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Proteintech
RUNX2 Recombinant Rabbit Monoclonal Antibody (1G5)
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Product Details
82636-2-RR
Applications
Tested Dilution
Publications
Immunoprecipitation (IP)
0.5-4.0 µg for 1.0-3.0 mg of total protein lysate
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
CHO cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1G5
Immunogen
Peptide
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Target Information
RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acute myeloid leukemia 3 protein; AKV core binding factor; AML-3; CBF-alpha-1; core binding factor alpha 1; Core-binding factor subunit alpha-1; core-binding factor, runt domain, alpha subunit 1; Oncogene AML-3; OSF-2; Osteoblast-specific transcription factor 2; PEA2-alpha A; PEBP2 alpha A; PEBP2-alpha A; Polyomavirus enhancer-binding protein 2 alpha A subunit; runt domain, alpha subunit 1; runt related transcription factor 2; Runt-related transcription factor 2; RUNX 2; RUNX-2; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
Gene Aliases: AML3; Cbf; CBF-alpha-1; Cbfa-1; CBFA1; CCD; CCD1; CLCD; LS3; OSF-2; OSF2; PEA2aA; PEBP2A; Pebp2a1; PEBP2aA; Pebpa2a; RUNX2
UniProt ID: (Human) Q13950, (Mouse) Q08775
Entrez Gene ID: (Human) 860, (Mouse) 12393
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
ATP binding
protein domain specific binding
chromatin DNA binding
bHLH transcription factor binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor binding
histone deacetylase binding
transcription regulatory region DNA binding
repressing transcription factor binding
Runt transcription factor
skeletal system development
ossification
osteoblast differentiation
endochondral ossification
osteoblast fate commitment
chondrocyte differentiation
chondrocyte development
osteoblast development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transmembrane receptor protein serine/threonine kinase signaling pathway
smoothened signaling pathway
cell proliferation
positive regulation of cell proliferation
gene expression
regulation of gene expression
positive regulation of gene expression
hemopoiesis
cell differentiation
neuron differentiation
T cell differentiation
regulation of ossification
bone mineralization
BMP signaling pathway
positive regulation of chondrocyte differentiation
embryonic forelimb morphogenesis
ligamentous ossification
regulation of fibroblast growth factor receptor signaling pathway
odontogenesis of dentin-containing tooth
regulation of odontogenesis of dentin-containing tooth
regulation of cell differentiation
regulation of osteoblast differentiation
positive regulation of osteoblast differentiation
negative regulation of smoothened signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell maturation
embryonic cranial skeleton morphogenesis
skeletal system morphogenesis
stem cell differentiation
epithelial cell proliferation
positive regulation of epithelial cell proliferation
SMAD protein signal transduction
stem cell proliferation
response to sodium phosphate
positive regulation of stem cell proliferation
transcription, DNA-templated
transcription from RNA polymerase II promoter
positive regulation of ossification
cellular response to BMP stimulus
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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