Tinción de mielina fluorescente FluoroMyelin™ verde: solución en agua

Citas y referencias (15)

Invitrogen™

Tinción de mielina fluorescente FluoroMyelin™ verde: solución en agua

La tinción fluorescente de mielina FluoroMyelin™ Green permite un etiquetado rápido y selectivo de la mielina en cortes criogénicos cerebralesMás información

Número de catálogo	Cantidad
F34651	1 mL

Número de catálogo F34651

Precio (USD)

558,90

Añadir al carro de la compra

Cantidad:

Precio (USD)

558,90

Añadir al carro de la compra

La tinción fluorescente de mielina FluoroMyelin™ Green permite un etiquetado rápido y selectivo de la mielina en cortes criogénicos cerebrales en un solo paso de etiquetado de 20 minutos más lavados. Esta tinción se puede utilizar junto con anticuerpos y otros colorantes, y con métodos histoquímicos estándar para el material de cortes criogénicos.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorVerde

Método de detecciónFluorescencia

Para utilizar con (equipo)Microscopio de fluorescencia

Etiqueta o tinteFluoroMyelin™ Green

Tipo de productoTinción de mielina verde fluorescente

Cantidad1 mL

Condiciones de envíoTemperatura ambiente

Localización subcelularMielina

Excitation/Emission479/598 nm

Línea de productosFluoroMyelin

Unit SizeEach

Contenido y almacenamiento

Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I used one of your FluoroMyelin stains and noticed it stains cells other than glial cells. Is there something wrong with the product?

FluoroMyelin is a lipid stain, any lipid can be stained by it but there is a higher lipid content in myelin that it will stain much more intensely than other membranes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the FluoroMyelin stains be used on neurons in culture?

We have only tested them on mouse brain cryosections. They might work on neurons in culture, but would likely stain the membrane and there would be some endocytosis of the stains. Myelin sheaths may show enhanced intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the FluoroMyelin myelin stains be used on paraffin sections?

We have only tested them on mouse brain cryosections.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the FluoroMyelin myelin stains?

They are proprietary lipid stains that stain myelin, but are not neuron specific.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (15)

Citations & References

Abstract

Akt1 deficiency affects neuronal morphology and predisposes to abnormalities in prefrontal cortex functioning.

Authors:Lai WS, Xu B, Westphal KG, Paterlini M, Olivier B, Pavlidis P, Karayiorgou M, Gogos JA

Journal:Proc Natl Acad Sci U S A

PubMed ID:17077150

'There is accumulating evidence that AKT signaling plays a role in the pathogenesis of schizophrenia. We asked whether Akt1 deficiency in mice results in structural and functional abnormalities in prefrontal cortex (PFC). Exploratory transcriptional profiling revealed concerted alterations in the expression of PFC genes controlling synaptic function, neuronal development, myelination, ... More

Inhibitor of apoptosis protein (IAP) profiling in experimental autoimmune encephalomyelitis (EAE) implicates increased XIAP in T lymphocytes.

Authors:Moore CS, Hebb AL, Robertson GS,

Journal:J Neuroimmunol

PubMed ID:18055022

'In multiple sclerosis (MS) and its widely accepted animal model, experimental autoimmune encephalomyelitis (EAE), the failure of autoreactive immune cells to undergo apoptosis is thought to contribute to CNS tissue damage and disease progression. Promoting apoptosis of myelin-reactive immune cells in diseases such as MS, may delay disease progression and ... More

Prion protein (PrPc) positively regulates neural precursor proliferation during developmental and adult mammalian neurogenesis.

Authors:Steele AD, Emsley JG, Ozdinler PH, Lindquist S, Macklis JD

Journal:Proc Natl Acad Sci U S A

PubMed ID:16492732

'The misfolding of the prion protein (PrP(c)) is a central event in prion diseases, yet the normal function of PrP(c) remains unknown. PrP(c) has putative roles in many cellular processes including signaling, survival, adhesion, and differentiation. Given the abundance of PrP(c) in the developing and mature mammalian CNS, we investigated ... More

Neuronal glutathione deficiency and age-dependent neurodegeneration in the EAAC1 deficient mouse.

Authors:Aoyama K, Suh SW, Hamby AM, Liu J, Chan WY, Chen Y, Swanson RA

Journal:Nat Neurosci

PubMed ID:16311588

Uptake of the neurotransmitter glutamate is effected primarily by transporters expressed on astrocytes, and downregulation of these transporters leads to seizures and neuronal death. Neurons also express a glutamate transporter, termed excitatory amino acid carrier-1 (EAAC1), but the physiological function of this transporter remains uncertain. Here we report that genetically ... More

Effect of chronic continuous or intermittent hypoxia and reoxygenation on cerebral capillary density and myelination.

Authors:Kanaan A, Farahani R, Douglas RM, Lamanna JC, Haddad GG

Journal:Am J Physiol Regul Integr Comp Physiol

PubMed ID:16322350

Chronic hypoxia, whether continuous (CCH) or intermittent (CIH), occurs in many neonatal pathological conditions, such as bronchopulmonary dysplasia and obstructive sleep apnea. In this study, we explored the effect of CCH and CIH on cerebral capillary density and myelination. We subjected CD-1 mice starting at postnatal day 2 to either ... More

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