Leadmium™ Green AM dye

The proprietary Leadmium™ Green AM dye from Molecular Probes™ provides researchers with a unique tool to specifically measure intracellular leadRead more

Catalog Number	Quantity
A10024	5 x 50 μg

Catalog number A10024

Price (CLP)

Quantity:

5 x 50 μg

The proprietary Leadmium™ Green AM dye from Molecular Probes™ provides researchers with a unique tool to specifically measure intracellular lead and cadmium levels with high sensitivity. While standards assays exist for measuring lead and cadmium in solution, the unique Leadmium™ Green AM indicator was specifically designed to detect these toxic environmental contaminants inside living cells.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

–Highly specific indicator for intracellular measurements of lead and cadmium

–Flexible format allows use on multiple formats - flow cytometry, fluorescence microscopy imaging and plate readers
–Bright fluorescence and high discrimination from calcium allows detection of nanomolar levels of lead and micromolar levels of cadmium
–Convenient excitation with 488 nm laser line
–The Leadmium™ Green AM dye is readily loaded and is retained very well following esterase cleavage of the acetoxymethyl group

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeFluorescent Dye-Based

Quantity5 x 50 μg

Shipping ConditionRoom Temperature

For Use With (Equipment)Fluorescence Microscope, Flow Cytometer

Product LineLeadmium

Product TypeDye

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

Citations & References (4)

Citations & References

Abstract

A new ABC half-transporter in Leishmania major is involved in resistance to antimony.

Authors:Manzano JI, García-Hernández R, Castanys S, Gamarro F,

Journal:

PubMed ID:23716044

The characterization of ABCI4, a new intracellular ATP-binding cassette (ABC) half-transporter in Leishmania major, is described. We show that ABCI4 is involved in heavy metal export, thereby conferring resistance to Pentostam, to Sb(III), and to As(III) and Cd(II). Parasites overexpressing ABCI4 showed a lower mitochondrial toxic effect of antimony by ... More

Increased nuclear thioredoxin-1 potentiates cadmium-induced cytotoxicity.

Authors:Go YM, Orr M, Jones DP,

Journal:Toxicol Sci

PubMed ID:22961094

Cadmium (Cd) is a widely dispersed environmental agent that causes oxidative toxicity through mechanisms that are sensitive to thioredoxin-1 (Trx1). Trx1 is a cytoplasmic protein that translocates to nuclei during oxidative stress. Recent research shows that interaction of Trx1 with actin plays a critical role in cell survival and that ... More

Cellular sequestration of cadmium in the hyperaccumulator plant species Sedum alfredii.

Authors:Tian S, Lu L, Labavitch J, Yang X, He Z, Hu H, Sarangi R, Newville M, Commisso J, Brown P,

Journal:Plant Physiol

PubMed ID:22025609

Spatial imaging of cadmium (Cd) in the hyperaccumulator Sedum alfredii was investigated in vivo by laser ablation inductively coupled plasma mass spectrometry and x-ray microfluorescence imaging. Preferential Cd accumulation in the pith and cortex was observed in stems of the Cd hyperaccumulating ecotype (HE), whereas Cd was restricted to the ... More

Chemically diverse toxicants converge on Fyn and c-Cbl to disrupt precursor cell function.

Authors:Li Z, Dong T, Pröschel C, Noble M,

Journal:PLoS Biol

PubMed ID:17298174

Identification of common mechanistic principles that shed light on the action of the many chemically diverse toxicants to which we are exposed is of central importance in understanding how toxicants disrupt normal cellular function and in developing more effective means of protecting against such effects. Of particular importance is identifying ... More