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Citations & References (8)
Thermo Scientific™
EZ-Link™ NHS-PEG4 Biotinylation Kit
The Thermo Scientific EZ-Link NHS-PEG4 Biotinylation Kit contains EZ-Link NHS-PEG4-Biotin and accessory reagents required for 8 1–10 mg protein labelingRead more
| Catalog Number | Quantity |
|---|---|
| 21455 | 8 Reaction Kit |
Catalog number 21455
Price (CLP)
443.290
Each
Quantity:
8 Reaction Kit
Price (CLP)
443.290
Each
The Thermo Scientific EZ-Link NHS-PEG4 Biotinylation Kit contains EZ-Link NHS-PEG4-Biotin and accessory reagents required for 8 1–10 mg protein labeling reactions. EZ-Link NHS-PEG4-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
Features of EZ-Link NHS-PEG4-Biotin:
• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
• Pegylated – spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
• Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Long reach – spacer arm (total length added to target) is 29 angstroms; this reduces steric hindrance when binding to avidin molecules
NHS-PEG4-Biotin is a long (29.0Å), pegylated, water-soluble, NHS-ester biotinylation reagent to label amines and maximize solubility of antibodies and other proteins. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance for binding to avidin molecules.
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.
Varieties of biotin NHS-ester reagents differ in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF. Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable. Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.
Related Products
EZ-Link™ NHS-PEG4-Biotin
EZ-Link™ Micro NHS-PEG4-Biotinylation Kit
Features of EZ-Link NHS-PEG4-Biotin:
• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
• Pegylated – spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
• Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Long reach – spacer arm (total length added to target) is 29 angstroms; this reduces steric hindrance when binding to avidin molecules
NHS-PEG4-Biotin is a long (29.0Å), pegylated, water-soluble, NHS-ester biotinylation reagent to label amines and maximize solubility of antibodies and other proteins. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance for binding to avidin molecules.
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.
Varieties of biotin NHS-ester reagents differ in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF. Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable. Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.
Related Products
EZ-Link™ NHS-PEG4-Biotin
EZ-Link™ Micro NHS-PEG4-Biotinylation Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionEZ-Link NHS-PEG4 Biotinylation Kit
Labeling Scale1 to 10 mg
Product LineEZ-Link
Product TypeNHS-PEG4 Biotinylation Kit
Quantity8 Reaction Kit
Labeling TargetAntibodies, Proteins
Label or DyeBiotin
SolubilityWater
SpacerPegylated
Unit SizeEach
Contents & Storage
Sufficient For: 8 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link NHS-PEG4-Biotin, 8 x 2 mg
• PBS Pack (makes 500mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10mg
Store avidin and microtubes of biotin reagent at -20°C. Store PBS at room temperature. Store remaining kit components at 4°C.
• EZ-Link NHS-PEG4-Biotin, 8 x 2 mg
• PBS Pack (makes 500mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10mg
Store avidin and microtubes of biotin reagent at -20°C. Store PBS at room temperature. Store remaining kit components at 4°C.
Frequently asked questions (FAQs)
How do the EZ-Link Micro Biotinylation Kits differ from the standard biotinylation kits?
Can you provide the shelf-life for EZ-Link NHS-PEG4 Biotinylation Kit?
Citations & References (8)
Citations & References
Abstract
Single-Molecule Arrays for Ultrasensitive Detection of Blood-Based Biomarkers for Immunotherapy.
Journal:Methods Mol Biol
PubMed ID:31502162
'Single-molecule array (Simoa) technology enables ultrasensitive protein detection that is suited to the development of peripheral blood-based assays for assessing immuno-oncology responses. We adapted a panel of Simoa assays to measure systemic cytokine levels from plasma and characterized physiologic variation in healthy individuals and preanalytic variation arising from processing and
Designing antibodies against LRRK2-targeted tau epitopes.
Journal:PLoS One
PubMed ID:30261006
'Phosphorylation of the microtubule associated protein tau is an important modulator of its normal physiological functioning; however, it may also contribute to tau mis-folding and aggregation in neurodegenerative diseases, which are collectively termed tauopathies. As such, the investigations of tau phosphorylation and kinases that modify tau are important in trying
B Cells Improve Overall Survival in HPV-Associated Squamous Cell Carcinomas and Are Activated by Radiation and PD-1 Blockade.
Journal:Clin Cancer Res
PubMed ID:32193227
'To characterize the role of B cells on human papilloma virus (HPV)-associated cancer patient outcomes and determine the effects of radiation and PD-1 blockade on B-cell populations.'
A small molecule inhibitor of PCSK9 that antagonizes LDL receptor binding via interaction with a cryptic PCSK9 binding groove.
Journal:Bioorg Med Chem
PubMed ID:32051094
'Proprotein convertase (PC) subtilisin kexin type 9 (PCSK9) inhibits the clearance of low density lipoprotein (LDL) cholesterol from plasma by directly interacting with the LDL receptor (LDLR). As the interaction promotes elevated plasma LDL cholesterol levels and a predisposition to cardiovascular disease (CVD), it has attracted much interest as a
Gpr158 mediates osteocalcin's regulation of cognition.
Journal:J Exp Med
PubMed ID:28851741
That osteocalcin (OCN) is necessary for hippocampal-dependent memory and to prevent anxiety-like behaviors raises novel questions. One question is to determine whether OCN is also sufficient to improve these behaviors in wild-type mice, when circulating levels of OCN decline as they do with age. Here we show that the presence