Search
Citations & References (7)
Thermo Scientific™
Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sampleRead more
Have Questions?
Change view
| Catalog Number | Quantity |
|---|---|
| 78446 | 10 mL |
| 78442 | 24 x 100 μL |
| 78440 | 1 mL |
| 78444 | 5 x 1 mL |
Catalog number 78446
Price (EUR)
1.082,65
Online Exclusive
1.186,00Save 103,35 (9%)
Each
Quantity:
10 mL
Price (EUR)
1.082,65
Online Exclusive
1.186,00Save 103,35 (9%)
Each
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sample protection for cell and tissue lysates.
Features of Halt Protease and Phosphatase Inhibitor Cocktail (100X):
• Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors
• Multiple package sizes—choose from four convenient packages sizes to match nearly any scale
• Convenient —the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
• Easy storage—aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
• Compatible—can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
• Validated—fully compatible with Thermo Scientific Pierce Cell Lysis Buffers
Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. To promote the inhibition of metalloproteases, 0.5M EDTA is provided in a separate tube.
Related Products
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Features of Halt Protease and Phosphatase Inhibitor Cocktail (100X):
• Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors
• Multiple package sizes—choose from four convenient packages sizes to match nearly any scale
• Convenient —the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
• Easy storage—aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
• Compatible—can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
• Validated—fully compatible with Thermo Scientific Pierce Cell Lysis Buffers
Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. To promote the inhibition of metalloproteases, 0.5M EDTA is provided in a separate tube.
Related Products
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferLysis Buffer
InhibitorsProtease Inhibitor, Phosphatase Inhibitor
Quantity10 mL
Reagent TypeProtease Inhibitor, Phosphatase Inhibitor
FormLiquid
Product LineHalt
Product TypeProtease and Phosphatase Inhibitor Cocktail
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C
Frequently asked questions (FAQs)
Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?
What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?
Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?
When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?
How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?
Citations & References (7)
Citations & References
Abstract
A guide to selecting high-performing antibodies for human Midkine for use in Western blot and immunoprecipitation.
Journal:F1000Research
PubMed ID:39092005
Midkine is a secreted protein that acts as a growth factor or cytokine involved in cell survival and inflammatory processes. It accumulates in amyloid plaques, which are hallmarks of Alzheimer's Disease (AD). The reproducibility of Midkine research would be enhanced if the community had access to well-characterized anti-Midkine antibodies. In
Chemoproteomic discovery of a covalent allosteric inhibitor of WRN helicase.
Journal:Nature
PubMed ID:38658751
WRN helicase is a promising target for treatment of cancers with microsatellite instability (MSI) due to its essential role in resolving deleterious non-canonical DNA structures that accumulate in cells with faulty mismatch repair mechanisms(1-5). Currently there are no approved drugs directly targeting human DNA or RNA helicases, in part owing
Selection of Leptin Surrogates by a General Phenotypic Screening Method for Receptor Agonists.
Journal:Biomolecules
PubMed ID:38672473
There is a high demand for agonist biomolecules such as cytokine surrogates in both biological and medicinal research fields. These are typically sourced through natural ligand engineering or affinity-based screening, followed by individual functional validation. However, efficient screening methods for identifying rare hits within immense libraries are very limited. In
hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels.
Journal:J Biol Chem
PubMed ID:15919655
'Proteins of the CLCA gene family have been proposed to mediate calcium-activated chloride currents. In this study, we used detailed bioinformatics analysis and found that no transmembrane domains are predicted in hCLCA1 or mCLCA3 (Gob-5). Further analysis suggested that they are globular proteins containing domains that are likely to be
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes.