The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100 ng input DNA. In addition, highly efficient library construction enables the creation of 'amplification-free' libraries from 1 μg input material. This kit allows users to modulate an appropriate fragment insert size depending on desired read length and application.
The Ion Xpress™ Plus Fragment Library Kit contains sample preparation reagents for enzymatic shearing and library construction for up to 20 DNA libraries (depending on input DNA type) for semiconductor sequencing. Store components at -20°C.
The Ion Xpress™ Plus Fragment Library Kit offers you: • Superior coverage uniformity for diverse sample types using our proprietary Ion Shear™ Plus chemistry • Lower input requirement with as little as 100 ng starting material • Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes in as fast as 2 hours • Significant cost savings by removing the need for ancillary shearing devices • Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System
A Single-Day Workflow: The Next Stage in a Sequencing Revolution With a turnaround time of as little as 2 hours, the Ion Xpress™ Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your sample(s) of interest possible in less time than ever before. Furthermore, Ion Xpress™ Plus fragment libraries exhibit greater coverage uniformity and higher recovery rates across diverse sample types than other library construction methodologies, so you can be confident of generating the best data in the shortest time.
Ion Torrent Sequencing Made Simpler, Faster, and More Affordable Ion Shear™ Plus DNA fragmentation technology, included in the Ion Xpress™ Plus kit, obviates the need for costly physical shearing devices for most applications and requires as little as 100 ng of sample, accelerating your path to semiconductor sequencing. This flexible technology provides users the ability to adjust the resulting DNA fragment size to accommodate a range of sequencing read lengths depending on their project requirements. This technology is also amenable to automated library preparation systems such as the AB Library Builder™ System to provide quick answers when you need them most. Ion Shear™ Plus DNA fragmentation technology is not included in the Ion Plus kit. Use the Ion Plus kit if physical shearing methods are preferred.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Formato
Kit
Tipo de muestra
DNA
Sequencing Type
Genome and DNA Sequencing
Para utilizar con (equipo)
Ion PGM™ System, Ion OneTouch™ System
Para utilizar con (aplicación)
Sequencing
Compatibilidad de alto rendimiento
High-throughput Compatible, Multiplexing
Bibliotecas
Fragment Library
N.º de reacciones
10
Línea de productos
Ion Torrent™, Ion Xpress™
Tipo de producto
Plus Fragment Library Kit
Técnica
Post-Light™ Ion Semiconductor Sequencing
Compatibilidad de plantillas
Genomic DNA
Paso del flujo de trabajo
Library Generation
Contenido y almacenamiento
Ion Plus Fragment Library Kit & Ion Shear™ Plus Reagents Kit, –°C to –10°C
Figuras
Documentos y descargas
Certificados
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