NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm
Run Invitrogen Mini Protein Gels in Bio-Rad's Mini-PROTEAN® Tetra Cell. Click here for more information.
NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm
NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm
NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm
Citations & References (15)
Invitrogen™

NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity.
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Catalog NumberWellsGel ThicknessQuantity
NP0321BOX10-well1.0 mm10 gels (1 box)
NP0321PK210-well1.0 mm2 gels (1 box)
NP0322BOX12-well1.0 mm10 gels (1 box)
NP0322PK212-well1.0 mm2 gels (1 box)
NP0323BOX15-well1.0 mm10 gels (1 box)
NP0323PK215-well1.0 mm2 gels (1 box)
NP0324BOX1-well1.0 mm10 gels (1 box)
NP0326BOX2D-well1.0 mm10 gels (1 box)
NP0327BOX9-well1.0 mm10 gels (1 box)
NP0329BOX17-well1.0 mm10 gels (1 box)
NP0329PK217-well1.0 mm2 gels (1 box)
NP0330BOXIPG-well1.0 mm10 gels (1 box)
NP0335BOX10-well1.5 mm10 gels (1 box)
NP0335PK210-well1.5 mm2 gels (1 box)
NP0336BOX15-well1.5 mm10 gels (1 box)
NP0336PK215-well1.5 mm2 gels (1 box)
Catalog number NP0321BOX
Price (EUR)
179,65
Online Exclusive
222,00
Save 42,35 (19%)
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Wells:
10-well
Gel Thickness:
1.0 mm
Quantity:
10 gels (1 box)
Price (EUR)
179,65
Online Exclusive
222,00
Save 42,35 (19%)
Each
Add to cart
Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.

Features of NuPAGE Bis-Tris gels:
• Better protein integrity—optimized sample preparation process preserves your proteins
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Sample Reducing Agent (NP0004). Use NuPAGE Antioxidant (NP0005) in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer (NP00061) for traditional wet transfer using the Mini Blot Module (B1000) or XCell II Blot Module (EI9051). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Gel Thickness1.0 mm
Length (Metric)8 cm
Mode of SeparationMolecular Weight
Product LineNuPAGE
Quantity10 gels (1 box)
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 μL
Shelf Life12 Months
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Width (Metric)8 cm
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel TypeBis-Tris
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Wells10-well
Unit SizeEach

Frequently asked questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the advantage of NuPAGE Gels over regular Tris-Glycine gels?

The neutral operating pH of the NuPAGE Gels and buffers provides following advantages over the Laemmli system:
-Longer shelf life of 8-12 months due to improved gel stability
-Improved protein stability during electrophoresis at neutral pH resulting in sharper band resolution and accurate results (Moos et al, 1998)
-Complete reduction of disulfides under mild heating conditions (70 degrees C for 10 min) and absence of cleavage of asp-pro bonds using the NuPAGE LDS Sample buffer (pH > 7.0 at 70 degrees C)
-Reduced state of the proteins maintained during electrophoresis and blotting of the proteins by the NuPAGE Antioxidant
Please refer to the following paper: Moos M Jr, Nguyen NY, Liu TY (1988) Reproducible High Yield Sequencing of Proteins Electrophoretically Separated and Transferred to an Inert Support. J Biol Chem 263:6005-6008.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What is the maximum sample volume and concentration that may be loaded into the Invitrogen precast gel wells?

1-well, 1 mm: 700 µL (no more than 12 µg protein or 2.4 µg DNA per band)

2D-well, 1 mm: 400 µL or 1.5 mm: 600 µµL (no more than 12 µg protein or 2.0 µg DNA per band)

5-well, 1 mm: 60 µL (no more than 2 µg protein or 400 ng µg DNA per band)

9-well, 1 mm: 28 µL (no more than 0.5 µg protein or 100 ng DNA per band)

10-well, 1 mm: 25 µL or 1.5 mm: 37 µL (no more than 0.5 µg protein or 100 ng DNA per band)

12-well, 1 mm: 20 µL (no more than 0.5 µg protein or 100 ng DNA per band)

15-well, 1 mm: 15 µL or 1.5 mm: 25 µL (no more than 0.5 µg protein or 100 ng DNA per band)

17-well 1 mm: 15 µL (no more than 0.5 µg protein per band)

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm FAQs

Citations & References (15)

Citations & References
Abstract
The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores.
Authors:Fischer T, Strässer K, Rácz A, Rodriguez-Navarro S, Oppizzi M, Ihrig P, Lechner J, Hurt E,
Journal:EMBO J
PubMed ID:12411502
'Yra1p and Sub2p are components of the TREX complex, which couples transcription elongation with nuclear export of mRNAs. Here, we report a genetic interaction between Yra1p and a conserved protein Sac3p, which previously was found to interact with Sub2p. In vivo, Sac3p forms a stable complex with Thp1p, which was ... More
Rapid purification and mass spectrometric characterization of mitochondrial NADH dehydrogenase (Complex I) from rodent brain and a dopaminergic neuronal cell line.
Authors:Schilling B, Bharath M M S, Row RH, Murray J, Cusack MP, Capaldi RA, Freed CR, Prasad KN, Andersen JK, Gibson BW,
Journal:Mol Cell Proteomics
PubMed ID:15591592
'Oxidative stress and mitochondrial dysfunction signify important biochemical events associated with the loss of dopaminergic neurons in Parkinson''s disease (PD). Studies using in vitro and in vivo PD models or tissues from diseased patients have demonstrated a selective inhibition of mitochondrial NADH dehydrogenase (Complex I of the OXPHOS electron transport ... More
Intestinal trefoil factor confers colonic epithelial resistance to apoptosis
Authors:Taupin DR, Kinoshita K, Podolsky DK
Journal:Proc Natl Acad Sci U S A
PubMed ID:10639160
'Intestinal trefoil factor (ITF) is an essential regulator of colonic epithelial restitution, the rapid migration of colonocytes over mucosal wounds. High levels of ITF are frequently present in colorectal cancers and derived cell lines. Mucosal restitution requires the detachment of epithelium from substrate, which would be expected to induce apoptosis. ... More
2F3 monoclonal antibody recognizes the O26 O-antigen moiety of the lipopolysaccharide of enterohemorrhagic Escherichia coli strain 4276.
Authors:Szalo IM, Taminiau B, Goffaux F, Pirson V, McCappin J, Ball HJ, Mainil JG,
Journal:Clin Diagn Lab Immunol
PubMed ID:15138178
'Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) organisms are groups of pathogenic strains whose infections are characterized by a typical lesion of enterocyte attachment and effacement. They are involved in enteric diseases both in humans and in animals, and EHEC strains can be responsible for hemolytic uremic syndrome ... More
Human parainfluenza virus type 3 (PIV3) expressing the hemagglutinin protein of measles virus provides a potential method for immunization against measles virus and PIV3 in early infancy.
Authors:Durbin AP, Skiadopoulos MH, McAuliffe JM, Riggs JM, Surman SR, Collins PL, Murphy BR
Journal:J Virol
PubMed ID:10888621
'Recombinant human parainfluenza virus type 3 (PIV3) was used as a vector to express the major protective antigen of measles virus, the hemagglutinin (HA) glycoprotein, in order to create a bivalent PIV3-measles virus that can be administered intranasally. The measles virus HA open reading frame (ORF) was inserted as an ... More
View all NuPAGE™ Bis-Tris Mini Protein Gels, 4–12%, 1.0–1.5 mm citations