Nigericin, Free Acid
Nigericin, Free Acid
Citas y referencias (282)
Invitrogen™

Nigericin, Free Acid

La calibración intracelular de la respuesta de fluorescencia de los indicadores de pH citosólicos se realiza típicamente utilizando el K+/H+Más información
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Número de catálogoCantidad
N149510 mg
Número de catálogo N1495
Precio (USD)
489,70
Each
Añadir al carro de la compra
Cantidad:
10 mg
Precio (USD)
489,70
Each
Añadir al carro de la compra
La calibración intracelular de la respuesta de fluorescencia de los indicadores de pH citosólicos se realiza típicamente utilizando el K+/H+ ionóforo nigericina, que causa el equilibrio del pH intracelular y extracelular en presencia de una concentración despolarizante de K+ extracelular.

Obtenga más información sobre los indicadores de iones, incluidos los indicadores de calcio, potasio, pH y potencial de la membrana ›

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
IndicadorIonóforo
Fórmula molecularC40H68O11
Cantidad10 mg
Almacenamiento recomendadoAlmacenar en congelador (de -5 °C a -30 °C)
Condiciones de envíoTemperatura ambiente
Forma físicaSólido
Tipo de productoNigericina
Unit SizeEach

Citations & References (282)

Citations & References
Abstract
Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.
Authors:Martínez-Zaguilán R,Gurulé MW,Lynch RM
Journal:The American journal of physiology
PubMed ID:8967445
Measurement of intracellular pH.
Authors:Boyer MJ, Hedley DW
Journal:Methods Cell Biol
PubMed ID:7532259
CFTR regulates phagosome acidification in macrophages and alters bactericidal activity.
Authors:Di A, Brown ME, Deriy LV, Li C, Szeto FL, Chen Y, Huang P, Tong J, Naren AP, Bindokas V, Palfrey HC, Nelson DJ
Journal:Nat Cell Biol
PubMed ID:16921366
'Acidification of phagosomes has been proposed to have a key role in the microbicidal function of phagocytes. Here, we show that in alveolar macrophages the cystic fibrosis transmembrane conductance regulator Cl- channel (CFTR) participates in phagosomal pH control and has bacterial killing capacity. Alveolar macrophages from Cftr-/- mice retained the ... More
Rapid flow cytometric method for measuring senescence associated beta-galactosidase activity in human fibroblasts.
Authors:Noppe G, Dekker P, de Koning-Treurniet C, Blom J, van Heemst D, Dirks RW, Tanke HJ, Westendorp RG, Maier AB,
Journal:Cytometry A
PubMed ID:19777541
'Senescence associated-beta-galactosidase (SA-beta-gal) activity is a widely used marker for cellular senenescence. SA-beta-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to ... More
Effects of cytoplasmic acidification on clathrin lattice morphology.
Authors:Heuser J
Journal:J Cell Biol
PubMed ID:2563729
'Reducing the internal pH of cultured cells by several different protocols that block endocytosis is found to alter the structure of clathrin lattices on the inside of the plasma membrane. Lattices curve inward until they become almost spherical yet remain stubbornly attached to the membrane. Also, the lattices bloom empty ... More
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