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Invitrogen
BRD4 Recombinant Rabbit Monoclonal Antibody (1E5)
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FIGURE: 1 / 3
BRD4 Antibody (MA5-60646) in IHC (P)
Immunohistochemistry analysis of BRD4 in paraffin-embedded human breast cancer. Samples were incubated with BRD4 Recombinant Monoclonal antibody (Product # MA5-60646) using a dilution of 1:100 followed by detection with Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Sectio... View More
Product Details
MA5-60646
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
1E5
Immunogen
A synthesized peptide derived from human Brd4.
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Amount
80 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRD4-NUT FUSION; Bromodomain-containing protein 4; chromosome-associated protein; female sterile homeotic related gene 4; HUNKI; mitotic chromosome-associated protein; Protein HUNK1
Gene Aliases: BRD4; CAP; CDLS6; FSHRG4; HUNK1; HUNKI; MCAP
UniProt ID: (Human) O60885
Entrez Gene ID: (Human) 23476
transcription regulatory region sequence-specific DNA binding
p53 binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
protein serine/threonine kinase activity
protein binding
RNA polymerase II carboxy-terminal domain kinase activity
enzyme binding
protein binding, bridging
histone binding
RNA polymerase II C-terminal domain binding
P-TEFb complex binding
histone H4 reader activity
histone H4K12ac reader activity
histone H4K5ac reader activity
histone H4K16ac reader activity
histone H4K8ac reader activity
histone H3K9ac reader activity
histone H3K27ac reader activity
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation by host of viral transcription
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
negative regulation of DNA damage checkpoint
positive regulation of T-helper 17 cell lineage commitment
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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