Gránulos de recuento absoluto CountBright™ para citometría de flujo

Citas y referencias (7)

Invitrogen™

Gránulos de recuento absoluto CountBright™ para citometría de flujo

Los gránulos de recuento absoluto CountBright™ son una suspensión calibrada de microesferas con una fluorescencia brillante en una amplia gamaMás información

Número de catálogo	Cantidad
C36950	5 mL

Número de catálogo C36950

Precio (USD)

497,55

Añadir al carro de la compra

Cantidad:

Precio (USD)

497,55

Añadir al carro de la compra

Los gránulos de recuento absoluto CountBright™ son una suspensión calibrada de microesferas con una fluorescencia brillante en una amplia gama de longitudes de onda de excitación y emisión (UV a 635 nm de excitación y a 385-800 nm de emisión). Los gránulos de recuento absoluto CountBright™ se mezclan con la muestra celular y se prueban con citometría de flujo. Mediante la comparación de la relación entre eventos de los gránulos y eventos celulares es posible calcular el recuento absoluto de células. Los gránulos CountBright™ se mezclan en la muestra de prueba. Los recuentos celulares absolutos que utilizan este método de una sola plataforma son más precisos y menos complicados que la concentración celular determinada con pruebas de múltiples plataformas. Los gránulos de recuento absoluto CountBright™ se pueden utilizar con cualquier tipo de muestra, incluida sangre completa lisada/sin lavado.

Consulte la información sobre todos los gránulos de recuento de células de citometría de flujo.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Método de detecciónFluorescente

Diámetro (métrico)7 μm

Emisióncualquier láser (UV a 633/635)

Intervalo de longitud de onda de excitaciónUV a entre 635/385 y 800 nm

Para utilizar con (equipo)Citómetro de flujo

FormatoSolución

Nº de pruebas100

Cantidad5 mL

Condiciones de envíoTemperatura ambiente

Línea de productosCountBright

Tipo de productoGránulo de recuento absoluto

Unit SizeEach

Contenido y almacenamiento

Contiene 1 frasco de gránulos de recuento absoluto CountBright™ (5 ml).

Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

With the CountBright Plus Absolute Counting Beads/CountBright Absolute Counting Beads, instead of a tight cluster of beads on the scatter plot, I am seeing the beads cover a broad area. What is causing this?

Prior to taking an aliquot, make certain to thoroughly mix the beads. This may also be caused if the beads were ever frozen or the stock solution has any microbial contamination.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which buffers can I use with the CountBright Plus Absolute Counting Beads and CountBright Absolute Counting Beads?

You may use any physiological buffer or media in a pH range from pH 4 to 10. Avoid extremes of pH and high salt concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How are CountBright Plus Absolute Counting Beads different from CountBright Absolute Counting Beads?

Here are the differences:

CountBright Plus Absolute Counting Beads:
Bead Size: 4 µm
Excitation Range: 385 - 810 nm
Emission Range: 385 - 860 nm

CountBright Absolute Counting Beads:
Bead Size: 7 µm
Excitation Range: 350 - 635 nm
Emission Range: 350 - 810 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When using the Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry, some cells seem to have both red and green signal. Are these cells dead or alive or dying?

The green dye in the kit will label all the cells as it is a cell-permeant nucleic acid stain. The red dye is not cell permeant, and will only stain the cells with compromised membranes (dead cells). Therefore, any cells with red signal will be considered dead. It is possible that you will have some cells that are only red, some that are red and green, and some that are only green. Sometimes the red dye will displace the green dye. In any case, any red cells are dead.

Also, the green dye emission may bleed through into the red channel. Do a single-color staining and examine under both green and red filter sets to determine the level of bleedthrough. To avoid this bleedthrough, use a lower concentration of dye, and, if possible, use narrow bandpass filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How can I count my bacteria by flow cytometry?

There are several options. We have two fluorescence based kits that are useful for bacterial counting: Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856) and Bacteria Counting kit, for flow cytometry (Cat. No. B7277). Another option is the Flow Cytometry Sub-micron Particle Size Reference Kit (Cat. No. F13839).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Gránulos de recuento absoluto CountBright™ para citometría de flujo FAQs

Citations & References (7)

Citations & References

Abstract

An impaired transendothelial migration potential of chronic lymphocytic leukemia (CLL) cells can be linked to ephrin-A4 expression.

Authors:Trinidad EM, Ballesteros M, Zuloaga J, Zapata A, Alonso-Colmenar LM,

Journal:Blood

PubMed ID:19828693

'Chronic lymphocytic leukemia (CLL) cell migration into lymphoid tissues is an important aspect of the pathobiology of this disease. Here, we investigated the role of ephrin-A4 (EFNA4) in the transendothelial migration (TEM) capacity of CLL and normal B cells through interacting with endothelial EphA2 (erythropoietin-producing hepatocellular carcinoma). CLL cells showed ... More

Analysis of microRNA and protein transfer by exosomes during an immune synapse.

Authors:Villarroya-Beltri C, Gutiérrez-Vázquez C, Sánchez-Madrid F, Mittelbrunn M,

Journal:

PubMed ID:23719941

'Immune cells release microRNA-containing exosomes that can be taken up by recipient cells. Exosomes can thus act as mediators of cell-cell communication through direct exchange of genetic material between cells. Exosome-mediated transfer of miRNAs between T cells and antigen-presenting cells (APCs) can take place over long distances. Our work has ... More

Inhibition of NF-?B-mediated signaling by the cyclin-dependent kinase inhibitor CR8 overcomes prosurvival stimuli to induce apoptosis in chronic lymphocytic leukemia cells.

Authors:Cosimo E, McCaig AM, Carter-Brzezinski LJ, Wheadon H, Leach MT, Le Ster K, Berthou C, Durieu E, Oumata N, Galons H, Meijer L, Michie AM,

Journal:Clin Cancer Res

PubMed ID:23532892

Chronic lymphocytic leukemia (CLL) is currently incurable with standard chemotherapeutic agents, highlighting the need for novel therapies. Overcoming proliferative and cytoprotective signals generated within the microenvironment of lymphoid organs is essential for limiting CLL progression and ultimately developing a cure. We assessed the potency of cyclin-dependent kinase (CDK) inhibitor CR8, ... More

PKCa negatively regulates in vitro proplatelet formation and in vivo platelet production in mice.

Authors:Williams CM, Harper MT, Poole AW,

Journal:Platelets

PubMed ID:23402219

Proplatelet formation is a part of the intricate process by which platelets are generated by their precursor cell, the megakaryocyte. The processes that drive megakaryocyte maturation and platelet production are however still not well understood. The protein kinase C (PKC) family of serine/threonine kinases has been demonstrated as an important ... More

CD26 inhibition enhances perfusion recovery in ApoE-/-mice.

Authors:Haverslag RT, de Groot D, Grundmann S, Meder B, Goumans MJ, Pasterkamp G, Hoefer IE, de Kleijn DP,

Journal:Curr Vasc Pharmacol

PubMed ID:23391419

The adaptive growth of blood vessels is important to prevent tissue loss following arterial occlusion. Extravasation of monocytes is essential for this process. The peptidase CD26 targets SDF-1 alpha, a chemokine regulating monocyte trafficking. We hypothesized that blocking SDF-1 alpha inactivation, using a commercially available CD26 inhibitor, accelerates perfusion recovery ... More

View all Gránulos de recuento absoluto CountBright™ para citometría de flujo citations