Invitrogen™

Platinum™ Hot Start PCR Master Mix (2X)

Número do catálogo: 13000013
Invitrogen™

Platinum™ Hot Start PCR Master Mix (2X)

Número do catálogo: 13000013
Número do catálogo
13000013
Tamanho da unidade
200 reactions
Preço (BRL)
Preço 2.245,80
Seu Preço
Disponibilidade
***
Quantidade
Número do catálogo
Tamanho da unidade
50 reactions
Preço (BRL)
Preço 598,50
Seu Preço
Disponibilidade
***
Quantidade
Número do catálogo
Tamanho da unidade
1000 reactions
Preço (BRL)
Preço 8.823,60
Seu Preço
Disponibilidade
***
Quantidade
Número do catálogoTamanho da unidadePreço (BRL)DisponibilidadeQuantidade
13000013200 reactions
Preço 2.245,80
Seu Preço
***
1300001250 reactions
Preço 598,50
Seu Preço
***
130000141000 reactions
Preço 8.823,60
Seu Preço
***
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Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup. It retains all the features of Platinum Taq DNA Polymerase: specificity, robustness and reliability.

Product features:
• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room temperature reaction setup
• Versatile formulation for broad range of amplicons

About Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The antibodies dissociate during the initial PCR denaturation step and DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity.

Using Platinum Hot Start PCR Master Mix
Convenient Platinum Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. It is supplied with a separate vial of Platinum GC Enhancer, formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing.

Note: For superior PCR performance, we recommend the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology. Platinum II Hot-Start PCR Master Mix (2X) contains Platinum II Taq Hot-Start DNA Polymerase in a ready-to-use mixture with Platinum II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup.

Especificações

Format
Tube
GC-Rich PCR Performance
Medium
Polymerase
Platinum Taq DNA Polymerase
Reaction Speed
Standard
Label or Dye
Unlabeled
Product Line
Platinum™
Product Type
Hot Start PCR Master Mix
Shipping Condition
Dry Ice
Sufficient For
200 Reactions
Concentration
2X
Fidelity (vs. Taq)
1 X
Hot Start
Built-In Hot Start
No. of Reactions
200 reactions
Overhang
3'-A
Reaction Format
SuperMix or Master Mix
Size (Final Product)
5 kb or less
Starting Material
DNA

Conteúdo e armazenamento

Each box contains:
• 4 x 1.25 mL Platinum Hot Start PCR 2X Master Mix
• 2 x 1.25 mL Platinum GC Enhancer
• 4 x 1.25 mL water, nuclease-free
Store at -20°C in a non-frost-free freezer.

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