The Tango™ GPR109A-bla U2OS cells contain the human Nicotinic Acid Receptor (GPR109A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ GPR109A-bla U2OS cells are functionally validated for Z' and EC50 concentrations of Nicotinic Acid. In addition, Tango™ GPR109A-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
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Especificações
Accession Number
NM_177551
Assay Entry
Cell-based beta-arrestin recruitment
Cell State
Dividing Cells
Cytoplasmic Tail
Native Tail
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
GPR109A
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™, Tango™
Readout
End Point
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
GPR109A
Type
U2OS Cell
Cell Line
U2OS
System Type
Tango™
Druggable Target
GPCR
Conteúdo e armazenamento
Contents: K1791 Tango™ GPR109A-bla U2OS cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • Tango™ GPR109A-bla U2OS cells • Tango™ GPR109A-bla U2OS Assay Protocol • Certificate of Analysis
Storage: The Tango™ GPR109A-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Figuras
Documentos e downloads
Certificados
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