Invitrogen™

PrimeFlow™ Fixation/Permeabilization Buffer Set

El juego de tampones de fijación/permeabilización PrimeFlow™ es un componente del kit de ensayo de ARN PrimeFlow™ (n.° de referenciaMás información

Número de catálogo	Cantidad
88-17000-210	100 pruebas

Número de catálogo 88-17000-210

Precio (CLP)

590.909

Cantidad:

100 pruebas

Precio (CLP)

590.909

El juego de tampones de fijación/permeabilización PrimeFlow™ es un componente del kit de ensayo de ARN PrimeFlow™ (n.° de referencia 88-18001-204 y 88-18009-204). Estos tampones se pueden utilizar para evaluar el rendimiento del anticuerpo de tinción intracelular siguiendo los pasos de fijación y permeabilidad del ensayo. Tenga en cuenta que se pueden observar cambios adicionales en el rendimiento del anticuerpo después de los pasos de hibridación del ensayo de ARN PrimeFlow™.

Consulte el protocolo del ensayo de ARN PrimeFlow™ en la hoja de datos del producto para obtener instrucciones de uso.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Para utilizar con (aplicación)Tinción intracelular seguida de análisis mediante citometría de flujo

Para utilizar con (equipo)Citómetros de flujo

N.º de reacciones100 pruebas

Tipo de productoConjunto de tampón para ensayo de ARN

Cantidad100 pruebas

Unit SizeEach

Contenido y almacenamiento

De 2 °C a 8 °C

Preguntas frecuentes

When running the QuantiGene FlowRNA (methanol based) assay, which fluorochromes are recommended for antibody staining?

We recommend the use of fluorochromes that are known to be methanol resistant. Avoid protein-based fluorochromes such as APC, PE, and PerCP and their respective tandems. This question is not appropriate for PrimeFlow RNA Assay.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For the PrimeFlow RNA Assay, how would you recommend I set compensation?

We recommend using the PrimeFlow Compensation Kit included in the PrimeFlow RNA Assay (Cat. No. 88-18005-204, 88-18005-210) to set compensation for each RNA channel. Refer to Appendix 3 of the PrimeFlow RNA Assay User Manual for detailed instructions for use. The UltraComp eBeads microspheres included in the compensation kit may also be used for any experimental antibodies being used. Antibodies with the same fluorochromes as the RNA probes should not be used to set compensation for RNA probes, as the compensation values will be different.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have a 37 degrees C tissue culture incubator in the lab. Can I use this incubator for the hybridization steps in the PrimeFlow RNA Assay protocol?

The hybridization temperature is a critical parameter of this protocol necessary to obtain positive results. The incubator to be used must be validated before use with the ViewRNA Temperature Validation Kit (Cat. No. QV0523), following the protocol in the FlowRNA user manual. Temperatures deviated outside of this range will lead to less-than-optimal hybridization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of 96-well plate is compatible with the PrimeFlow RNA protocol?

We recommend polystyrene uncoated v-bottom 96-well plates with a lid (Cat. No. 44-17005-46). The u-bottom plate is also acceptable, but flat bottom plates are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How many cells should I use per well for the 96-well format of the PrimeFlow RNA Assay?

We recommend 1-5 million cells in 100 µL of Flow Cytometry Staining Buffer per well. The starting cell number is critical for maximal signal intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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