NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)
NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)
Citas y referencias (1)
Invitrogen™

NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels)

El kit de tampones de SDS MES NuPAGE se ha diseñado para la separación de proteínas de tamaño pequeño aMás información
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Número de catálogoCantidad
NP00601 kit
Número de catálogo NP0060
Precio (USD)
-
Cantidad:
1 kit
El kit de tampones de SDS MES NuPAGE se ha diseñado para la separación de proteínas de tamaño pequeño a mediano en geles Bis-Tris NuPAGE e incluye los siguientes tampones:
• Tampón de desplazamiento de SDS NuPAGE MES (20X, 500 ml, n.º de cat. cat. NP0002)
• Agente reductor de muestras NuPAGE (10X, 250 µl, n.º de cat. NP0004)
• Antioxidante NuPAGE (n.º de cat. NP0005)
• Tampón de muestra de LDS NuPAGE (4X, 10 ml, n.º de cat. NP0007)

Compare los patrones de migración de proteínas mediante MES y MOP en geles de Bis-Tris NuPAGE

Descubra todos los tampones y reactivos disponibles para SDS-PAGE

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de gelGel SDS PAGE
Cantidad1 kit
Gel CompatibilityGel de tricina Novex™
Línea de productosNuPAGE
Tipo de productoKit del tampón
Unit SizeEach
Contenido y almacenamiento
Contiene
• Tampón de desplazamiento de SDS MES NuPAGE™ (20X, 500 ml)
• Agente reductor de muestras NuPAGE™ (10X, 250 µl), que contiene 500 mM de ditiotreitol
• Antioxidante NuPAGE™ (15 ml)
• Tampón de muestras de LDS NuPAGE™ (10 ml)
Almacenar cada uno a 4 °C. Caliente el tampón de muestra de LDS NuPAGE™ a 25 °C antes de usarlo.

Preguntas frecuentes

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can beta-mercaptoethanol (BME) be used rather than DTT as the reducing agent in the NuPAGE LDS Sample Buffer?

Either BME or DTT can be used in the NuPAGE LDS Sample Buffer.

Make sure that a fresh solution of BME is used. FINAL concentration:

DTT 50-100 mM

BME 2-5%

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) FAQs

Citations & References (1)

Citations & References
Abstract
Spermidine but not spermine is essential for hypusine biosynthesis and growth in Saccharomyces cerevisiae: Spermine is converted to spermidine in vivo by the FMS1-amine oxidase.
Authors:Chattopadhyay MK, Tabor CW, Tabor H,
Journal:Proc Natl Acad Sci U S A
PubMed ID:14617780
In our earlier work we showed that either spermidine or spermine could support the growth of spe2Delta or spe3Delta polyamine-requiring mutants, but it was unclear whether the cells had a specific requirement for either of these amines. In the current work, we demonstrate that spermidine is specifically required for the ... More