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The Tango™ OPRM1-bla U2OS cells contain the human Opioid Receptor Mu 1 (OPRM1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ OPRM1-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of DAMGO. In addition, Tango™ OPRM1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
The Tango™ OPRM1-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of DAMGO. In addition, Tango™ OPRM1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
Especificações
Accession Number
NM_000914
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Dividing Cells
Cytoplasmic Tail
Tail from AVPR2 GPCR
Detection Method
Fluorescent
Gene Symbol
OPRM1
Population Status
Single Cell Clone Origin
Product Line
Tango™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
OPRM1
Type
U2OS Cell
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCR
Conteúdo e armazenamento
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
o Tango™ OPRM1-bla U2OS cells
o Tango™ OPRM1-bla U2OS Assay Protocol
o Certificate of Analysis
The Tango™ OPRM1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Includes:
o Tango™ OPRM1-bla U2OS cells
o Tango™ OPRM1-bla U2OS Assay Protocol
o Certificate of Analysis
The Tango™ OPRM1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
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