The Tango™ CXCR7-bla U2OS cells contain the human Chemokine (C-X-C motif) receptor 7 (CXCR7) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element. The Tango™ CXCR7-bla U2OS cells are functionally validated for Z' and EC50 concentrations of SDF1a. In addition, Tango™ CXCR7-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_020311.2
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Dividing Cells
Cytoplasmic Tail
Native Tail
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
CXCR7
Human Protein Class
G-Protein Coupled Receptor (GPCR)
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™, Tango™
Readout
End Point
Target Entry
CXCR7
Type
U2OS Cell
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCR
Contents & Storage
Contents: K1832 Tango™ CXCR7-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ CXCR7-bla U2OS cells • Tango™ CXCR7-bla U2OS Assay Protocol • Certificate of Analysis
Storage: The Tango™ CXCR7-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.