FluoZin™-3, AM, cell permeant

Citations & References (43)

Invitrogen™

FluoZin™-3, AM, cell permeant

The FluoZin™-3 indicator is a Zn2+-selective indicator with a structure that resembles fluo-4. FluoZin™-3 exhibits high Zn2+-binding affinity that isRead more

Catalog Number	Quantity
F24195	100 μg

Catalog number F24195

Price (EUR)

764,00

Add to cart

Quantity:

100 μg

Price (EUR)

764,00

Add to cart

The FluoZin™-3 indicator is a Zn²⁺-selective indicator with a structure that resembles fluo-4. FluoZin™-3 exhibits high Zn²⁺-binding affinity that is unperturbed by Ca²⁺ concentrations up to at least 1 μM. In addition, FluoZin™-3 exhibits a >50-fold increase in fluorescence in response to saturating levels of Zn²⁺. Although imaging applications of FluoZin™-3 are predominant, cell-based microplate assays and flow cytometry protocols have also been developed. FluoZin™-3 indicator is available in a cell-permeant and a cell-impermeant form.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

Fluorescent Zinc Indicators Specifications:
• Label (Ex/Em): FluoZin™-3 (∼494/516 nm)
• K_d (Zn²⁺) (in buffer): ∼15 nM
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding the dissolved indicator to medium containing cells

Find Fluorescent Indicators for Zinc and Other Metal Ions
We offer a number of fluorescent indicators to determine polyvalent cation concentrations inside cells, to follow metal ion transport through ion channels, to make measurements in environmental samples, and more. Review Fluorescent Indicators for Zn²⁺ and Other Metal Ions—Section 19.7 in the Molecular Probes™ Handbook for more information on these products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeZinc Indicator

Molecular Weight (g/mol)982.85 g/mol

Quantity100 μg

Shipping ConditionRoom Temperature

For Use With (Application)Cell Viability and Proliferation

For Use With (Equipment)Fluorescence Microscope

Product LineFluoZin

Product TypeZinc Indicator

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (43)

Citations & References

Abstract

Demand for Zn2+ in acid-secreting gastric mucosa and its requirement for intracellular Ca2+.

Authors:Liu J, Kohler JE, Blass AL, Moncaster JA, Mocofanescu A, Marcus MA, Blakely EA, Bjornstad KA, Amarasiriwardena C, Casey N, Goldstein LE, Soybel DI,

Journal:PLoS One

PubMed ID:21698273

'Recent work has suggested that Zn(2+) plays a critical role in regulating acidity within the secretory compartments of isolated gastric glands. Here, we investigate the content, distribution and demand for Zn(2+) in gastric mucosa under baseline conditions and its regulation during secretory stimulation. Content and distribution of zinc were evaluated ... More

The amyloid precursor protein (APP) of Alzheimer disease and its paralog, APLP2, modulate the Cu/Zn-Nitric Oxide-catalyzed degradation of glypican-1 heparan sulfate in vivo.

Authors:Cappai R, Cheng F, Ciccotosto GD, Needham BE, Masters CL, Multhaup G, Fransson LA, Mani K

Journal:J Biol Chem

PubMed ID:15677459

'Processing of the recycling proteoglycan glypican-1 involves the release of its heparan sulfate chains by copper ion- and nitric oxide-catalyzed ascorbate-triggered autodegradation. The Alzheimer disease amyloid precursor protein (APP) and its paralogue, the amyloid precursor-like protein 2 (APLP2), contain copper ion-, zinc ion-, and heparan sulfate-binding domains. We have investigated ... More

Clioquinol and pyrithione activate TRPA1 by increasing intracellular Zn2+.

Authors:Andersson DA, Gentry C, Moss S, Bevan S,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19416844

'The antifungal and amoebicidal drug clioquinol (CQ) was withdrawn from the market when it was linked to an epidemic of subacute myelo-optico-neuropathy (SMON). Clioquinol exerts its anti-parasitic actions by acting as a Cu/Zn chelator and ionophore. Here we show that local injections of CQ produce mechanical hyperalgesia and cold hypersensitivity ... More

Intracellular zinc fluctuations modulate protein tyrosine phosphatase activity in insulin/insulin-like growth factor-1 signaling.

Authors:Haase H, Maret W

Journal:Exp Cell Res

PubMed ID:14644152

'Zinc is an effector of insulin/IGF-1 signaling and has insulinomimetic effects, the molecular basis of which is not understood. The present study establishes the capacity of zinc to inhibit protein tyrosine phosphatases (PTPs) as a cause for these effects and, moreover, demonstrates modulation of the insulin response by changes in ... More

Interleukin-6 regulates the zinc transporter Zip14 in liver and contributes to the hypozincemia of the acute-phase response.

Authors:Liuzzi JP, Lichten LA, Rivera S, Blanchard RK, Aydemir TB, Knutson MD, Ganz T, Cousins RJ

Journal:Proc Natl Acad Sci U S A

PubMed ID:15863613

'Infection and inflammation produce systemic responses that include hypozincemia and hypoferremia. The latter involves regulation of the iron transporter ferroportin 1 by hepcidin. The mechanism of reduced plasma zinc is not known. Transcripts of the two zinc transporter gene families (ZnT and Zip) were screened for regulation in mouse liver ... More

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