XTT (2,3-bis-(2-metoxi-4-nitro-5-sulfofenil)-2H-tetrazolio-5-carboxanilida)

XTT (2,3-bis-(2-metoxi-4-nitro-5-sulfofenil)-2<i>H</i>-tetrazolio-5-carboxanilida)

Citas y referencias (62)

Invitrogen™

XTT (2,3-bis-(2-metoxi-4-nitro-5-sulfofenil)-2H-tetrazolio-5-carboxanilida)

El XTT se utiliza para evaluar la viabilidad celular como función del potencial redox. Las células que respiran activamente conviertenMás información

Número de catálogo	Cantidad
X6493	100 mg

Número de catálogo X6493

Precio (CLP)

237.624

100 mg

Añadir al carro de la compra

Cantidad:

100 mg

Precio (CLP)

237.624

100 mg

Añadir al carro de la compra

El XTT se utiliza para evaluar la viabilidad celular como función del potencial redox. Las células que respiran activamente convierten el XTT hidrosoluble en un producto de formazán de color naranja hidrosoluble. A diferencia de MTT, XTT no requiere solubilización antes de la cuantificación, lo que reduce el tiempo de ensayo en muchos protocolos de ensayo de viabilidad. Además, se ha informado de que la sensibilidad del ensayo de reducción de XTT es similar o superior a la del ensayo de reducción de MTT.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaCélulas eucariotas

Método de detecciónColorimétrico

Tipo de coloranteOtras etiquetas o colorantes

FormatoPlaca de 96 pocillos

Cantidad100 mg

Condiciones de envíoTemperatura ambiente

Para utilizar con (equipo)Lector de microplacas

Tipo de productoXTT

Unit Size100 mg

Contenido y almacenamiento

Almacenar en congelador entre -5 °C y -30 °C

Preguntas frecuentes

MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

If I used XTT (Cat. No. X6493) with an electron coupling reagent, would I get the same results as if I used the CyQUANT XTT Cell Viability Assay kit (Cat. No. X12223)?

Both products use the same reagent, XTT. In theory, the results of any optimized assay using XTT (Cat. No. X6493) may be comparable to using the CyQUANT XTT Cell Viability Assay kit (Cat. No. X12223). Variations may arise due to the type of electron coupling reagent used, the final concentration of XTT, and any assay-specific requirements of the solutions used in resolubilizing the reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When should I use the XTT reagent alone (Cat No. X6493) versus the CyQUANT XTT Cell Viability Assay (Cat No. X12223)?

For the CyQUANT XTT Cell Viability Assay kit (Cat No. X12223), the amount of reagents, the formulation of the solutions, and the identity of the Electron Coupling Reagent are proprietary information. On the other hand, XTT (Cat. No. X6493) may be resolubilized in either water, buffer, media, or DMSO to a desired stock concentration. We would recommend using XTT, Cat No. X6493 if you need to optimize the final working concentration, if the type of buffer/solvent used to resolubilize the reagent is critical to the study, if you elect not to use an electron coupling reagent, or if you'd like to use a known electron coupling reagent of a defined concentration.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between XTT (Cat No. X6493) and CyQUANT XTT Cell Viability Assay (Cat No. X12223)?

XTT (Cat No. X6493) is 100 mg of XTT in solid form; you must resolubilize this into an aqueous solution or DMSO prior to use.

CyQUANT XTT Cell Viability Assay (Cat No. X12223) is a kit that provides two components: 10 vials each of the XTT Reagent and Electron Coupling Reagent. Both kit components are in solution in a ready-to-use format; mix 1 vial of the XTT Reagent and 1 vial of the Electron Coupling Reagent immediately before use.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (62)

Citations & References

Abstract

Endoglin is a component of the transforming growth factor (TGF)-beta receptor complex of human pre-B leukemic cells.

Authors:Zhang H, Shaw AR, Mak A, Letarte M

Journal:J Immunol

PubMed ID:8543807

'Endoglin was first identified on a cell line derived from pre-B acute lymphoblastic leukemia. This 180-kDa homodimeric glycoprotein was then shown to be primarily expressed on endothelial cells and to bind the beta 1 and beta 3 isoforms of TGF-beta with high affinity. We now demonstrate that pre-B leukemic cells ... More

9-Nitrocamptothecin inhibits HIV-1 replication in human peripheral blood lymphocytes: a potential alternative for HIV-infection/AIDS therapy.

Authors:Hung CL, Doniger J, Palini A, Snyder SW, Radonovich MF, Brady JN, Pantazis P, Sadaie MR

Journal:J Med Virol

PubMed ID:11424110

'The ability of the anti-cancer drug, 9-Nitrocamptothecin (9NC), to inhibit replication of HIV-1 in clinically relevant primary lymphocytic cells was studied. Primary peripheral blood lymphocytes (PBLs) from a non-infected donor were freshly infected with HIV-1 and treated with 9NC by using three different treatment schedules. Cells were monitored for cytotoxicity ... More

In vitro effects of antiviral agents on human keratocytes.

Authors:Moreira LB, Oliveira C, Seitz B, LaBree L, McDonnell PJ

Journal:Cornea

PubMed ID:11189008

'PURPOSE: To study the effects of antiviral agents on human keratocytes in vitro. METHODS: Cultured human keratocytes were incubated with either ganciclovir, idoxuridine, trifluridine, or cidofovir at concentrations from 0.0001 to 10 mg/mL. Phase-contrast microscopy and XTT (sodium [2,3-bis [2-methoxy-4-nitro-5-sulphophenyl]-2h-tetrazolium-5-carboxanilide, inner salt) colorimetric assay were performed after 24, 48, and ... More

The interaction of human monocytes, monocyte-derived macrophages, and polymorphonuclear neutrophils with caspofungin (MK-0991), an echinocandin, for antifungal activity against Aspergillus fumigatus.

Authors:Chiller T, Farrokhshad K, Brummer E, Stevens DA

Journal:Diagn Microbiol Infect Dis

PubMed ID:11248522

'The collaboration between human effector cells and caspofungin (MK-0991), a 1,3-beta-D glucan synthase inhibitor, was studied for antifungal activity against Aspergillus fumigatus. Caspofungin was co-cultured for 24h with either human monocytes (Monos), monocyte-derived macrophages (MDM), or polymorphonuclear neutrophils (PMN) against germlings of A. fumigatus and antifungal activity assessed using the ... More

In vitro growth and analysis of Candida biofilms.

Authors:Chandra J, Mukherjee PK, Ghannoum MA,

Journal:Nat Protoc

PubMed ID:19180075

'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion ... More

View all XTT (2,3-bis-(2-metoxi-4-nitro-5-sulfofenil)-2H-tetrazolio-5-carboxanilida) citations