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Bilirubin Oxidase Polyclonal Antibody, Biotin
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FIGURE: 1 / 1
Bilirubin Oxidase Antibody (200-106-212S) in WB
Western Blot of Goat anti-Bilirubin Oxidase (Myrothecium verrucaria) Antibody Biotin Conjugated. Lane 1: Bilirubin Oxidase (Myrothecium verrucaria). Load: 50 ng per lane. Primary antibody: Goat anti-Bilirubin Oxidase (Myrothecium verrucaria) Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP Streptavidin (p/n S000-03) 1:40,000 in MB-070 for 30 min at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 64 kDa, 60 kDa for Bilirubin Oxidase. Other band(s): Bilirubin Oxidase splice variants and isof... View More
Product Details
200-106-212S
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fungi
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Bilirubin Oxidase [Myrothecium verrucaria]
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
25 µg
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Bilirubin Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria]. Cross reactivity against Bilirubin Oxidase from other sources is unknown.
Target Information
Bilirubin Oxidase oxidizes bilirubin and other tetrapyrroles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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