Poly-HRP Streptavidin
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Thermo Scientific™

Poly-HRP Streptavidin

Poly-HRP Streptavidin consists of streptavidin protein that is covalently conjugated to a polymerized form of horseradish peroxidase (HRP) enzyme. StreptavidinRead more
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Catalog NumberQuantity
N2000.25 mL
Catalog number N200
Price (EUR)
350,00
Each
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Quantity:
0.25 mL
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Price (EUR)
350,00
Each
Add to cart
Poly-HRP Streptavidin consists of streptavidin protein that is covalently conjugated to a polymerized form of horseradish peroxidase (HRP) enzyme. Streptavidin binds to biotin and the conjugated HRP polymer provides a high level of activity for detection using an appropriate substrate system. This particular Poly-HRP Streptavidin product has been used primarily in sandwich ELISA applications to improve the signal provided by typical (non-Poly) streptavidin-HRP conjugates.

Features of Poly-HRP Streptavidin:

Formulation: Conjugate in PBS containing distilled glycerol, BSA and a preservative.
Application: ELISA: to be used with biotin-labeled detection antibodies. Recommended dilution range is 1:2,000 - 1:20,000. The optimal dilution must be determined empirically for each specific application. Prepare dilutions immediately before use.

Related Products
Poly-HRP Dilution Buffer
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration1.0 mg/mL
Label or DyeHRP (Horseradish Peroxidase)
Product TypeStreptavidin Conjugate
Quantity0.25 mL
Target MoleculeBiotin
ConjugateHRP
FormLiquid
Unit SizeEach
Contents & Storage
Do not store below -20°C. Avoid multiple freeze-thaw cycles.

Frequently asked questions (FAQs)

What is the concentration of Poly-HRP Streptavidin (Cat. No. N200) and what is the labelling ratio of HRP:Streptavidin?

The concentration of Poly-HRP Streptavidin is 1 mg/mL. The HRP:Streptavidin ratio is 5:1.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center

Can Poly-HRP Streptavidin (Cat. No. N200) be stored at -80 degrees C to increase its shelf life?

We do not recommend storing Poly-HRP Streptavidin at -80 degrees C, as the product can freeze solid at temperatures below -25 degrees C even if it has 50% glycerol as a stabilizer, thereby necessitating thawing of the product. With multiple freeze-thaws, the enzyme conjugates are rendered unstable.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center