20SNP%20Genotyping%20Assays) to submit your SNP sequence for an assay design. Alternatively, you can also use Primer Express Software to design an assay.

How do I find an assay to my SNP?

You can easily find predesigned TaqMan SNP Genotyping Assays with our user-friendly search tool at https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays/snp-genotyping-taqman-assays.html. Simply search by:

-Target (assay ID, rs number, gene symbol)
-Specific location on a chromosome

You can also watch this short video (https://www.youtube.com/watch?feature=player_embedded&v=qA4VWe8Jyrs) on how to search for assays.

Can the TaqMan SNP Genotyping Assays be used for allele quantification?

No. TaqMan SNP assays contain competitive probe sequences with a 1 bp mismatch where the SNP is located. The other probe will still bind at a lower efficiency, producing some signal for the other fluorophore. Therefore, these are not quantitative.

In my TaqMan SNP Genotyping Assay, which base is labeled with FAM or VIC dye?

The probe labels are provided in the assay documentation and also online. Simply look up the assay by typing the assay ID into the search bar on thermofisher.com. Click on the “View Details” button, and under the Product Details look for the Context Sequence. The assays are always in a [VIC/FAM dye] order. From the context sequence, you can see which base is first (thus labeled with VIC dye), and which is second (thus labeled with FAM dye).

View all TaqMan™ SNP Genotyping Assay, mouse, L (2400 reactions), Made to Order FAQs

Citations & References (1421)

Citations & References
Abstract
Determination, mechanism and monitoring of knockdown resistance in permethrin-resistant human head lice, Pediculus humanus capitis
Authors:Clark, JM
Journal:JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
PubMed ID:
Permethrin resistance has been reported worldwide and clinical failures to commercial pediculicides containing permethrin have likewise occurred. Permethrin resistance in head lice populations from the U.S. is widespread but is not yet uniform and the level of resistance is relatively low (∼4-8 fold). Permethrin-resistant lice are cross-resistant to pyrethrins, PBO-synergized ... More
Geographical mapping of a multifocal thyroid tumour using genetic alteration analysis & miRNA profiling
Authors:Aherne, ST; Smyth, PC; Flavin, RJ; Russell, SM; Denning, KM; Li, JH; Guenther, SM; O'Leary, JJ; Sheils, OM
Journal:Molecular Cancer
PubMed ID:
Background: Papillary thyroid carcinoma (PTC) frequently presents as multiple tumour-foci within a single thyroid gland or pluriform, with synchronous tumours comprising different histological variants, raising questions regarding its clonality. Among the genetic aberrations described in PTC, the BRAF V600E mutation and ret/PTC activation occur most commonly. Several studies have investigated ... More
Risk haplotype analysis for bovine paratuberculosis
Authors:Pinedo, PJ; Wang, CG; Li, Y; Rae, DO; Wu, RL
Journal:MAMMALIAN GENOME
PubMed ID:
Paratuberculosis (Johne's disease), caused by Mycobacterium avium subsp. paratuberculosis, is an important disease for bovines, although its genetic basis is poorly understood. In this study, three candidate genes were typed to study the associations between single nucleotide polymorphisms (SNPs) and paratuberculosis susceptibility (measured in a 1 or 0 form) at ... More
Risk haplotype analysis for bovine paratuberculosis
Authors:Pinedo, PJ; Wang, CG; Li, Y; Rae, DO; Wu, RL
Journal:JOURNAL OF NEURAL TRANSMISSION
PubMed ID:
Paratuberculosis (Johne's disease), caused by Mycobacterium avium subsp. paratuberculosis, is an important disease for bovines, although its genetic basis is poorly understood. In this study, three candidate genes were typed to study the associations between single nucleotide polymorphisms (SNPs) and paratuberculosis susceptibility (measured in a 1 or 0 form) at ... More
Association of Polymorphisms in Genes of the Homologous Recombination DNA Repair Pathway and Thyroid Cancer Risk
Authors:Bastos, HN; Antao, MR; Silva, SN; Azevedo, AP; Manita, I; Teixeira, V; Pina, JE; Gil, OM; Ferreira, TC; Limbert, E; Rueff, J; Gaspar, JF
Journal:THYROID
PubMed ID:
Background: Ionizing radiation exposure has been pointed out as a risk factor for thyroid cancer. The double-strand breaks induced by this carcinogen are usually repaired by homologous recombination repair pathway, a pathway that includes several polymorphic genes. Since there is a scarcity of data about the involvement of these gene ... More
View all TaqMan™ SNP Genotyping Assay, mouse, L (2400 reactions), Made to Order citations