NeuroTrace™ DiI Tissue-Labeling Paste

Citations & References (11)

Invitrogen™

NeuroTrace™ DiI Tissue-Labeling Paste

NeuroTrace tissue labeling pastes consist of DiI, DiO (N-22881) or DiD (N-22882) mixed into an inert, water-resistant gel. The pastesRead more

Catalog Number	Quantity
N22880 also known as N-22880	500 mg

Catalog number N22880

also known as N-22880

Price (EUR)

422,00

Add to cart

Quantity:

500 mg

Price (EUR)

422,00

Add to cart

NeuroTrace tissue labeling pastes consist of DiI, DiO (N-22881) or DiD (N-22882) mixed into an inert, water-resistant gel. The pastes are ready to use as supplied and can be applied directly to live or fixed tissue specimens using the tip of a needle.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorYellow

Detection MethodFluorescence

For Use With (Equipment)Fluorescence Microscope

Product TypeTissue Labeling Paste

Quantity500 mg

Shipping ConditionRoom Temperature

Sub Cellular LocalizationCell Membranes & Lipids

Product LineNEUROTRACE

Unit SizeEach

Contents & Storage

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

I am doing retrograde neuronal labeling using the NeuroTrace DiI Paste. I apply a small glob onto the neuron of interest, but it is not adhering very well. What can I do?

This paste, which has DiI C₁₈ uniformly dispersed within a proprietary hydrophobic carrier, is commonly used to dab onto larger areas than smaller areas commonly used with crystals. Any moisture on the area the paste is applied may be the cause for lack of adherence. Apply the paste using a small flat-head spatula or toothpick and keep it in place a bit longer to ensure contact. The area may also be covered with parafilm to ensure contact as well.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I know which tracer to choose for my experiment?

Factors to consider are size of tracer, method of delivery (injection, direct application to tissue, etc.), and if the tracer needs to be fixable. Here are some links to details about the various classes of neuronal tracers we offer and how to choose between them:

Neuronal Tracing (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html)
Choosing a Tracer (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/choosing-a-tracer.html)
Imaging Analysis (http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/bioprobes-50-journal.pdf)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (11)

Citations & References

Abstract

In vivo mammalian brain imaging using one- and two-photon fluorescence microendoscopy.

Authors:Jung JC, Mehta AD, Aksay E, Stepnoski R, Schnitzer MJ

Journal:J Neurophysiol

PubMed ID:15128753

'One of the major limitations in the current set of techniques available to neuroscientists is a dearth of methods for imaging individual cells deep within the brains of live animals. To overcome this limitation, we developed two forms of minimally invasive fluorescence microendoscopy and tested their abilities to image cells ... More

Visualizing synapse formation in arborizing optic axons in vivo: dynamics and modulation by BDNF.

Authors:Alsina B, Vu T, Cohen-Cory S

Journal:Nat Neurosci

PubMed ID:11593233

'Dynamic developmental changes in axon arbor morphology may directly reflect the formation, stabilization and elimination of synapses. We used dual-color imaging to study, in the live, developing animal, the relationship between axon arborization and synapse formation at the single cell level, and to examine the participation of brain-derived neurotrophic factor ... More

Macrophages kill T9 glioma tumor cells bearing the membrane isoform of macrophage colony stimulating factor through a phagocytosis-dependent pathway.

Authors:Jadus MR, Williams CC, Avina MD, Ly M, Kim S, Liu Y, Narasaki R, Lowell CA, Wepsic HT

Journal:J Immunol

PubMed ID:9551992

Rat T9 glioma cells transfected with the gene for the membrane isoform of macrophage-CSF (mM-CSF) but not for the secreted isoform of M-CSF were directly killed by bone marrow-derived macrophages. Macrophage-mediated cytolysis of the mM-CSF-transfected clone was blocked by using chemical inhibitors of phagocytosis such as iodoacetate, 2-deoxyglucose, gadolinium chloride, ... More

Partition of membrane probes in a gel/fluid two-component lipid system: a fluorescence resonance energy transfer study.

Authors:Loura LM, Fedorov A, Prieto M

Journal:Biochim Biophys Acta

PubMed ID:10930513

A non-ideal lipid binary mixture (dilauroylphosphatidylcholine/distearoylphosphatidylcholine), which exhibits gel/fluid phase coexistence for wide temperature and composition ranges, was studied using photophysical techniques, namely fluorescence anisotropy, lifetime and resonance energy transfer (FRET) measurements. The FRET donor, N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-dilauroylphosphatidylethanol amine, and a short-tailed FRET acceptor, 1,1'-didodecil-3,3,3',3'-tetramethylindocarbocyanine (DiIC12(3)), were shown to prefer the fluid ... More

A discrete stage of baculovirus GP64-mediated membrane fusion.

Authors:Kingsley DH, Behbahani A, Rashtian A, Blissard GW, Zimmerberg J

Journal:Mol Biol Cell

PubMed ID:10588652

Viral fusion protein trimers can play a critical role in limiting lipids in membrane fusion. Because the trimeric oligomer of many viral fusion proteins is often stabilized by hydrophobic 4-3 heptad repeats, higher-order oligomers might be stabilized by similar sequences. There is a hydrophobic 4-3 heptad repeat contiguous to a ... More

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