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Invitrogen

MDM2 Monoclonal Antibody (IF2)

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View all (106) MDM2 antibodies

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MDM2 Monoclonal Antibody (IF2)

Product Details

18-2403

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Clone

IF2

Immunogen

Synthetic peptide derived from the n-terminal region of human MDM2
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS with 1% BSA

Contains

0.1% sodium azide

Storage conditions

2-8°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2925246

Product Specific Information

This monoclonal antibody reacts specifically with human MDM2 protein. Overexpression of MDM2 protein has been detected in a variety of human tumors, and appears to result from gene amplification, increased transcript level, and enhanced translation. Reactivity with mouse MDM2 protein has not been observed.

Expected Staining Pattern: Nuclear

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alternatively spliced isoform; Double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HGNC:6973; human homolog of; MDM2 isoform KB9; MDM2 isoform N1_40; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53 associated; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; unnamed protein product

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Gene Aliases: ACTFS; hdm2; HDMX; LSKB; MDM2

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UniProt ID: (Human) Q00987

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Entrez Gene ID: (Human) 4193

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Function(s)
p53 binding ubiquitin-protein transferase activity protein binding 5S rRNA binding zinc ion binding transferase activity ligase activity SUMO transferase activity enzyme binding protein domain specific binding ubiquitin protein ligase binding receptor serine/threonine kinase binding identical protein binding peroxisome proliferator activated receptor binding ribonucleoprotein complex binding ubiquitin binding metal ion binding ubiquitin protein ligase activity NEDD8 ligase activity disordered domain specific binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination blood vessel development blood vessel remodeling regulation of heart rate heart valve development atrioventricular valve morphogenesis endocardial cushion morphogenesis ventricular septum development atrial septum development ubiquitin-dependent protein catabolic process apoptotic process traversing start control point of mitotic cell cycle heart development positive regulation of cell proliferation response to xenobiotic stimulus response to toxic substance response to iron ion regulation of gene expression positive regulation of gene expression negative regulation of gene expression negative regulation of protein processing negative regulation of neuron projection development protein ubiquitination protein sumoylation DNA damage response, signal transduction by p53 class mediator protein destabilization response to magnesium ion positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein localization to nucleus regulation of protein catabolic process response to cocaine negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of DNA damage response, signal transduction by p53 class mediator protein-containing complex subunit organization establishment of protein localization response to ether positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of mitotic cell cycle response to antibiotic positive regulation of protein export from nucleus response to steroid hormone positive regulation of muscle cell differentiation proteolysis involved in cellular protein catabolic process regulation of cell cycle protein autoubiquitination cardiac septum morphogenesis protein-containing complex assembly regulation of biological quality cellular response to hydrogen peroxide cellular response to antibiotic cellular response to vitamin B1 cellular response to alkaloid cellular response to growth factor stimulus cellular response to peptide hormone stimulus cellular response to estrogen stimulus cellular response to hypoxia cellular response to gamma radiation cellular response to UV-C signal transduction by p53 class mediator fibroblast activation cellular response to actinomycin D regulation of postsynaptic neurotransmitter receptor internalization regulation of protein catabolic process at postsynapse, modulating synaptic transmission negative regulation of signal transduction by p53 class mediator negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator response to formaldehyde positive regulation of vascular smooth muscle cell proliferation positive regulation of vascular associated smooth muscle cell migration amyloid fibril formation response to water-immersion restraint stress negative regulation of apoptotic signaling pathway
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