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Citações e referências (87)
Invitrogen™
Biotin-X, SE (6-((Biotinoyl)Amino)Hexanoic Acid, Succinimidyl Ester (Biotinamidocaproate, N-Hydroxysuccinimidyl Ester))
The amine-reactive biotin-X, SE can be used to attach this important hapten to biomolecules of interest for subsequent detection withLeia mais
| Número do catálogo | Quantity |
|---|---|
| B1582 conhecido também como B-1582 | 100 mg |
Número do catálogo B1582
conhecido também como B-1582
Preço (BRL)
-
Quantity:
100 mg
The amine-reactive biotin-X, SE can be used to attach this important hapten to biomolecules of interest for subsequent detection with streptavidin, avidin or NeutrAvidin™ biotin-binding protein.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Chemical ReactivityAmine
Label or DyeBiotin-X
Product TypeBiotin-XX SE
Quantity100 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
Label TypeBiotin and Other Haptens
Unit SizeEach
Conteúdo e armazenamento
Store in freezer (-5 to -30°C).
Citações e referências (87)
Citações e referências
Abstract
Peptide biotinylation with amine-reactive esters: differential side chain reactivity.
Journal:Peptides
PubMed ID:9437720
N-hydroxysuccinimide (NHS) esters of biotin are reported to react specifically with amino groups of peptides and proteins. However, we have found that these reagents can readily acylate other functional groups in specific peptide sequences under relatively mild conditions. We have extended our inquiry of sequence-dependent acylation by evaluating the reactivity
Enumeration of antigen-specific IgE responses at the single-cell level by an ELISA plaque assay.
Journal:J Immunol Methods
PubMed ID:1703186
In vitro elicitation and enumeration of antigen-specific IgE-secreting cells in antigen-primed murine lymphocyte cultures can be reproducibly and accurately quantitated. Rat anti-mouse IgE monoclonal antibodies (Mab anti-epsilon) were developed. Antigen-specific IgE-secreting cells can be detected on antigen or MAb anti-epsilon coated nitrocellulose discs pasted to 24-well culture plates. This IgE
A novel fluorescent toxin to detect and investigate Kv1.3 channel up-regulation in chronically activated T lymphocytes.
Journal:J Biol Chem
PubMed ID:12511563
T lymphocytes with unusually high expression of the voltage-gated Kv1.3 channel (Kv1.3(high) cells) have been implicated in the pathogenesis of experimental autoimmune encephalomyelitis, an animal model for multiple sclerosis. We have developed a fluoresceinated analog of ShK (ShK-F6CA), the most potent known inhibitor of Kv1.3, for detection of Kv1.3(high) cells
Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques.
Journal:J Cell Biol
PubMed ID:1659577
'We previously demonstrated that the gap junction protein connexin43 is translated as a 42-kD protein (connexin43-NP) that is efficiently phosphorylated to a 46,000-Mr species (connexin43-P2) in gap junctional communication-competent, but not in communication-deficient, cells. In this study, we used a combination of metabolic radiolabeling and immunoprecipitation to investigate the assembly
Localized suppression of RhoA activity by Tyr31/118-phosphorylated paxillin in cell adhesion and migration.
Journal:J Cell Biol
PubMed ID:12446743
'RhoA activity is transiently inhibited at the initial phase of integrin engagement, when Cdc42- and/or Rac1-mediated membrane spreading and ruffling predominantly occur. Paxillin, an integrin-assembly protein, has four major tyrosine phosphorylation sites, and the phosphorylation of Tyr31 and Tyr118 correlates with cell adhesion and migration. We found that mutation of