Mini-Gel-Tank

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Yes, NativePAGE gels are compatible with our Mini Gel tank, however there is a small variation from the original protocol.

The following protocol are for using NativePage gels with the Mini Gel Tank depending on if you are also performing a western transfer or not:
If you are NOT performing a western transfer:
1. Prepare 250 mL of each buffer (Anode and Dark Blue Cathode) per gel
2. After inserting a loaded NativePAGE gel into the Mini Gel Tank and pulling the clamp forward, fill the front cathode buffer chamber to the Fill Line with Dark Blue Cathode Buffer (~200 mL per gel)
3. Add 220 mL of Anode Buffer to the back anode buffer chamber for that gel. Start the gel run

If you are performing a western transfer:
1. Prepare 250 mL of Dark Blue Cathode Buffer, 250 mL of Light Blue Cathode Buffer, and 500 mL of Anode Buffer per gel
2. After inserting a loaded NativePAGE gel into the Mini Gel Tank and pulling the clamp forward, fill the front cathode buffer chamber to the Fill Line with Dark Blue Cathode Buffer (~200 mL per gel
3. Add 220 mL of Anode Buffer to the back anode buffer chamber for that gel
4. Start the gel run; pause the run after the dark blue dye has run ~1/3 of the way through gel
a. Pour out the buffers from the Mini Gel Tank
b. Refill the back anode buffer chamber with 220 mL of Anode Buffer per gel
c. Fill the front cathode buffer chamber to the Fill Line with Light Blue Cathode Buffer (~200 mL per gel)
5. Resume the gel run

Find additional tips, troubleshooting help, and resources within our Protein Biology Support Centers .