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Citations & References (11)
Invitrogen™
Alexa Fluor™ 350 Hydrazide
Alexa Fluor™ 350 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketonesRead more
| Catalog Number | Quantity |
|---|---|
| A10439 also known as A-10439 | 5 mg |
Catalog number A10439
also known as A-10439
Price (EUR)
554,00
Each
Quantity:
5 mg
Price (EUR)
554,00
Each
Alexa Fluor™ 350 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 350 is a blue fluorescent dye with excitation ideally suited to the UV laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 350 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor™ 350 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection (learn more).
Detailed information about this AlexaFluor™ hydrazide:
• Fluorophore label : Alexa Fluor™ 350 dye
• Reactive group: hydrazide
• Reactivity: Aldehydes or keytones in polysaccharides or glycoproteins
• Ex/Em of the conjugate: 345/445 nm
• Extinction coefficient: 13,000 cm-1M-1
• Spectrally similar dyes: DAPI
• Molecular weight: 349.29
Cell Tracking and Tracing Applications
Alexa Fluor™ hydrazides and hydroxlamines are useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent. Learn more about cell tracking and tracing.
Glycoprotein and Polysaccharide Labeling Applications
The Alexa Fluor™ hydrazides and hydroxlamines are reactive molecules that can be used to add a fluorescent label to biomolecules containing aldehydes or ketones. Aldehydes and ketones can be introduced into polysaccharides and glycoproteins by periodate-mediated oxidation of vicinal diols. Galactose oxidase can also be used to oxidize terminal galactose residues of glycoproteins to aldehydes.
Hydrazide vs Hydroxylamine
Hydrazine derivatives react with ketones and aldehydes to yield relatively stable hydrazones. Hydroxylamine derivatives (aminooxy compounds) react with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. Both hydrazones and oximes can be reduced with sodium borohydride (NaBH4) to further increase the stability of the linkage.
Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices. To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in The Molecular Probes™ Handbook.
We’ll Make a Custom Conjugate for You
If you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.
Related Products
DMSO (dimethylsulfoxide) (D12345)
Antibody Conjugate Purification Kit for 0.5-1 mg (A33086)
Antibody Conjugate Purification Kit for 20-50 μg (A33087)
Antibody Conjugate Purification kit for 50-100 μg (A33088)
Detailed information about this AlexaFluor™ hydrazide:
• Fluorophore label : Alexa Fluor™ 350 dye
• Reactive group: hydrazide
• Reactivity: Aldehydes or keytones in polysaccharides or glycoproteins
• Ex/Em of the conjugate: 345/445 nm
• Extinction coefficient: 13,000 cm-1M-1
• Spectrally similar dyes: DAPI
• Molecular weight: 349.29
Cell Tracking and Tracing Applications
Alexa Fluor™ hydrazides and hydroxlamines are useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent. Learn more about cell tracking and tracing.
Glycoprotein and Polysaccharide Labeling Applications
The Alexa Fluor™ hydrazides and hydroxlamines are reactive molecules that can be used to add a fluorescent label to biomolecules containing aldehydes or ketones. Aldehydes and ketones can be introduced into polysaccharides and glycoproteins by periodate-mediated oxidation of vicinal diols. Galactose oxidase can also be used to oxidize terminal galactose residues of glycoproteins to aldehydes.
Hydrazide vs Hydroxylamine
Hydrazine derivatives react with ketones and aldehydes to yield relatively stable hydrazones. Hydroxylamine derivatives (aminooxy compounds) react with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. Both hydrazones and oximes can be reduced with sodium borohydride (NaBH4) to further increase the stability of the linkage.
Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices. To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in The Molecular Probes™ Handbook.
We’ll Make a Custom Conjugate for You
If you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.
Related Products
DMSO (dimethylsulfoxide) (D12345)
Antibody Conjugate Purification Kit for 0.5-1 mg (A33086)
Antibody Conjugate Purification Kit for 20-50 μg (A33087)
Antibody Conjugate Purification kit for 50-100 μg (A33088)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityCarboxylic Acid, Ketone, Aldehyde
Emission445 nm
Excitation345 nm
Label or DyeAlexa Fluor™ 350
Product TypeHydrazide
Quantity5 mg
Reactive MoietyAmine, Hydrazide
Shipping ConditionRoom Temperature
Label TypeAlexa Fluor
Product LineAlexa Fluor
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
Frequently asked questions (FAQs)
What dye can I use that is non-reactive and can show an injection site?
I injected a fluorescent tracer, but cannot detect it after tissue is fixed and sectioned. What am I doing wrong?
I have labeled my neurons with an Alexa Fluor conjugated biocytin to look at transport but I wanted to examine only retrograde transport and biocytin appears to be moving retrograde and anterograde. What should I do?
Do you have a neuronal tracer similar to Lucifer Yellow but in another fluorescent color?
How do I know which tracer to choose for my experiment?
Citations & References (11)
Citations & References
Abstract
Connexin26 is responsible for anionic molecule permeability in the cochlea for intercellular signalling and metabolic communications.
Journal:Eur J Neurosci
PubMed ID:15869481
'Abstract A gap junction is composed of two hemichannels and possesses a relatively large pore size ( approximately 10-15 A), allowing passage of ions and molecules up to 1 kDa. Here, we report that connexin hemichannels and gap junctions in the guinea pig cochlea had significant charge selectivity among permeating
Patch-clamp recordings from white matter glia in thin longitudinal slices of adult rat spinal cord.
Journal:J Neurosci Methods
PubMed ID:12100981
'We developed a technique of whole cell patch-clamp recordings from white matter oligodendrocytes and astrocytes in 200-250 microm-thick horizontal slices of adult (>2 months, 240-260 g) rat thoracic spinal cord. The viability of the white matter, sectioned in Na(+)-free, low Ca(2+) media, and the function of axons were preserved for
Gap junctional communication in the early Xenopus embryo.
Journal:J Cell Biol
PubMed ID:10953017
'In the Xenopus embryo, blastomeres are joined by gap junctions that allow the movement of small molecules between neighboring cells. Previous studies using Lucifer yellow (LY) have reported asymmetries in the patterns of junctional communication suggesting involvement in dorso-ventral patterning. To explore that relationship, we systematically compared the transfer of
Extracellular diffusivity determines contribution of high-versus low-affinity receptors to neural signaling.
Journal:Neuroimage
PubMed ID:15734347
'Diffusion-weighted magnetic resonance imaging detects physiological changes in the human brain by highlighting alterations in local diffusivity. However, the causal link between brain tissue diffusivity and neural activity is poorly understood. Synaptic physiology studies in vitro coupled with biophysical modeling have suggested that extracellular diffusion affects the spatial profile of
Properties of human connexin 31, which is implicated in hereditary dermatological disease and deafness.
Journal:Proc Natl Acad Sci U S A
PubMed ID:16549784
'The connexins are a family of at least 20 homologous proteins in humans that form aqueous channels connecting the interiors of coupled cells and mediating electrical and chemical communication. Mutations in the gene for human connexin 31 (hCx31) are associated with disorders of the skin and auditory system. Alterations in