Pierce™ DTT (Dithiothreitol)

Dithiothreitol is a popular reducing agent for many protein research applications, including reducing polyacrylamide electrophoresis (SDS-PAGE).Related ProductsBond-Breaker™ TCEP Solution, NeutralLeia mais

Número do catálogo	Quantity
20290	5 g

Número do catálogo 20290

Preço (BRL)

Quantity:

Request bulk or custom format

Dithiothreitol is a popular reducing agent for many protein research applications, including reducing polyacrylamide electrophoresis (SDS-PAGE).

Related Products
Bond-Breaker™ TCEP Solution, Neutral pH

For Research Use Only. Not for use in diagnostic procedures.

Especificações

DescriptionPierce DTT, Cleland's Reagent

FormPowder

Product LinePierce

Quantity5 g

Product TypeDithiothreitol

Unit SizeEach

Conteúdo e armazenamento

Store at 4°C.

Frequently asked questions (FAQs)

How do I prepare a 1 M DTT solution from powder and how should I store the solution?

Preparing 1 M DTT:
1) Dissolve 3.09 g DTT in 20 mL distilled H2O.
2) Dispense into 1 mL aliquots.
3) Store the aliquots at -20 degrees C.
Note: Upon thawing the vial, there may be some precipitation that can be re-dissolved by warming and mixing.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can you please recommend a method for reduction, alkylation, and digestion of an antibody?

Prepare 1 mg/mL antibody in 7.0 M Guanidine HCl, 100 mM Tris, pH 8.3. For reduction, add DTT (Cat. Nos. A39255, 20290) solution at 10 mM final concentration and incubate for 30 mins at room temperature. For alkylation, add sodium iodoacetate at 20 mM final concentration and incubate in the dark for 20 mins at room temperature. Perform buffer exchange followed by digestion using Pierce MS grade Trypsin, Cat. Nos. 90058, 90057 (1:10 enzyme to substrate ratio).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.