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Citas y referencias (17)
Invitrogen™
DQ™ Red BSA - Special Packaging
BSA DQ Red es un sustrato fluorogénico para las proteasas. Se observa un fuerte efecto de supresión de fluorescencia cuandoMás información
| Número de catálogo | Cantidad |
|---|---|
| D12051 | 5 x 1 mg |
Número de catálogo D12051
Precio (MXN)
-
Cantidad:
5 x 1 mg
BSA DQ Red es un sustrato fluorogénico para las proteasas. Se observa un fuerte efecto de supresión de fluorescencia cuando las proteínas están fuertemente marcadas con tintes BODIPY. Tras la hidrólisis de BSA DQ Red a péptidos únicos marcados con tinte por proteasas, esta supresión se alivia, lo que produce productos fluorescentes brillantes.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Línea de productosDQ
Cantidad5 x 1 mg
Condiciones de envíoTemperatura ambiente
SustratoSustrato de proteasa
Método de detecciónFluorescente
FormularioSólido
Substrate PropertiesSustrato basado en proteínas
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
Citations & References (17)
Citations & References
Abstract
Higher order Rab programming in phagolysosome biogenesis.
Journal:J Cell Biol
PubMed ID:16982798
'Phagosomes offer kinetically and morphologically tractable organelles to dissect the control of phagolysosome biogenesis by Rab GTPases. Model phagosomes harboring latex beads undergo a coordinated Rab5-Rab7 exchange, which is akin to the process of endosomal Rab conversion, the control mechanisms of which are unknown. In the process of blocking phagosomal
Human IRGM induces autophagy to eliminate intracellular mycobacteria.
Journal:Science
PubMed ID:16888103
Immunity-related p47 guanosine triphosphatases (IRG) play a role in defense against intracellular pathogens. We found that the murine Irgm1 (LRG-47) guanosine triphosphatase induced autophagy and generated large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. We also identified a function for a human IRG protein in
Assays to assess autophagy induction and fusion of autophagic vacuoles with a degradative compartment, using monodansylcadaverine (MDC) and DQ-BSA.
Journal:Methods Enzymol
PubMed ID:19200877
In this chapter we describe the use of monodasylcadaverine (MDC) and DQ-BSA, two practical and convenient tools to study the autophagic pathway. MDC is a lysosomotropic compound useful for the identification of autophagic vesicles by fluorescence microscopy and, in addition, to assess autophagy induction via the accumulation of MDC-labeled vacuoles.
Immune complex relay by subcapsular sinus macrophages and noncognate B cells drives antibody affinity maturation.
Journal:Nat Immunol
PubMed ID:19503106
Subcapsular sinus (SCS) macrophages capture antigens from lymph and present them intact for B cell encounter and follicular delivery. However, the properties of SCS macrophages are poorly defined. Here we show SCS macrophage development depended on lymphotoxin-alpha1beta2, and the cells had low lysosomal enzyme expression and retained opsonized antigens on
In vivo visualization of albumin degradation in the proximal tubule.
Journal:Kidney Int
PubMed ID:18800029
Albuminuria is a key marker of renal injury and a major risk factor for cardiovascular disease. In vivo imaging techniques with fluorescent albumin have allowed visualization of its movement within the whole kidney but they could not distinguish between intact and degraded albumin. To visualize albumin degradation in proximal tubular