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Invitrogen™
HPF (Hydroxyl Radical and Peroxynitrite Sensor)
La aminofenil fluoresceína (APF, A36003) y la hidroxifenil fluoresceína (HPF), dos indicadores de especies reactivas de oxígeno (ROS) desarrollados porMás información
| Número de catálogo | Cantidad |
|---|---|
| H36004 | 470 μL |
Número de catálogo H36004
Precio (USD)
-
Cantidad:
470 μL
La aminofenil fluoresceína (APF, A36003) y la hidroxifenil fluoresceína (HPF), dos indicadores de especies reactivas de oxígeno (ROS) desarrollados por T. Nagano, ofrecen mayor especificidad y estabilidad que el diacetato de diclorodihidrofluoresceína (H2DCFDA, D399). La APF y la HPF muestran una reactividad no selectiva limitada y una resistencia relativamente alta a la oxidación inducida por la luz. Estos dos nuevos derivados de la fluoresceína no son fluorescentes hasta que reaccionan con el radical hidroxilo o el anión peroxinitrito; la APF también reacciona con el anión hipoclorito. Juntos, estos dos indicadores ROS pueden detectar selectivamente el anión hipoclorito. Tras la oxidación, tanto la APF como la HPF presentan fluorescencia verde brillante (excitación/emisión máximas ∼490/515 nm), de modo que son compatibles con cualquier instrumento que pueda detectar fluoresceína.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
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Cantidad470 μL
Tipo de productoROS Indicator
Unit SizeEach
Citations & References (30)
Citations & References
Abstract
Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?
Journal:Br J Pharmacol
PubMed ID:15155533
'Free radicals and other reactive species (RS) are thought to play an important role in many human diseases. Establishing their precise role requires the ability to measure them and the oxidative damage that they cause. This article first reviews what is meant by the terms free radical, RS, antioxidant, oxidative
Externally applied pressure activates pancreatic stellate cells through the generation of intracellular reactive oxygen species.
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:17761838
'Local tissue pressure is higher in chronic pancreatitis than in the normal pancreas. We reported recently that pressure application induces synthesis of extracellular matrix (ECM) and cytokines in pancreatic stellate cells (PSCs) and that epigallocatechin gallate (EGCG), a potent antioxidant, inhibits the transformation of PSCs from quiescent to activated phenotype
The detection and quantification of highly reactive oxygen species using the novel HPF fluorescence probe in a rat model of focal cerebral ischemia.
Journal:Neurosci Res
PubMed ID:16168507
'A novel fluorescence probe, 2-[6-(4''-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoic acid (HPF) was used to investigate the generation of highly reactive oxygen species (hROS) under ischemia both in vitro and in vivo. In the in vitro study, HT 22 cells were used to demonstrate that was predominantly detected in the cytoplasm, which coincides
Time to fatigue is increased in mouse muscle at 37 degrees C; the role of iron and reactive oxygen species.
Journal:J Physiol
PubMed ID:19470779
'Studies exploring the rate of fatigue in isolated muscle at 37 degrees C have produced mixed results. In the present study, muscle fibre bundles from the mouse foot were used to study the effect of temperature on the rate of muscle fatigue. Provided iron was excluded from the solutions, time
Fluorescence probes used for detection of reactive oxygen species.
Journal:J Biochem Biophys Methods
PubMed ID:16297980
'Endogenously produced pro-oxidant reactive species are essential to life, being involved in several biological functions. However, when overproduced (e.g. due to exogenous stimulation), or when the levels of antioxidants become severely depleted, these reactive species become highly harmful, causing oxidative stress through the oxidation of biomolecules, leading to cellular damage