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Invitrogen
Phospho-PAX2 (Ser393) Polyclonal Antibody
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FIGURE: 1 / 5
Phospho-PAX2 (Ser393) Antibody (36-9200) in IHC (P)
Immunohistochemistry analysis of Phospho-Pax2 (pSer393) showing nucleus and weak cytoplasmic staining of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 1... View More
Product Details
36-9200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2). Ser393 of the 415 amino acid mouse Pax2 isoform is equivalent to Ser394 of the 416 amino acid human Pax2 isoform, and to Ser371 of the 393 amino acid human Pax2 isoform.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: optic disc coloboma; paired box gene 2; paired box homeotic gene 2; Paired box protein Pax-2
Gene Aliases: FSGS7; Opdc; PAPRS; PAX-2; PAX2
UniProt ID: (Human) Q02962, (Mouse) P32114
Entrez Gene ID: (Human) 5076, (Mouse) 18504, (Rat) 293992
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
sequence-specific double-stranded DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
superoxide-generating NADPH oxidase activity
transcription regulatory region DNA binding
C2H2 zinc finger domain binding
sequence-specific DNA binding
urogenital system development
branching involved in ureteric bud morphogenesis
cell fate determination
mesonephros development
neural tube closure
optic cup morphogenesis involved in camera-type eye development
mesenchymal to epithelial transition involved in metanephros morphogenesis
retinal pigment epithelium development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
nervous system development
axonogenesis
sensory organ development
mesodermal cell fate specification
visual perception
glial cell differentiation
optic nerve development
optic nerve morphogenesis
optic nerve structural organization
vestibulocochlear nerve formation
cell differentiation
regulation of metanephros size
ureter maturation
pronephric field specification
inner ear morphogenesis
camera-type eye development
negative regulation of apoptotic process
negative regulation of programmed cell death
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
animal organ development
system development
pronephros development
brain morphogenesis
stem cell differentiation
positive regulation of epithelial cell proliferation
mesenchymal to epithelial transition
optic chiasma development
cellular response to retinoic acid
cellular response to glucose stimulus
metanephric mesenchyme development
positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
metanephric mesenchymal cell differentiation
nephric duct formation
ureter development
metanephric collecting duct development
metanephric epithelium development
metanephric distal convoluted tubule development
metanephric nephron tubule formation
positive regulation of metanephric glomerulus development
negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis
regulation of metanephric nephron tubule epithelial cell differentiation
cochlea development
cochlea morphogenesis
negative regulation of mesenchymal cell apoptotic process involved in metanephros development
negative regulation of apoptotic process involved in metanephric collecting duct development
negative regulation of apoptotic process involved in metanephric nephron tubule development
negative regulation of reactive oxygen species metabolic process
positive regulation of metanephric DCT cell differentiation
positive regulation of optic nerve formation
negative regulation of transcription from RNA polymerase II promoter
metanephros development
ureteric bud development
kidney development
transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
positive regulation of cell proliferation
regulation of apoptotic process
regulation of programmed cell death
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein kinase B signaling
negative regulation of cytolysis
oxidation-reduction process
paramesonephric duct development
renal system development
mesonephric tubule development
mesonephric tubule formation
mesonephric duct development
reactive oxygen species metabolic process
positive regulation of branching involved in ureteric bud morphogenesis
aging
pancreas development
response to nutrient levels
cellular response to hydrogen peroxide
cellular response to mechanical stimulus
cellular response to epidermal growth factor stimulus
ureter morphogenesis
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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