FAST DiI™ oil; DiIΔ9,12-C18(3), ClO4 (1,1'-Dilinoleyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)
<i>FAST</i> DiI&trade; oil; DiI&Delta;<sup>9,12</sup>-C<sub>18</sub>(3), ClO<sub>4</sub> (1,1'-Dilinoleyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)
Citas y referencias (42)
Invitrogen™

FAST DiI™ oil; DiIΔ9,12-C18(3), ClO4 (1,1'-Dilinoleyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)

FAST DiI es an analógico insaturado de DiI (DiIC18(3)) que migra un ∼50 % más rápido. Es débilmente fluorescente enMás información
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Número de catálogoCantidad
D38995 mg
Número de catálogo D3899
Precio (CLP)
382.179
Each
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Cantidad:
5 mg
Precio (CLP)
382.179
Each
Añadir al carro de la compra
FAST DiI es an analógico insaturado de DiI (DiIC18(3)) que migra un ∼50 % más rápido. Es débilmente fluorescente en agua, pero muy fluorescente y bastante fotoestable cuando se incorpora a membranas. Tiene un coeficiente de extinción extremadamente alto y tiempos de vida de estado excitado cortos (∼ 1 nanosegundo) en entornos lipídicos. Una vez aplicado a las células, el colorante se difunde lateralmente dentro de la membrana plasmática.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorAmarillo
Método de detecciónFluorescente
Para utilizar con (equipo)Microscopio de fluorescencia
Línea de productosFAST DiI
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoDiIΔ9 12-C18(3) ClO4
SubCellular LocalizationMembranas celulares y lípidos, Lipids
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

Lipophilic cyanine dyes are preferred for this sort of assay, since they insert into cellular membranes and then, upon fusion, are shared by the fused cells as the membranes are shared. For example, one cell population can be labeled with DiI (orange-red) and another cell population can be labeled with DiO (green), and when the cells fuse, the combined color appears yellow (when imaged with a dual-bandpass filter set).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (42)

Citations & References
Abstract
Reticulated lipid probe fluorescence reveals MDCK cell apical membrane topography.
Authors:Colarusso P, Spring KR
Journal:Biophys J
PubMed ID:11806917
High spatial resolution confocal microscopy of young MDCK cells stained with the lipophilic probe 1,1'-dihexadecyl-3,3,3',3'- tetramethylindocarbocyanine perchlorate (DiIC(16)) revealed a reticulated fluorescence pattern on the apical membrane. DiIC(16) was delivered as crystals to live cells to minimize possible solvent perturbations of the membrane lipids. The ratio of the integrated fluorescence ... More
Involvement of raft-like plasma membrane domains of Entamoeba histolytica in pinocytosis and adhesion.
Authors:Laughlin RC, McGugan GC, Powell RR, Welter BH, Temesvari LA
Journal:Infect Immun
PubMed ID:15322032
'Lipid rafts are highly ordered, cholesterol-rich, and detergent-resistant microdomains found in the plasma membrane of many eukaryotic cells. These domains play important roles in endocytosis, secretion, and adhesion in a variety of cell types. The parasitic protozoan Entamoeba histolytica, the causative agent of amoebic dysentery, was determined to have raft-like ... More
Growth-associated protein-43 is required for commissural axon guidance in the developing vertebrate nervous system.
Authors:Shen Y, Mani S, Donovan SL, Schwob JE, Meiri KF
Journal:J Neurosci
PubMed ID:11756507
'Growth-associated protein-43 (GAP-43) is a major growth cone protein whose phosphorylation by PKC in response to extracellular guidance cues can regulate F-actin behavior. Here we show that 100% of homozygote GAP-43 (-/-) mice failed to form the anterior commissure (AC), hippocampal commissure (HC), and corpus callosum (CC) in vivo. Instead, ... More
Use of lipophilic dyes in studies of axonal pathfinding in vivo.
Authors:Perrin FE, Stoeckli ET
Journal:Microsc Res Tech
PubMed ID:10620782
'Fluorescent lipophilic dyes are an ideal tool to study axonal pathfinding. Because these dyes do not require active axonal transport for their spreading, they can be used in fixed tissue. Here, we describe the method we have used to study the molecular mechanisms of commissural axon pathfinding in the embryonic ... More
Differential projections of ciliated and microvillous olfactory receptor cells in the catfish, Ictalurus punctatus.
Authors:Morita Y, Finger TE
Journal:J Comp Neurol
PubMed ID:9717708
'The primary olfactory projections of channel catfish Ictalurus punctatus have been examined with postmortem tracing by using either 1,1&#39;-dioctadecyl-3,3,3&#39;,3&#39;-tetramethylindocarbocyanine perchlorate or 1,1-dilinoleyl-3,3,3&#39;,3&#39;-tetramethylindocarbocyanine perchlorate (DiI). Following DiI deposition into small areas in different parts of the posterior olfactory bulb, olfactory sensory neurons always were labeled throughout the olfactory epithelium. No obvious ... More
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