Insulin-Transferrin-Selenium (ITS -G) (100X)

Citations & References (4)

Gibco™

Insulin-Transferrin-Selenium (ITS -G) (100X)

Gibco™ Insulin-Transferrin-Selenium (ITS-G) is used as a basal medium supplement in order to reduce the amount of fetal bovine serumRead more

Catalog Number	Quantity
41400045	10 mL

Catalog number 41400045

Price (CLP)

74.061

Add to cart

Quantity:

10 mL

Price (CLP)

74.061

Add to cart

Gibco™ Insulin-Transferrin-Selenium (ITS-G) is used as a basal medium supplement in order to reduce the amount of fetal bovine serum (FBS) needed to culture cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solution is now routinely used with other basal media to support a wide variety of cell types in the presence of 2–4% FBS.

Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide. Selenium, as sodium selenite, is a co-factor for glutathione peroxidase and other proteins, and is used as an anti-oxidant in media.

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco™ ITS-G is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell TypeMammalian Cells

Concentration100 X

Quantity10 mL

Shelf Life18 Months

Shipping ConditionRoom Temperature

FormLiquid

Product TypeTransferrin

SterilitySterile-filtered

Without AdditivesNo Phenol Red

Unit SizeEach

Contents & Storage

Storage conditions: 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 18 months from date of manufacture

Frequently asked questions (FAQs)

What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are all Insulin-Transferrin-Selenium (ITS) (100X) supplements offered by Thermo Fisher Scientific animal origin-free?

We offer both animal origin and animal origin-free versions of our ITS supplements. The animal origin-free product versions are referred to as ITS Select supplements.

Insulin-Transferrin-Selenium (ITS-G) (100X) (Cat. No. 41400045), Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (100X) (Cat. No. 51500056), and Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) are not animal origin-free.

Animal origin-free product versions are available for the ITS-G and ITS-X formulations: Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X) (Cat. No. A4000046501 and A4000046502) and Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Select Supplement (100X) (Cat. No. A4000046401 and Cat. No. A4000046402.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Insulin-Transferrin-Selenium (ITS -G) (100X) FAQs

Citations & References (4)

Citations & References

Abstract

A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.

Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T

Journal:Experimental cell research

PubMed ID:38499143

Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.

Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA

Journal:STAR protocols

PubMed ID:38833372

Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic âlivers in a dish.â We describe strategies to induce ... More

Collective cell migration requires suppression of actomyosin at cell-cell contacts mediated by DDR1 and the cell polarity regulators Par3 and Par6.

Authors:Hidalgo-Carcedo C, Hooper S, Chaudhry SI, Williamson P, Harrington K, Leitinger B, Sahai E,

Journal:Nat Cell Biol

PubMed ID:21170030

Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin is not downregulated at cell-cell contacts, migrating cells lose cohesion. ... More

Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology.

Authors:Smits AM, van Vliet P, Metz CH, Korfage T, Sluijter JP, Doevendans PA, Goumans MJ,

Journal:Nat Protoc

PubMed ID:19197267

To date, there is no suitable in vitro model to study human adult cardiac cell biology. Although embryonic stem cells are able to differentiate into cardiomyocytes in vitro, the efficiency of this process is very low. Other methods to differentiate progenitor cells into beating cardiomyocytes rely on coculturing with rat ... More