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ThPOK Monoclonal Antibody (2POK), PE, eBioscience™
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FIGURE: 1 / 1
ThPOK Antibody (12-5928-80) in Flow
C57Bl/6 thymocytes were stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and Anti-Mouse CD8a FITC (Product # 11-0081-82), followed by intracellular staining with 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.125 µg of Anti-Mouse ThPOK PE using the Foxp3 Staining Buffer Set and protocol (Product # 00-5523-00). The histogram demonstrates ThPOK staining of CD4 SP (purple histogram) and CD8 SP (orange histogram) thymocytes.
Product Details
12-5928-80
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
2POK
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Description: This 2POK monoclonal antibody reacts with mouse ThPOK, which is also known as c-Krox and Zbtb7b. In hematopoietic cells, this 78 kDa transcription factor is expressed in thymocytes and T lymphocytes in the spleen and lymph nodes. In non-hematopoietic cells, ThPOK can be detected in the skin, heart, smooth muscle, and liver. Studies demonstrate that ThPOK promotes CD4+ lineage differentiation by inhibiting the expression of CD8 lineage-specific genes such as Runx3. ThPOK has also been shown to inhibit Cd4 silencing, another key facet required for differentiation to the CD4+ lineage. In addition to its role in thymocyte development, ThPOK is involved in the effector functions of invariant natural killer T (iNKT) cells and gamma delta T cells.
Applications Reported: This 2POK antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 2POK antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ZFP67 is an early growth response gene that encodes a zinc finger-containing transcription factor that binds to the promoter regions of type I collagen genes (e.g., COL1A1; MIM 120150) and has a role in development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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